A5103248718- Enzyme Catalysis and Immobilization
- Botanical Research and Applications
- Nanocomposite Films for Food Packaging
- Free Radicals and Antioxidants
- Polysaccharides and Plant Cell Walls
- Photochemistry and Electron Transfer Studies
- Microbial Metabolic Engineering and Bioproduction
- Phytochemicals and Antioxidant Activities
- Protein Structure and Dynamics
- Fermentation and Sensory Analysis
- Photochromic and Fluorescence Chemistry
China Agricultural University
2012-2025
Ministry of Agriculture and Rural Affairs
2024-2025
Beijing Academy of Food Sciences
2025
In this study, the phenomenon of stability-activity trade-off, which is increasingly recognized in enzyme engineering, was explored. Typically, enhanced stability enzymes correlates with diminished activity. Utilizing Rosa roxburghii copper–zinc superoxide dismutase (RrCuZnSOD) as a model, single-site mutations were introduced based on semirational design derived from consensus sequences. The initial set mutants selected activity, followed by combinatorial mutation. This approach yielded two...
Anthocyanins, anthocyanidins, 3-deoxyanthocyanins, and 3-deoxyanthocyanidins share five common chemical species in acidic media: flavylium cation, quinoidal base, hemiketal, cis trans chalcones. These are reversibly interconnected by four reactions: proton transfer, hydration, tautomerization, isomerization. The absence of a hydroxyl group or glycoside at position 3 the cation 3-deoxy substantially alters their thermodynamic kinetic properties compared to those anthocyanins. This study these...