A5037817244- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Metabolism and Genetic Disorders
- Reproductive Biology and Fertility
- Renal and related cancers
- Nutrition and Health in Aging
- Genetic Neurodegenerative Diseases
- ATP Synthase and ATPases Research
- Microtubule and mitosis dynamics
- Mesenchymal stem cell research
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Pancreatic function and diabetes
- Animal Genetics and Reproduction
- Congenital Diaphragmatic Hernia Studies
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Immune Cell Function and Interaction
- Healthcare Education and Workforce Issues
- CAR-T cell therapy research
- Evolution and Genetic Dynamics
- Genomics and Rare Diseases
- Genetic Syndromes and Imprinting
University of Ulsan
2019-2024
Asan Medical Center
2014-2024
Ulsan College
2019-2024
CHA University
2015-2024
CHA Bundang Medical Center
2015-2024
Oregon Health & Science University
2015-2019
Oregon National Primate Research Center
2015-2018
Institute for Basic Science
2014
Korea Food Research Institute
2010
Leaf senescence is a finely tuned and genetically programmed degeneration process, which critical to maximize plant fitness by remobilizing nutrients from senescing leaves newly developing organs. complex process that driven extensive reprogramming of global gene expression in highly coordinated manner. Understanding how regulatory networks involved controlling leaf are organized operated essential decipher the mechanisms senescence. It was previously reported trifurcate feed-forward pathway...
Range of DNA repair in response to double-strand breaks induced human preimplantation embryos remains uncertain due the complexity analyzing single- or few-cell samples. Sequencing such minute input requires a whole genome amplification that can introduce artifacts, including coverage nonuniformity, biases, and allelic dropouts at target site. We show here that, on average, 26.6% preexisting heterozygous loci control single blastomere samples appear as homozygous after indicative dropouts....
The bark of the root and stem Ulmus davidiana var. japonica has been used as a traditional Korean medicine to treat inflammatory disorders. This plant reportedly exhibits antioxidant, anticancer, anti-inflammatory effects. A search for biologically active compounds in U. extracts yielded bakuchiol, which we structurally identified on basis spectral data, including two-dimensional nuclear magnetic resonance spectroscopy distortionless enhancement by polarization transfer. In our study,...
Abstract Applications of genome editing ultimately depend on DNA repair triggered by targeted double-strand breaks (DSBs). However, mechanisms in human cells remain poorly understood and vary across different cell types. Here we report that DSBs selectively induced a mutant allele heterozygous embryos are repaired gene conversion using an intact wildtype homolog as template up to 40% embryos. We also show targeting homozygous loci facilitates interplay non-homologous end joining (NHEJ)...
What are the long-term developmental, reproductive and genetic consequences of mitochondrial replacement therapy (MRT) in primates?Longitudinal investigation MRT rhesus macaques (Macaca mulatta) generated with donor mtDNA that is exceedingly distant from original maternal counterpart suggest their growth, general health fertility unremarkable similar to controls.Mitochondrial gene mutations contribute a diverse range incurable human disorders. via spindle transfer oocytes was developed...
The accumulation of acquired mitochondrial genome (mtDNA) mutations with aging in somatic cells has been implicated dysfunction and linked to age-onset diseases humans. Here, we asked if mtDNA are also associated the mouse. MtDNA integrity multiple organs tissues young old (2–34 months) wild type (wt) mice was investigated by whole sequencing. Remarkably, no were detected tested tissues. However, identified several non-synonymous germline variants whose heteroplasmy levels (ratio normal...
Heritable mitochondrial DNA (mtDNA) mutations are common, yet only a few recurring pathogenic mtDNA variants account for the majority of known familial cases in humans. Purifying selection female germline is thought to be responsible elimination most harmful during oogenesis. Here we show that deleterious abundant ovulated mature mouse oocytes and preimplantation embryos recovered from PolG mutator females but not their live offspring. This implies purifying acts maternal per se,...
Poor survival of human pluripotent stem cells (hPSCs) following freezing, thawing, or passaging hinders the maintenance and differentiation cells. Rho-associated kinases (ROCKs) play a crucial role in hPSC survival. To date, typical ROCK inhibitor, Y-27632, has been primary agent used research. Here, we report that another fasudil, can be as an alternative is cheaper than Y-27632. It increased growth thawing passaging, like did not affect pluripotency, ability, chromosome integrity....
Abstract Haploidy is naturally observed in gametes; however, attempts of experimentally inducing haploidy somatic cells have not been successful. Here, we demonstrate that the replacement meiotic spindles mature metaphases II (MII) arrested oocytes with nuclei G0/G1 stage cell cycle results formation de novo consisting homologous chromosomes comprising single chromatids. Fertilization such sperm triggers extrusion one set into pseudo-polar body (PPB), resulting a zygote haploid and pronuclei...
Cells transmit their genomes vertically to daughter cells during cell divisions. Here, we demonstrate the occurrence and extent of horizontal mitochondrial (mt)DNA acquisition between that are not in a parent-offspring relationship. Extensive single-cell sequencing from various tissues organs adult chimeric mice composed carrying distinct mtDNA haplotypes showed substantial fraction individual cardiomyocytes, neurons, glia, intestinal, spleen captured donor at high levels. In addition,...
Patient-derived induced pluripotent stem cells (iPSCs) are materials that can be used for autologous cell therapy. We screened mtDNA mutations in iPSCs and iPSC-derived neuronal from patients with Alzheimer's disease (AD). Also, we investigated whether the could affect mitochondrial function deposition of β-amyloid (Aβ) differentiated cells.mtDNA were measured compared among cells. The selected carrying subcloned, then their growth rate differentiation pattern analyzed. respiration membrane...
Other SectionsABSTRACTINTRODUCTIONRESULTS AND DISCUSSIONCONCLUSIONSMATERIALS METHODSSupplementary InformationACKNOWLEDGEMENTSCONFLICTS OF INTERESTFIGURESREFERENCES
Defects in the mitochondrial genome (mitochondrial DNA (mtDNA)) are associated with both congenital and acquired disorders humans. Nuclear-encoded polymerase subunit gamma (POLG) plays an important role mtDNA replication, proofreading mutations POLG have been linked increased deletions. SSBP1 is also a crucial gene for replication. Here, we describe patient diagnosed Pearson syndrome large deletions that were not detected somatic cells of mother. Exome sequencing was used to evaluate nuclear...
Reproductive biotechnology has developed rapidly and is now able to overcome many birth difficulties due infertility or the transmission of genetic diseases. Here we introduce next generation assisted reproductive technologies (ART), such as mitochondrial replacement technique (MRT) correction in eggs with micromanipulation. Further, suggest that information from somatic cells subsequent generations without gametes should be useful for people who suffer Pluripotent stem (PSCs) can converted...
Abstract Background Amnion-derived mesenchymal stem cells (AM-MSCs) are an attractive source of cell therapy for patients with irreversible liver disease. However, there obstacles to their use due low efficiency and xeno-contamination hepatic differentiation. Methods We established efficient protocol differentiating AM-MSCs into progenitor (HPCs) by analyzing transcriptome-sequencing data. Furthermore, generate the xeno-free conditioned differentiation protocol, we replaced fetal bovine...
Yeon-Mi Lee, Seon Hyeong Kim, Young Shin Eun Mee Jung Yeon Min Ae Keum, Jae Moon, Su Jin Park, Dong Woo Shin, Hong-Yup Ahn, Ran Hae Jun Yim, Suk-Kyung Hong, Hyun Wook Baik. J Clin Nutr 2014;6:71-8. https://doi.org/10.15747/jcn.2014.6.2.71