A5003056987- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Telomeres, Telomerase, and Senescence
- IL-33, ST2, and ILC Pathways
- CAR-T cell therapy research
- vaccines and immunoinformatics approaches
- Neuroinflammation and Neurodegeneration Mechanisms
- Virus-based gene therapy research
- Influenza Virus Research Studies
- Immune cells in cancer
- interferon and immune responses
- Inflammasome and immune disorders
- Kawasaki Disease and Coronary Complications
- Immune Response and Inflammation
- Immune responses and vaccinations
- Cytomegalovirus and herpesvirus research
- COVID-19 Impact on Reproduction
- Bioinformatics and Genomic Networks
- Bacteriophages and microbial interactions
- PARP inhibition in cancer therapy
- Retinoids in leukemia and cellular processes
Inserm
2013-2025
École Normale Supérieure de Lyon
2013-2025
Centre National de la Recherche Scientifique
2016-2025
Université Claude Bernard Lyon 1
2015-2025
Centre International de Recherche en Infectiologie
2013-2024
Université Jean Monnet
2021-2023
Immunité et Cancer
2017
Structure Fédérative de Recherche Biosciences
2012-2015
Université de Lyon
2009
Blocking TGF-β signaling in natural killer cells enhances their metabolism and ability to kill tumor cells.
The mouse inbred line C57BL/6J is widely used in genetics and its genome has been incorporated into many genetic reference populations. More recently large initiatives such as the International Knockout Mouse Consortium (IKMC) are using C57BL/6N strain to generate null alleles for all genes. Hence both strains now studies. Here we perform a comprehensive genomic phenotypic analysis of two identify differences that may influence their underlying mechanisms.We undertake sequence comparisons...
Following severe adverse reactions to the AstraZeneca ChAdOx1-S-nCoV-19 vaccine1,2, European health authorities recommended that patients under age of 55 years who received one dose receive a second Pfizer BNT162b2 vaccine as booster. However, effectiveness and immunogenicity this vaccination regimen have not been formally tested. Here we show heterologous combination confers better protection against acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection than homologous in...
Abstract Cellular senescence is induced by stresses and results in a stable proliferation arrest accompanied pro-inflammatory secretome. Senescent cells accumulate during aging, promoting various age-related pathologies limiting lifespan. The endoplasmic reticulum (ER) inositol 1,4,5-trisphosphate receptor, type 2 (ITPR2) calcium-release channel calcium fluxes from the ER to mitochondria are drivers of human cells. Here we show that Itpr2 knockout (KO) mice display improved aging such as...
MIS-C is characterized by a polyclonal activation of Vβ21.3 + CD4 and CD8 T cells that poorly respond to SARS-CoV-2 antigens.
The diversity of vaccination modalities and infection history are both variables that have an impact on the immune memory individuals vaccinated against SARS-CoV-2. To gain more accurate knowledge how these parameters imprint memory, we conducted a long-term follow-up SARS-CoV-2 spike protein–specific in unvaccinated COVID-19 convalescent as well infection-naïve individuals. Here, report from (hybrid immunity) group highest concentrations antibodies at 6 months after vaccination. As compared...
Abstract Autosomal recessive PRKCD deficiency has previously been associated with the development of systemic lupus erythematosus in human patients, but mechanisms underlying autoimmunity remain poorly understood. We introduced Prkcd G510S mutation that we to a Mendelian cause mouse genome, using CRISPR-Cas9 gene editing. PrkcdG510S/G510S mice recapitulated phenotype and had reduced lifespan. demonstrate this is linked B cell–autonomous role Prkcd. A detailed analysis cell activation shows...
Guanylate binding proteins (GBPs) are interferon-inducible involved in the cell-intrinsic immunity against numerous intracellular pathogens. The molecular mechanisms underlying potent antibacterial activity of GBPs still unclear. have been functionally linked to NLRP3, AIM2 and caspase-11 inflammasomes. Two opposing models currently proposed explain GBPs-inflammasome link: i) would target bacteria or bacteria-containing vacuoles increase cytosolic PAMPs release ii) directly facilitate...
Abstract Hutchinson–Gilford progeria syndrome (HGPS) is a lethal premature aging that recapitulates many normal characteristics. This disorder caused by mutation in the LMNA gene leading to production of progerin which induces misshapen nuclei, cellular senescence, and aging. We previously showed phospholipase A2 receptor (PLA2R1) promotes senescence induced replicative, oxidative, oncogenic stress but its role during progerin‐induced currently unknown. Here, we show knockdown PLA2R1...
Abstract Inactivated influenza vaccines (IIVs) lack broad efficacy. Cellular immunity to a conserved internal antigen, the nucleoprotein (NP), has been correlated protection against pandemic and seasonal thus could have potential broaden vaccine We developed OVX836, recombinant protein based on an oligomerized NP, which shows increased uptake by dendritic cells immunogenicity compared with NP. Intramuscular immunization in mice OVX836 induced strong NP-specific CD4+ CD8+ T-cell systemic...
