A5044847522- SARS-CoV-2 and COVID-19 Research
- Hepatitis C virus research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- HIV Research and Treatment
- Hepatitis B Virus Studies
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Viral Infections and Outbreaks Research
- SARS-CoV-2 detection and testing
- RNA Interference and Gene Delivery
- Liver Disease Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
- Immunodeficiency and Autoimmune Disorders
- Systemic Lupus Erythematosus Research
- Endoplasmic Reticulum Stress and Disease
- interferon and immune responses
- Retinal and Optic Conditions
- Herpesvirus Infections and Treatments
- Lipid Membrane Structure and Behavior
- Influenza Virus Research Studies
- Mosquito-borne diseases and control
- Diabetes and associated disorders
Université Claude Bernard Lyon 1
2007-2024
Inserm
2006-2024
École Normale Supérieure de Lyon
2007-2024
Centre International de Recherche en Infectiologie
2016-2024
Centre National de la Recherche Scientifique
2016-2024
Université Jean Monnet
2021
Rensselaer Polytechnic Institute
2006
University of Freiburg
2006
Fujirebio (Belgium)
2006
Structure Fédérative de Recherche Biosciences
2006
Understanding the immune responses elicited by SARS-CoV-2 infection is critical in terms of protection against reinfection and, thus, for public health policy and vaccine development COVID-19. In this study, using either live particles or retroviruses pseudotyped with S viral surface protein (Spike), we studied neutralizing antibody (nAb) response serum samples from a cohort 140 qPCR-confirmed infections, including patients mild symptoms also more severe forms, those that required intensive...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a β-coronavirus, is the causative agent of COVID-19 pandemic. Like for other coronaviruses, its particles are composed four structural proteins: spike (S), envelope (E), membrane (M), and nucleoprotein (N) proteins. involvement each these proteins their interactions critical assembly production β-coronavirus particles. Here, we sought to characterize interplay SARS-CoV-2 during viral process. By combining biochemical imaging...
Following severe adverse reactions to the AstraZeneca ChAdOx1-S-nCoV-19 vaccine1,2, European health authorities recommended that patients under age of 55 years who received one dose receive a second Pfizer BNT162b2 vaccine as booster. However, effectiveness and immunogenicity this vaccination regimen have not been formally tested. Here we show heterologous combination confers better protection against acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection than homologous in...
In a survey of household cats and dogs laboratory-confirmed COVID-19 patients, we found high seroprevalence SARS-CoV-2 antibodies, ranging from 21% to 53%, depending on the positivity criteria chosen. Seropositivity was significantly greater among pets COVID-19+ households compared those with owners unknown status. Our results highlight potential role in spread epidemic.
Cellular binding and entry of hepatitis C virus (HCV) are the first steps viral infection represent a major target for antiviral antibodies novel therapeutic strategies. We have recently demonstrated that heparan sulfate (HS) plays key role in HCV envelope glycoprotein E2 to cells (Barth et al., J. Biol. Chem. 278:41003-41012, 2003). In this study, we characterized HCV-HS interaction analyzed its inhibition by host immune responses. Using recombinant glycoproteins, virus-like particles,...
Hepatitis C virus (HCV) assembly remains a poorly understood process. Lipid droplets (LDs) are thought to act as platforms for the of viral components. The JFH1 HCV strain replicates and assembles in association with LD-associated membranes, around which core protein is predominantly detected. In contrast, despite its intrinsic capacity localize LDs when expressed individually, we found that high-titer Jc1 recombinant was hardly detected on cell culture-grown (HCVcc)-infected cells, but...
Abstract Hepatitis D virus (HDV) doesn’t encode envelope proteins for packaging of its ribonucleoprotein (RNP) and typically relies on the surface glycoproteins (GPs) from hepatitis B (HBV) virion assembly, envelopment cellular transmission. HDV RNA genome can efficiently replicate in different tissues species, raising possibility that it evolved, and/or is still able to transmit, independently HBV. Here we show alternative, HBV-unrelated viruses act as helper HDV. In vitro, GPs several...
As major antigen-presenting cells and effectors in the maintenance of tolerance, dendritic (DCs) are key immune system can thus be envisioned to have roles immunotherapy strategies. We, others, previously showed that simian immunodeficiency virus (SIV)-derived lentiviral vectors were able deliver a gene into human differentiated DCs. We describe here upgrading SIV vector improvements transduction protocol, which allowed us transduce more than 90% monocyte-derived developed new carrying...
Cell entry of hepatitis C virus (HCV) is strikingly linked to lipoproteins and their receptors. Particularly, high density lipoprotein (HDL) enhances infectivity HCV by involving the lipid-transfer function scavenger receptor BI, a for both HDL HCV. Here, we demonstrate that apoC-I, an exchangeable apolipoprotein predominantly resides in HDL, specifically HCVcc HCVpp increases fusion rates between viral target membranes via direct interaction with surface. We identify hypervariable region 1,...
Syncytin is a fusogenic protein involved in the formation of placental syncytiotrophoblast layer. This encoded by envelope gene ERVWE1 proviral locus belonging to human endogenous retrovirus W (HERV-W) family. The HERV-W infectious ancestor entered primate lineage 25 40 million years ago. Although syncytin fusion property has been clearly demonstrated, little known about this cellular maturation process with respect classical proteins. Here we show that synthesized as glycosylated gPr73...
