A5015143100- Ion Channels and Receptors
- Autophagy in Disease and Therapy
- Ion channel regulation and function
- Urinary Bladder and Prostate Research
- Telomeres, Telomerase, and Senescence
- Calcium signaling and nucleotide metabolism
- Neuroscience and Neural Engineering
- Renal cell carcinoma treatment
- Cell death mechanisms and regulation
- Cancer, Lipids, and Metabolism
- Bioactive Compounds and Antitumor Agents
- PARP inhibition in cancer therapy
- Erythrocyte Function and Pathophysiology
- Phytochemicals and Antioxidant Activities
- Asthma and respiratory diseases
- Magnesium in Health and Disease
- Endoplasmic Reticulum Stress and Disease
- Ferroptosis and cancer prognosis
- Neural Networks and Applications
- Cancer, Hypoxia, and Metabolism
- Pancreatic function and diabetes
- Cannabis and Cannabinoid Research
- Neuropeptides and Animal Physiology
- Circadian rhythm and melatonin
- Receptor Mechanisms and Signaling
Université de Lille
2015-2025
Inserm
2015-2025
Laboratoire de Physiologie Cellulaire
2017-2024
Laboratoire d'Excellence Canaux Ioniques d'Intérêt Thérapeutique
2023-2024
Sapienza University of Rome
2010-2015
Università di Camerino
2010-2014
University of Perugia
2012-2013
Abstract Cellular senescence is induced by stresses and results in a stable proliferation arrest accompanied pro-inflammatory secretome. Senescent cells accumulate during aging, promoting various age-related pathologies limiting lifespan. The endoplasmic reticulum (ER) inositol 1,4,5-trisphosphate receptor, type 2 (ITPR2) calcium-release channel calcium fluxes from the ER to mitochondria are drivers of human cells. Here we show that Itpr2 knockout (KO) mice display improved aging such as...
Abstract Cellular senescence is implicated in a great number of diseases including cancer. Although alterations mitochondrial metabolism were reported as drivers, the underlying mechanisms remain elusive. We report mechanism altering function and OXPHOS stress-induced senescent fibroblasts. demonstrate that TRPC3 protein, acting controller Ca 2+ load via negative regulation IP 3 receptor-mediated release, down regulated regardless type inducer. This remodelling promotes...
The aim of this study was to investigate the expression and function transient receptor potential vanilloid 2 (TRPV2) in human glioma cells. By Real-Time-PCR western blot analysis, we found that TRPV2 messenger RNA (mRNA) protein were expressed benign astrocyte tissues, its progressively declined high-grade tissues as histological grade increased (n = 49 cases), U87MG cells MZC, FCL FSL primary To glioma, small interfering used silence As evaluated by RT-Profiler PCR array, siTRPV2-U87MG...
The transient receptor potential vanilloid type-2 (TRPV2), belonging to the channel family, is a specialized ion expressed in human and other mammalian immune cells. This has been found be CD34(+) hematopoietic stem cells, where its cytosolic Ca(2) (+) activity crucial for stem/progenitor cell cycle progression, growth, differentiation. In innate TRPV2 granulocytes, macrophages, monocytes it stimulates fMet-Leu-Phe migration, zymosan-, immunoglobulin G-, complement-mediated phagocytosis,...
Abstract Malignant transformation of cells resulting from enhanced proliferation and aberrant differentiation is often accompanied by changes in transient receptor potential vanilloid (TRPV) channels expression. In gliomas, recent evidence indicates that TRPV type 2 ( TRPV2 ) negatively controls glioma cell survival proliferation. addition, cannabinoids, the ligands both cannabinoid receptors, promote glioblastoma stem‐like (GSCs) inhibit gliomagenesis. Herein, we provide on expression human...
Capsaicin (CPS) has been found to exhibit either tumor promoting or suppressing effects, many of which are mediated by the specific transient receptor potential vanilloid type-1 (TRPV1). Herein, we provide evidence that CPS treatment induced a more aggressive gene phenotype and invasiveness in 5637 cells-lacking TRPV1 receptor. cells upregulation pro-angiogenetic (angiopoietin 1, angiopoietin 2 vascular endothelial growth factor), pro-invasive pro-metastatic genes (MMP1, MMP9, TIMP1, TIMP3,...
Abstract TRPV1 activation and molecular mechanisms involved in the regulation of CPS-induced autophagy thymocytes. Autophagy is a highly conserved process lymphocyte development differentiation. Herein, we demonstrated for first time that triggering by specific agonist CPS induces mouse TRPV1-dependent required [Ca2+]i ROS generation, resulting AMPK activation. specifically increased Atg4C mRNA expression induced oxidation protein generation. TRPV1-triggered was Atg6/Beclin-1-dependent, as...
