A5087824553- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Agricultural safety and regulations
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Healthcare Systems and Practices
- CRISPR and Genetic Engineering
- Health, Medicine and Society
- DNA and Nucleic Acid Chemistry
- CAR-T cell therapy research
- Genetically Modified Organisms Research
- Social Sciences and Governance
- Enzyme Structure and Function
- Viral Infectious Diseases and Gene Expression in Insects
- Cytomegalovirus and herpesvirus research
- Viral gastroenteritis research and epidemiology
- Cancer Research and Treatments
- Agriculture and Rural Development Research
- Biochemical and Molecular Research
- Immunotherapy and Immune Responses
- Escherichia coli research studies
- RNA Research and Splicing
- Intellectual Property and Patents
- Animal Genetics and Reproduction
Université Claude Bernard Lyon 1
2011-2023
École Normale Supérieure de Lyon
2010-2023
Centre International de Recherche en Infectiologie
1989-2023
Inserm
2009-2023
Centre National de la Recherche Scientifique
1999-2023
Hospital Arnau de Vilanova
2023
Hospital General Universitario de Alicante Doctor Balmis
2017
Virologie et Pathologies Humaines
2000-2014
Infectious Disease Models and Innovative Therapies
2011
École Normale Supérieure Paris-Saclay
2009
Using flow cytometry measurements combined with quantitative analysis of cell cycle kinetics, we show that rhesus monkey embryonic stem cells (ESCs) are characterized by an extremely rapid transit through the G1 phase, which accounts for 15% total duration. Monkey ESCs exhibit a non-phasic expression cyclin E, is detected during all phases cycle, and do not growth-arrest in after gamma-irradiation, reflecting absence checkpoint. Serum deprivation or pharmacological inhibition...
Lentivirus-derived vectors are very promising gene delivery systems since they able to transduce nonproliferating differentiated cells, while murine leukemia virus-based can only cycling cells. Here we report the construction and characterization of highly efficient minimal derived from simian immunodeficiency virus (SIVmac251). High-fidelity PCR amplification DNA fragments was used generate a SIV vector formed 5' cytomegalovirus early promoter, viral sequences up end gag required for...
As major antigen-presenting cells and effectors in the maintenance of tolerance, dendritic (DCs) are key immune system can thus be envisioned to have roles immunotherapy strategies. We, others, previously showed that simian immunodeficiency virus (SIV)-derived lentiviral vectors were able deliver a gene into human differentiated DCs. We describe here upgrading SIV vector improvements transduction protocol, which allowed us transduce more than 90% monocyte-derived developed new carrying...
BACKGROUNDAdiponectin is involved in the regulation of energy homeostasis and more recently reproductive functions. We have previously shown that adiponectin receptors (AdipoR1 AdipoR2) are expressed human granulosa cells. However, it remains to be investigated whether both AdipoR1 AdipoR2 or only one these serve as major receptor(s) for
Gene transfer into quiescent T and B cells is of importance for gene therapy immunotherapy approaches to correct hematopoietic disorders. Previously, we generated lentiviral vectors (LVs) pseudotyped with the Edmonston measles virus (MV) hemagglutinin fusion glycoproteins (Hgps Fgps) (H/F-LVs), which, first time, allowed efficient transduction human cells. These target express both MV entry receptors used by vaccinal strain, CD46 signaling lymphocyte activation molecule (SLAM)....
The fruR gene of Escherichia coli, which encodes the regulatory protein FruR, was cloned in pT7-5 expression vector so as to overproduce a tagged with 6 histidine residues. By using one-step chromatographic procedure, FruR purified near-homogeneity. Analysis under both denaturing and nondenaturing conditions indicated that it is tetramer molecular mass about 150 kilodaltons. positions interference between operator acetate operon were examined. number nature nucleotides essential for binding...
Primary Ciliary Dyskinesia is a heterogeneous genetic disease that characterized by cilia dysfunction of the epithelial cells lining respiratory tracts, resulting in recurrent tract infections. Despite lifelong physiological therapy and antibiotics, lungs affected patients are progressively destroyed, leading to insufficiency. Recessive mutations Dynein Axonemal Intermediate chain type 1 (DNAI1) gene have been described 10% cases Dyskinesia. Our goal was restore normal ciliary beating...
Nipah virus and Hendra are emerging, highly pathogenic, zoonotic paramyxoviruses that belong to the genus Henipavirus. They infect humans as well numerous mammalian species. Both viruses use ephrin-B2 -B3 cell entry receptors, following initial into an organism, they capable of rapid spread throughout host. We have previously reported can another attachment receptor, different from its bind nonpermissive circulating leukocytes, thereby promoting viral dissemination within Here, this molecule...
Abstract Background Lentiviral gene transfer into hematopoietic cells has been mostly optimized with vectors carrying a single reporter gene. For many clinical applications, lentiviral should contain more than one because transduced be enriched by selectable marker or killed for safety reasons after use. Thus, we compared various containing bicistronic cassette driven different ubiquitous promoters their ability to transduce human T‐lymphocytes, CD34 + ‐cells, and dendritic (DCs) derived...
IGF-I regulates pituitary and gonadal functions, is pivotal for sexual development fertility in mammalian species. To better understand the function of autocrine Sertoli cell physiology, we established a system Cre-mediated conditional inactivation receptor (IGF-IR) cultured cells. We show here that loss IGF-IR decreased number viable cells as consequence diminished proliferation increased death. Furthermore, lack altered morphology lactate transferrin secretions. Collectively, our data...
Abstract T cells represent a valuable tool for treating cancers and infectious inherited diseases; however, they are mainly short-lived in vivo. T-cell therapies would strongly benefit from gene transfer into long-lived persisting naive or progenitors. Here we demonstrate that baboon envelope glycoprotein pseudotyped lentiviral vectors (BaEV-LVs) far outperformed other LV pseudotypes transduction of adult fetal interleukin-7–stimulated cells. Remarkably, BaEV-LVs efficiently transduced...
FruR is an Escherichia coli transcriptional regulator that belongs to the LacI DNA-binding protein family. By using 1H and 15N NMR spectroscopy, we have determined three-dimensional solution structure of N-terminal domain consisting 57 amino acid residues. A total 809 NMR-derived distances 54 dihedral angle constraints been used for molecular modelling with X-PLOR program. The resulting set calculated structures presents average root-mean-square deviation 0.37 Å at main-chain level first 47...