The spike protein (S) of severe acute respiratory syndrome coronavirus (SARS-CoV) is responsible for receptor binding and membrane fusion. It contains a highly conserved transmembrane domain that consists three parts: an N-terminal tryptophan-rich domain, central cysteine-rich C-terminal domain. cytoplasmic tail S has previously been shown to be required assembly. Here, the roles domains in infectivity fusion activity SARS-CoV have studied. S-pseudotyped retrovirus (SARSpp) was used measure...
Abstract The Crimean-Congo hemorrhagic fever virus (CCHFV) is an emerging pathogen of the Orthonairovirus genus that can cause severe and often lethal diseases in humans. CCHFV has a broad tropism infect variety species tissues. Here, by using gene silencing, blocking antibodies or soluble receptor fragments, we identify low-density lipoprotein (LDL-R) as entry factor. LDL-R facilitates binding particles but does not allow Hazara (HAZV), another member genus. In addition, show apolipoprotein...
ABSTRACT Cell entry of retroviruses is initiated by the recognition cellular receptors and subsequent membrane fusion between viral membranes. These two steps are mediated surface (SU) transmembrane (TM) subunits retroviral envelope glycoprotein (Env), respectively. Determinants regulating have been described throughout SU TM, but processes coupling receptor to still elusive. Here we establish that a critical interaction formed receptor-binding domain (RBD) major disulfide loop...
Summary There is an urgent need to develop novel approaches vaccination against the emerging, highly pathogenic avian influenza viruses. Here, we engineered viral-like particles (Flu-VLPs) derived from retroviral core that mimic properties of viral surface two viruses either H7N1 or H5N1 antigenic subtype. We demonstrate that, upon recovery RNAs a field strain, one can easily generate expression vectors encode HA, NA and M2 proteins virus prepare high-titre Flu-VLPs. characterise these...
The capacity of the surface glycoproteins enveloped viruses to mediate virus/cell binding and membrane fusion requires a proper thiol/disulfide balance. Chemical manipulation their redox state using reducing agents or free sulfhydryl reagents affects interaction. Conversely, natural rearrangements often occur during cell interaction trigger fusogenicity, hence virus entry. We examined relationship between 20 cysteine residues SARS-CoV (severe acute respiratory syndrome coronavirus) Spike...
Cell entry of enveloped viruses relies on the fusion between viral and plasma or endosomal membranes, through a mechanism that is triggered by cellular signal. Here we used combination computational experimental approaches to unravel main determinants hepatitis B virus (HBV) membrane process. We discovered ERp57 host factor critically involved in triggering HBV infection. Then, modeling approaches, uncovered putative allosteric cross-strand disulfide (CSD) bond S glycoprotein demonstrate its...
Abstract In a survey of household cats and dogs laboratory-confirmed COVID-19 patients, we found high seroprevalence SARS-CoV-2 antibodies, ranging from 21% to 53%, depending on the positivity criteria chosen. Seropositivity was significantly greater among pets COVID-19+ households compared those with owners unknown status. Our results highlight potential role in spread epidemic.
Despite the probable zoonotic origin of SARS-CoV-2, only limited research efforts have been made to understand role companion animals in SARS-CoV-2 epidemiology. According recent serological prevalence studies, human-to-companion animal transmission is quite frequent, which led us consider that risk from human, albeit negligible present context, may underestimated. In this study, we provide results a prospective survey was conducted evaluate isolation rate by qRT-PCR dogs and cats with...
Abstract Understanding the immune responses elicited by SARS-CoV-2 infection is critical in terms of protection from re-infection and, thus, for public health policy and vaccine development against COVID-19. Here, using either live particles or retroviruses pseudotyped with S viral surface protein (Spike), we studied neutralizing antibody (nAb) response serum specimens a cohort 140 qPCR-confirmed patients, including patient mild symptoms but also more severe form those that require intensive...
We report here a targeting method that exploits the expression pattern of cell surface proteases to induce gene delivery specific tissues. describe retroviral vectors harboring modified glycoproteins derived from an avian influenza virus hemagglutinin (HA) for which entry properties, dependent on HA cleavage by producer cells, were conditionally blocked at postbinding step insertion matrix metalloproteinase (MMP) substrates. demonstrate such transfer, both in vitro and mice human tumor...
Envelope glycoproteins (Env) of retroviruses are trimers SU (surface) and TM (transmembrane) heterodimers expressed on virions in fusion-competent forms that likely to be metastable. Activation the viral receptor-binding domain (RBD) via its interaction with a cell surface receptor is thought initiate cascade events lead refolding Env glycoprotein into stable fusion-active conformation. While conformation subunit has been described detail for several retroviruses, little known about...
Viroporins are small transmembrane proteins with ion channel activities modulating properties of intracellular membranes that have diverse proviral functions. Hepatitis C virus (HCV) encodes a viroporin, p7, acting during assembly, envelopment and secretion viral particles (VP). HCV p7 is released from the polyprotein through cleavage at E2-p7 p7-NS2 junctions by signal peptidase, but also exists as an E2p7 precursor, poorly defined properties. Here, we found ectopic expression in...