Abstract Memory CD8 T cells typically exhibit improved effector functions compared to their naive counterparts. However, under certain activation conditions, such as chronic viral infections or cancer, these may develop functional defects. In this study, we the quality of memory generated following tumor rejection with those arising from an acute infection. We found that tumor-induced (Tum-CD8) exhibited a distinct phenotype and transcriptomic profile viral-induced (Vir-CD8) cells. These are...
SUMMARY Post-acute sequelae of COVID-19 (PASC) is a complex and multifaceted clinical challenge requiring to emphasize its underlying pathophysiological mechanisms. This study assessed hundreds virological, serological, immunological, tissue damage biomarkers in two patient cohorts who experienced mild (n=270) or severe (n=188) COVID-19, 6 9 months post-initial infection, which 40% 57.4% patients, respectively, developed PASC. Blood analysis showed that mains differences observed humoral,...
Abstract Objectives In the post‐SARS‐CoV‐2 pandemic era, “breakthrough infections” are still documented, due to variants of concerns (VoCs) emergence and waning humoral immunity. Despite widespread utilization, definition anti‐Spike (S) immunoglobulin‐G (IgG) threshold define protection has unveiled several limitations. Here, we explore advantages incorporating T‐cell response assessment enhance immune memory profile. Methods SARS‐CoV‐2 interferon‐gamma release assay test (IGRA) was...
IL-4 is one of the main cytokines produced during Th2-inducing pathologies. This cytokine has been shown to affect a number immune processes such as Th differentiation and innate responses. However, impact on CD8 T cell responses remains unclear. In this study, we analyzed effects global gene expression profiles Ag-induced memory cells in mouse. Gene ontology analysis signature revealed that regulated most importantly genes associated with Moreover, overlapped set preferentially expressed by...
Abstract The inflammasome is a signaling platform that central to the innate immune responses bacterial infections. Francisella tularensis bacterium replicating within host cytosol. During F. subspecies novicida infection, AIM2, an receptor sensing cytosolic DNA, activates caspase-1 in ASC-dependent manner, leading both pyroptosis and release of proinflammatory cytokines IL-1β IL-18. Activation this canonical pathway key limit infection. In study, by comparing AIM2 knockout (KO), ASCKO,...
Abstract The pool of memory-phenotype CD8 T cells is composed Ag-induced (AI) and cytokine-induced innate (IN) cells. IN have been described as having properties similar to those AI memory However, we found that pathogen-induced can be distinguished in mice from naturally generated by surface expression NKG2D. Using this marker, the increased functionalities compared with naive cells, shown comprehensive analysis cytokine secretion gene expression. differed their capacity migrate lung...
Tissue-resident memory (TRM) CD8+ T-cells play a crucial role in the protection against influenza infection but remain difficult to elicit using recombinant protein vaccines. OVX836 is vaccine, obtained by fusion of DNA sequence A nucleoprotein (NP) OVX313 heptamerization domain. We previously demonstrated that provides broad-spectrum viruses. Here, we show intramuscular (IM) immunization induces higher numbers NP-specific IFNγ-producing lung, compared mutant NP (NPm) and wild-type (NPwt),...
Most memory CD8 T cell subsets that have been hitherto defined are generated in response to infectious pathogens. In this study, we characterized the cells survive priming conditions, devoid of pathogen-derived danger signals. both a TCR-transgenic model and contact hypersensitivity, show naive under sterile inflammatory conditions generates memory. The corresponding can be identified by their intermediate expression levels CD44 CD122. We also CD44/122(int) spontaneously develop wild type...
Abstract Memory CD8 T lymphocyte populations are remarkably heterogeneous and differ in their ability to protect the host. In order identify whole range of qualities uniquely associated with protective memory cells we compared gene expression signatures two sharing same antigenic-specificity: (Influenza-induced, Flu-TM) non-protective (peptide-induced, TIM) spleen cells. Although Flu-TM TIM express classical phenotypic markers polyfunctional, only protects against a lethal viral challenge....
Although aging is a major risk factor for most types of cancers, it barely studied in this context. The transmembrane protein PLA2R1 (phospholipase A2 receptor) promotes cellular senescence, which can inhibit oncogene-induced tumor initiation. Functions and mechanisms action during are largely unknown. In study, we observed that old Pla2r1 knockout mice were more prone to spontaneously develop wide spectrum tumors compared control littermates. Consistently, these displayed increased Parp1,...
Prominent changes in the gut microbiota (referred to as "dysbiosis") play a key role development of allergic disorders, but underlying mechanisms remain unknown. Study delayed-type hypersensitivity (DTH) response mice contributed our knowledge pathophysiology human contact dermatitis. Here we report negative regulatory RIG-I-like receptor adaptor mitochondrial antiviral signaling (MAVS) on DTH by modulating bacterial ecology. Cohousing and fecal transplantation experiments revealed that dysbiotic