A5004806592- Lipoproteins and Cardiovascular Health
- Protein Kinase Regulation and GTPase Signaling
- Genetic factors in colorectal cancer
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Glioma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- Nitric Oxide and Endothelin Effects
- Cancer, Hypoxia, and Metabolism
- Virus-based gene therapy research
- Cancer, Lipids, and Metabolism
- Genomics, phytochemicals, and oxidative stress
- Cardiac Ischemia and Reperfusion
- Hemophilia Treatment and Research
- melanin and skin pigmentation
- MicroRNA in disease regulation
- Cholesterol and Lipid Metabolism
- RNA Research and Splicing
- Coronary Artery Anomalies
- Metabolomics and Mass Spectrometry Studies
- Vascular Malformations Diagnosis and Treatment
- T-cell and Retrovirus Studies
- Genetic and Kidney Cyst Diseases
- Electrochemical sensors and biosensors
- Blood Coagulation and Thrombosis Mechanisms
Umm al-Qura University
2016-2025
Al Noor Hospitals
2022-2024
Ministry of Health of the Russian Federation
2024
King Saud bin Abdulaziz University for Health Sciences
2024
Alexandria University
2024
Neurological Surgery
2024
Maternity and Children's Hospital
2022
Virginia Commonwealth University Medical Center
1996-2007
Columbia University
2007
Virginia Commonwealth University
1998-2004
We have investigated oxidant-mediated stimulation of phospholipase D (PLD) activity in bovine pulmonary artery endothelial cells (BPAEC), prelabeled with [32P]orthophosphate or [32P]lysophospholipids. Treatment incubated Hanks' balanced salt solution (HBSS) containing 0.5% ethanol hydrogen peroxide (H2O2) linoleic acid hydroperoxide (18:2-OOH) enhanced the formation 32P-labeled phosphatidylethanol (PEt) and phosphatidic (PA) a dose- time-dependent manner, indicating activation PLD. The H2O2-...
Vital pulp therapy preserves and maintains the integrity health of dental tissue that has been injured by trauma, caries or restorative procedures. The enhancement cells viability formation reparative dentine new blood vessels are vital determinants success direct capping. Therefore, aims this study was to evaluate compare in vitro osteogenic, odontogenic angiogenic effects mineral trioxide aggregate (MTA), calcium hydroxide [Ca(OH)2], Biodentine Emdogain on stem (DPSCs) examine tested...
Acute systemic hypoxia induces delayed cardioprotection against ischemia (I)-reperfusion (R) injury via inducible nitric oxide synthase (iNOS)-dependent mechanism. Because CoCl2 is known to elicit hypoxia-like responses, we hypothesized that this chemical would mimic the preconditioning effect in heart. Adult male mice were pretreated with or saline. The hearts isolated 24 h later and subjected 20 min of global I 30 R Langendorff mode. Myocardial infarct size (% risk area; mean +/- SE,...
Adhesion and migration of tumor cells on through the vascular endothelium are critical steps metastatic invasion. We investigated roles E-selectin stress-activated protein kinase-2 (SAPK2/p38) in modulating endothelial adhesion transendothelial HT-29 colon carcinoma cells. Tumor necrosis factor α (TNFα) strongly increased expression human umbilical vein (HUVEC). This effect was independent activation SAPK2/p38 induced by TNFα. a monolayer HUVEC pretreated with TNFα dependent but activity...
We investigated the role of stress-activated p38 MAP kinase (p38/SAPK-2) signaling in delayed preconditioning heart. Adult male out-bred ICR mice were treated with activator, anisomycin (0.1 mg/kg IP), or vehicle (5% DMSO). Twenty-four hours later, hearts perfused Langendorff mode and subjected to 30 minutes ischemia reperfusion. Improvement postischemic recovery end-diastolic pressure reduction infarct size was observed, which abolished by SB203580, a specific inhibitor,...
Subtraction hybridization applied to a 'differentiation therapy' model of cancer employing human melanoma cells resulted in the cloning differentiation associated gene-7/interleukin-24 (mda-7/IL-24). Initial studies confirm an inverse correlation between mda-7 expression and development progression. Forced by means plasmid or via replication incompetent adenovirus (Ad.mda-7) promotes growth suppression induces apoptosis broad array cancers. In contrast, does not induce suppressive toxic...