Tyrosine kinase inhibitors (TKI) such as sunitinib and pazopanib display their efficacy in a variety of solid tumours. However, use therapy is limited by the lack evidence about ability to induce cell death cancer cells. Our aim was evaluate cytotoxic effects induced 5637 J82 bladder lines.Cell viability tested MTT assay. Autophagy evaluated western blot using anti-LC3 anti-p62 antibodies, acridine orange staining FACS analysis. Oxygen radical generation necrosis were determined analysis...
Herein we evaluate the involvement of TRPV2 channel, belonging to Transient Receptor Potential Vanilloid channel family (TRPVs), in development and progression different tumor types. In normal cells, activation channels by growth factors, hormones, endocannabinoids induces a translocation receptor from endosomal compartment plasma membrane, which results abrogation cell proliferation induction death. Consequently, loss or inactivation signaling (e.g., glioblastomas), unchecked proliferation,...
T helper (T(H) )-17 lymphocytes are characterized by the expression of many regulatory cytokines, including IL-17A and IL-22, but at present no clinical data available on these cytokines in peripheral blood mononuclear cells (PBMC) from allergic asthmatic rhinitic (AR) children. The aim this study was to investigate a possible relationship between IL-22 mRNAs parameters seroatopic, AR, study, conducted during pollen season, included 18 healthy (n=9 n=9 rhinitic) Serum total specific IgE,...
There is evidence that calcium (Ca2+) increases the proliferation of human advanced prostate cancer (PCa) cells but ion channels involved are not fully understood. Here, we investigated correlation between alpha1D-adrenergic receptor (alpha1D-AR) and transient potential vanilloid type 1 (TRPV1) expression levels in PCa tissues evaluated ability alpha1D-AR to cross-talk with TRPV1 cell lines. The was examined by quantitative RT-PCR lines (DU145, PC3 LNCaP) cytofluorimetry. Moreover,...
Kalogris C, Caprodossi S, Amantini Lambertucci F, Nabissi M, Morelli M B, Farfariello V, Filosa A, Emiliozzi Mammana G & Santoni (2010) Histopathology 57 , 744–752 Expression of transient receptor potential vanilloid‐1 (TRPV1) in urothelial cancers human bladder: relation to clinicopathological and molecular parameters Aims: To evaluate the expression vanilloid type‐1 channel protein normal neoplastic tissues correlate TRPV1 with disease‐specific survival. Methods results: was analysed...
Abstract Pancreatic stellate cells (PSCs) that can co-metastasize with cancer shape the tumor microenvironment (TME) in pancreatic ductal adenocarcinoma (PDAC) by producing an excessive amount of extracellular matrix. This leads to a TME characterized increased tissue pressure, hypoxia, and acidity. Moreover, within secrete growth factors. The stimuli trigger Ca 2+ signaling cellular Na + loading. /Ca exchanger (NCX) connects homeostasis. NCX is electrogenic transporter, which shuffles 1...
Oxidative stress is an imbalance between the production of free radicals and antioxidant defense mechanisms, potentially leading to tissue damage. has a key role in development cerebrovascular and/or neurodegenerative diseases. This phenomenon mainly mediated by enhanced superoxide vascular endothelium with its consequent dysfunction. Thioctic, also known as alpha-lipoic acid (1,2-dithiolane-3-pentanoic acid), naturally occurring that neutralizes fatty watery regions cells. Both reduced...
The progression of normal cells to a tumorigenic and metastatic state involves the accumulation mutations in multiple key signaling proteins, encoded by oncogenes tumor suppressor genes. Recently, members TRP channel family have been included oncogenic protein family. TRPM1, TRPM8, TRPV6 are considered be suppressors localized melanoma prostate cancer, respectively. Herein, we focus our attention on antioncogenic properties TRPV1. Changes TRPV1 expression occur during development...
Autophagy and the ubiquitin-proteasome system (UPS) control thymus cell homeostasis under resting endoplasmic reticulum (ER) stress conditions. Several evidence support a cross-talk between UPS autophagy; abrogation of responses stimulates autophagy, vice versa inhibition autophagy alters functions. Herein, we found that TRPV1 activation induces ER stress, proteasome dysfunction in thymocytes by modulating expression UPR-related genes. The TRPV1-mediated prevents UPR inhibiting BiP, Grp94...
Sorafenib, a tyrosine kinase inhibitor, has been demonstrated to exert anti-tumor effects. However, the molecular mechanisms underlying its effects on bladder cancer remain unknown. Here, we evaluated responsible for sorafenib-induced 5637 and T24 cells. We that sorafenib reduces cell viability, stimulates lysosome permeabilization induces apoptosis of These are dependent by activation cathepsin B released from lysosomes. The sorafenib-increased activity induced proteolysis Bid into tBid...