A5066126463- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Alzheimer's disease research and treatments
- Endoplasmic Reticulum Stress and Disease
- Gut microbiota and health
- Tryptophan and brain disorders
- Metal complexes synthesis and properties
- Insect symbiosis and bacterial influences
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Ginseng Biological Effects and Applications
- Neurological diseases and metabolism
- Crystallography and molecular interactions
- Cholesterol and Lipid Metabolism
- Biochemical Analysis and Sensing Techniques
- Tannin, Tannase and Anticancer Activities
- Mosquito-borne diseases and control
- Peptidase Inhibition and Analysis
- Curcumin's Biomedical Applications
- Cellular transport and secretion
- Medicinal Plants and Neuroprotection
- Lipid metabolism and biosynthesis
- Retinoids in leukemia and cellular processes
- Regulation of Appetite and Obesity
- Histone Deacetylase Inhibitors Research
Università di Camerino
2016-2025
The University of Western Australia
2014
University of Kentucky
2006-2007
Gut microbiota has a proven role in regulating multiple neuro-chemical pathways through the highly interconnected gut-brain axis. Oral bacteriotherapy thus potential treatment of central nervous system-related pathologies, such as Alzheimer's disease (AD). Current AD treatments aim to prevent onset, delay progression and ameliorate symptoms. In this work, 3xTg-AD mice early stage were treated with SLAB51 probiotic formulation, thereby affecting composition gut its metabolites. This...
The gut-brain axis is a bidirectional communication network functionally linking the gut and central nervous system (CNS). Based on this, rational manipulation of intestinal microbiota represents novel attractive therapeutic strategy for treatment CNS-associated disorders. In this study, we explored properties probiotic formulation (namely SLAB51) in counteracting brain oxidative damages associated with Alzheimer's disease (AD). Specifically, transgenic AD mice (3xTg-AD) were treated SLAB51...
Cerebral glucose homeostasis deregulation has a role in the pathogenesis and progression of Alzheimer's disease (AD). Current therapies delay symptoms without definitively curing AD. We have previously shown that probiotics counteract AD 3xTg-AD mice modifying gut microbiota inducing energy metabolism glycolysis-gluconeogenesis. Ameliorated cognition is based on higher neuroprotective hormones concentrations, reduced amyloid-β burden, restored proteolytic pathways. Here, we demonstrate oral...
A series of ruthenium(II) arene complexes with the 4-(biphenyl-4-carbonyl)-3-methyl-1-phenyl-5-pyrazolonate ligand, and related 1,3,5-triaza-7-phosphaadamantane (PTA) derivatives, has been synthesized. The compounds have characterized by NMR IR spectroscopy, ESI mass spectrometry, elemental analysis, X-ray crystallography. Antiproliferative activity in four human cancer cell lines was determined MTT assay, yielding dose- line-dependent IC50 values 9-34 μM for three...
Gut microbiota has emerged as an important key regulator of health and disease status. Indeed, gut microbial dysbiosis been identified in increasing number diseases, including neurodegenerative disorders. Accordingly, alterations have reported also Alzheimer's (AD), suggesting possible pathogenetic mechanisms contributing to the development specific AD hallmarks exacerbating metabolic neuroinflammation. The identification these is crucial develop novel, targeted therapies identify potential...
In the present study we isolated proteasome complexes from control, mild cognitive impairment (MCI), and Alzheimer's disease (AD) subjects. No significant difference in amount of proteasomes was detected across different groups, although impairments chymotrypsin-like activity observed AD Large proteasome- mediated degradation an oxidized protein substrate MCI Incubation with a reducing agent (DTT) had no effect on activity, but fully restored proteasome-mediated Proteasomes subjects...
Abstract Organometallic ruthenium(II) complexes of general formula [(η 6 ‐arene)Ru(curcuminato)Cl], with arene being p ‐ i PrC H 4 Me ( 1 ), C 2 and 3 were synthesized, characterized, evaluated for their antitumor effects. Specifically, we explored ability to regulate the proteasome, a validated pharmacological target in cancer treatment. Ruthenium inhibited isolated proteasomes various extents, biological activity these depending on nature bound arene; particular, ‐arene)Ru(curcuminato)Cl]...
Cancer cells require both energy and material to survive duplicate in a competitive environment. Nutrients, such as amino acids (AAs), are not only caloric source, but can also modulate cell metabolism modify hormone homeostasis. Our hypothesis is that the environmental messages provided by AAs rule dynamics of cancer life or death, alteration balance between essential (EAAs) non-essential (NEAAs) (lower higher than 50%, respectively) present nutrients may represent key instrument alter...
Alzheimer's disease (AD) is a progressive neurodegeneration characterized by neuron death ending in memory and cognitive decline.A major concern AD research the identification of new therapeutics that could prevent or delay onset disorder, with current treatments being effective only reducing symptoms.In this perspective, use engineered probiotics as therapeutic tools for delivery molecules to eukaryotic cells finding application several disorders.This work introduces strategy treatment...
Alzheimer’s disease is a neurodegeneration with protein deposits, altered proteolysis, and inflammatory oxidative processes as major hallmarks. Despite the continuous search for potential therapeutic treatments, no cure available to date. The use of natural molecules adjuvants in treatment very promising strategy. In this regard, ginsenosides from ginseng root show variety biological effects. Here, we dissected role Rg1 Rg2 modulating autophagy stress neuroblastoma cells overexpressing...
Malaria control strategies are focusing on new approaches, such as the symbiotic control, which consists in use of microbial symbionts to prevent parasite development mosquito gut and block transmission infection humans. Several microbes, bacteria fungi, have been proposed for malaria or other mosquito-borne diseases strategies. Among these, yeast Wickerhamomyces anomalus has recently isolated from Anopheles mosquitoes, where it releases a natural antimicrobial toxin. Interestingly, many...
Alzheimer's disease (AD) is a progressive neurodegeneration characterized by extensive protein aggregation and deposition in the brain, associated with defective proteasomal autophagic-lysosomal proteolytic pathways. Since current drugs can only reduce specific symptoms, identification of novel treatments major concern AD research. Among natural compounds, (poly)phenols their derivatives/metabolites are emerging as candidates prevention due to multiple beneficial effects. This study aims...
Abstract Alzheimer’s disease (AD) is a progressive neurodegeneration with dysfunctions in both the ubiquitin–proteasome system (UPS) and autophagy. Astroglia participation AD an attractive topic of research, but molecular patterns are partially defined available vitro models have technical limitations. Immortalized astrocytes from hippocampus 3xTg-AD wild-type mice (3Tg-iAstro WT-iAstro, respectively) been obtained as attempt to overcome primary cell line limitations this study aims at...
Thymoquinone, a naturally derived agent, has been shown to possess antioxidant, antiproliferative and proapoptotic activities. In the present study, we explored thymoquinone effects on proteasomal complex, major system involved in removal of damaged, oxidized misfolded proteins. purified 20S complexes, subunit-dependent composition-dependent inhibition was observed, chymotrypsin-like trypsin-like activities were most susceptible treatment. U87 MG T98G malignant glioma cells treated with...