A5069135894- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Ovarian function and disorders
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Renal and related cancers
- Reproductive Health and Technologies
- CRISPR and Genetic Engineering
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive System and Pregnancy
- Reproductive biology and impacts on aquatic species
- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- Prenatal Screening and Diagnostics
- Neurogenesis and neuroplasticity mechanisms
- Tissue Engineering and Regenerative Medicine
- Epigenetics and DNA Methylation
- Primate Behavior and Ecology
- Effects and risks of endocrine disrupting chemicals
- Genetic Syndromes and Imprinting
- ATP Synthase and ATPases Research
- Hypothalamic control of reproductive hormones
- Proteoglycans and glycosaminoglycans research
- Proteins in Food Systems
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Oregon Health & Science University
2009-2020
Oregon National Primate Research Center
2008-2019
Institute of Livestock and Grassland Science
2004
University of Guelph
2004
Monash Institute of Medical Research
2004
Monash University
2004
Scientific and Technological Research Council of Turkey
2004
University College Dublin
2004
Royal Agricultural University
2004
Northwestern University
1998
Abstract Human ejaculated spermatozoa were washed through a Percoll gradient, preincubated for 10 hr in defined medium containing serum albumin, and then induced to undergo rapid acrosome reactions by addition of human follicular fluid or Sephadex G‐75 column fraction the fluid. Induction did not occur when only 0 5 hr. The detected indirect immunofluorescence using monoclonal antibody directed against sperm acrosomal region. percentage loss counted transmission electron microscopy agreed...
Genetically identical nonhuman primates can provide a powerful animal model for gene therapy and research activities where the physiological parameters directly or indirectly under study are heritable. Here we demonstrate that nuclear transfer is viable technology production of rhesus macaques. Oocytes recovered from gonadotropin-treated females were enucleated by aspiration first polar body underlying ooplasm, then activated cycloheximide exposure. Individual diploid blastomeres, in...
The role of albumin in mouse sperm capacitation was studied relation to its activities as a lipid-solubilizing protein and sterol acceptor. Two bovine serum albumins (BSA) which supported capacitation, Fraction V fatty acid-free, both contained cholesterol phospholipid but were without detectable levels high-density lipoprotein (HDL). lipid content BSA could be reduced by trichloroacetic acid (TCA) precipitation; however, removal all lipids required precipitation with ethanolic acetone...
Journal Article Endocrinology: Follicle stimulating hormone alone supports follicle growth and oocyte development in gonadotrophin-releasing antagonist-treated monkeys Get access M.B. Zelinski-Wooten, Zelinski-Wooten 1Oregon Regional Primate Research Center505 NW 185th Avenue, Beaverton, OR 97006 Search for other works by this author on: Oxford Academic PubMed Google Scholar J.S. Hutchison, Hutchison 2Ares Advanced Technology Inc. (Ares Serono), 280 Pond Street, Randolph, MA 02368 D.L. Hess,...
Using flow cytometry measurements combined with quantitative analysis of cell cycle kinetics, we show that rhesus monkey embryonic stem cells (ESCs) are characterized by an extremely rapid transit through the G1 phase, which accounts for 15% total duration. Monkey ESCs exhibit a non-phasic expression cyclin E, is detected during all phases cycle, and do not growth-arrest in after gamma-irradiation, reflecting absence checkpoint. Serum deprivation or pharmacological inhibition...
The occurence and time course of capacitation, acrosomal loss, hyperactivated motility require quantitative definition in order to characterize fertile human sperm. In this study, video microscopy digital image analysis were used measure curvilinear (VCL) straight line (VSL) velocity, average linearity progression (LIN [100 x VSL/VCL]), maximum mean amplitude lateral head displacement (ALH), beat-cross-frequency (BCF), DANCE (VCL meanALH) DANCEMEAN (meanALH/(LIN/100]. These parameters...
Twenty-three rhesus monkeys were subjected to 9 days of ovarian hyperstimulation with sequential exposure human follicle-stimulating hormone (hFSH) and then luteinizing (hLH) + hFSH. Six animals (26%) did not exhibit sustained, elevated levels circulating estradiol, primarily due the occurrence a premature surge endogenous LH (n = 4). Seventeen (74%) responded supraphysiologic estradiol (peak value: x̄ 4480 pg/ml) received chorionic gonadotropin (hCG) on Day 10. Oocytes collected 26 h later...
Progesterone receptors appear in granuloma cells of preovulatory follicles after the midcycle gonadotropin surge, suggesting important local actions progesterone during ovulation primates. Steroid reduction and replacement surge macaques was used to evaluate role ovulatory process. Animals received gonadotropins induce development multiple follicles, followed by human chorionic (hCG) administration (day 0) promote oocyte (nuclear) maturation, ovulation, follicular luteinization. On days 0-2,...
Previously we demonstrated high concentrations of desmosterol and docosahexaenoic acid (DHA, 22:6 n–<3) in monkey testes sperm. Desmosterol, a cholesterol precursor, is not present elsewhere the body. High DHA are found only retina brain. To examine distribution these compounds heads tails sperm, separated them determined their sterol, fatty acid, phospholipid molecular species composition. Desmosterol predominated (134.4 vs. 1.7 μg/109 cells heads). The content was also greater (66.2 30.3...
Sperm motility initiation, capacitation, and hyperactivation are modulated by an interplay of intracellular Ca2+, cAMP, pH. Mechanisms which these mediators alter sperm function have not been elucidated but may involve reversible alterations in regulatory protein phosphorylation. Studies were designed 1) to investigate the influence phosphatase (PP) inhibitor calyculin A (CA) on human 2) characterize CA-sensitive PP its endogenous regulators rhesus monkey sperm. CA (50 nM) treatment resulted...