Alzheimer’s disease is one of the main causes dementia among elderly individuals and leads to neurodegeneration different areas brain, resulting in memory impairments loss cognitive functions. Recently, a rare variant that associated with 3-fold higher risk onset has been found. The discovered missense mutation loop region exon 2 Trem2 (rs75932628-T, Arg47His). aim this study was investigate evidence for potential structural functional significance gene (Arg47His) through molecular dynamics...
Ultrasonography (US) is being recognized as a traditional way of the diagnosis various thyroid disorders, and this will help in detecting tumors early stage. Thyroid nodules are common usually benign; steps to diagnose malignancy should include careful clinical evaluation, laboratory tests, US exam fine-needle aspiration (FNA) biopsy.A total 173 registered cases were used for analysis study. Diagnosis was made following US-guided FNA cytology (FNAC) histopathological diagnosis;...
Genetic factors might influence metabolic syndrome (MetS) or any of its components. It was postulated that low density lipoprotein receptor (LDLR) gene variants could play a role in cholesterol hemostasis and the development MetS. However, causal-effect relationship between such MetS is not clearly identified even studied before Saudi Arabian women. This study aims to identify LDLR exon-4 women with comparison healthy assess expected effect amino acids alterations on structure functions...
Abstract Familial hypercholesterolemia (FH) is an autosomal dominant disease predominantly caused by a mutation in the low-density lipoprotein receptor ( LDLR ) gene. Here, we describe two severely affected FH patients who were resistant to statin therapy and managed on apheresis program. We identified novel duplication variant c.1332dup, p.(D445*) at exon 9 known silent c.1413A>G, p.(=), rs5930, NM_001195798.1 10 of gene both patients.
Purpose: Several recent studies have documented CTNNB1 and BRAF mutations which are mutually exclusive for adamantinomatous craniopharyngioma (ACP) papillary (PCP) tumors. This discovery is helpful in the development of novel targeted therapies successful clinical trials with PCP cases. However, no such therapy available yet ACP. Here, we report mutations, not previously reported, a case an adult ACP using NGS analysis. Results: Patient DNA was sequenced Ion PI v3 chip on Proton. A total 16...
<strong>Background:</strong> Familial hypercholesterolemia (FH) is an autosomal dominant inherited genetic disorder and results in the development of coronary artery disease (CAD). Clinical diagnosis homozygous HH patients usually straightforward because persistent can produce xanthoma corneal arcus. However, may also be misdiagnosed as skin lesions could therefore mistreated. The aim this case study report to highlight plight with FH means raising awareness condition among dermatologists...
Internal ribosome entry site (IRES) sequences have become a valuable tool in the construction of gene transfer and therapeutic vectors for multi-cistronic expression from single mRNA transcript. The optimal conditions effective use this sequence to construct functional vector are not precisely defined but it is generally assumed that internal dependent second such as cassette less efficient than cap-dependent first gene. Mainly tailoring inter-cistronic significantly enhances IRES...
Familial hypercholesterolemia (FH) is most commonly caused by mutations in the LDL receptor (LDLR), which responsible for hepatic clearance of from blood circulation. We described a severely affected FH proband and their first-degree relatives; was resistant to statin therapy managed on an apheresis program. In order find causative genetic variant this family, direct exon sequencing LDLR, APOB PCSK9 genes performed. identified compound heterozygous mutation with missense p.(W577C) frameshift...
Choroid plexus papilloma (CPP) is a rare benign tumor of the central nervous system that usually confined to cerebral ventricles. According World Health Organization, CPP corresponds grade I atypical (a-CPP); however, it can become more aggressive and reach II, which rarely undergo malignant transformation into choroid carcinoma (grade III). To best our knowledge, identification these tumors mutations by next generation DNA sequencing (NGS) has not been yet reported. In present study, NGS...
Lipoprotein Lipase (LPL) is known to be a key enzyme for lipid metabolism specifically in an enzymatic glycoprotein which provide tissues without fatty-acids and eliminates triglycerides (TG) by the circulation. Mutations LPL were proven cause alteration fractions within lipoprotein, causing development of atherosclerosis predispose weakening coronary artery disease (CAD) stroke. We examined linkage between genetic variant HindIII on lipoprotein fractions, stroke occurrences CAD. In this...