A5013799594- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Genetic Neurodegenerative Diseases
- Neurogenesis and neuroplasticity mechanisms
- Mesenchymal stem cell research
- Mathematical Biology Tumor Growth
- Neuroscience and Neural Engineering
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Biomedical Ethics and Regulation
- Mitochondrial Function and Pathology
- Nerve injury and regeneration
- Developmental Biology and Gene Regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Muscle Physiology and Disorders
- Diverse Approaches in Healthcare and Education Studies
- Education and Learning Interventions
- S100 Proteins and Annexins
- Education, Safety, and Science Studies
- Renal and related cancers
- Cytokine Signaling Pathways and Interactions
- Educational Systems and Policies
- Hedgehog Signaling Pathway Studies
- Fibroblast Growth Factor Research
Hanyang University
2025
CHA University
2014-2023
Bio-Medical Science (South Korea)
2023
Jeonju National University of Education
2021
Sogang University
2010-2016
Stem Cell Institute
2014
Massachusetts General Hospital
1999-2002
Harvard University
1999-2002
Japanese Foundation For Cancer Research
2002
University of California, Irvine
2002
Use of clinical-grade human induced pluripotent stem cell (iPSC) lines as a starting material for the generation cellular therapeutics requires demonstration comparability derived from different individuals and in facilities. This agreement on critical quality attributes such assays that should be used. Working established recommendations guidance International Stem Cell Banking Initiative embryonic banking, concentrating those issues more relevant to iPSCs, series consensus workshops has...
Huntington’s disease (HD) is an autosomal dominant neurodegenerative disorder of the central nervous system (CNS) that defined by a CAG expansion in exon 1 huntingtin gene leading to production mutant (mHtt). To date, pathophysiology has been thought be primarily driven cell-autonomous mechanisms, but, here, we demonstrate fibroblasts derived from HD patients carrying either 72, 143 and 180 repeats as well induced pluripotent stem cells (iPSCs) also characterized can transmit protein...
ABSTRACT ActRIA (or ALK2), one of the type I receptors transforming growth factor-β (TGF-β) superfamily, can bind both activin and bone morphogenetic proteins (BMPs) in conjunction with BMP II receptors, respectively. In mice, is expressed primarily extraembryonic visceral endoderm before gastrulation later embryonic cells during gastrulation. To elucidate its function mouse development, we disrupted transmembrane domain by gene targeting. We showed that embryos homozygous for mutation were...
Induced pluripotent stem cells (iPSCs) generated from somatic of patients can be used to model different human diseases. They may also serve as sources transplantable that in novel cell therapies. Here, we analyzed neuronal properties an iPSC line derived a patient with juvenile form Huntington's disease (HD) carrying 72 CAG repeats (HD-iPSC). Although its initial neural inducing activity was lower than embryonic cells, found HD-iPSC give rise GABAergic striatal neurons, the type is most...
HD (Huntington's disease) is a devastating neurodegenerative genetic disorder caused by abnormal expansion of CAG repeats in the HTT (huntingtin) gene. We have recently established two iPSC (induced pluripotent stem cell) lines derived from patient carrying 72 (HD-iPSC). In order to understand proteomic profiles HD-iPSCs, we performed comparative analysis among normal hESCs (human embryonic cells; H9), iPSCs (551-8) and HD-iPSCs at undifferentiated stages, identified 26 up- down-regulated...
Malignant gliomas are the most common primary brain tumor in adults. A number of genes have been implicated glioblastoma including mutation and deletion PTEN. PTEN is a regulator PI3K-mediated Akt signaling pathways has recognized as therapeutic target glioblastoma. To achieve potent inhibition PI3K-Akt pathway glioblastoma, it essential to understand interplay between regulators its activation. Here, ectopic expression U-87MG human glioblastoma-astrocytoma cell line shown result depletion...
Abstract Although autologous induced pluripotent stem cells (iPSCs) can potentially be useful for treating patients without immune rejection, in reality it will extremely expensive and labor-intensive to make iPSCs realize personalized medicine. An alternative approach is use of human leukocyte antigen (HLA) haplotype homozygous donors provide HLA matched iPSC products significant numbers patients. To establish a haplobank iPSCs, we repurposed the cord blood bank by screening ∼4,200 high...
Ischemic stroke mainly caused by middle cerebral artery occlusion (MCAo) is a major type of stroke, but there are currently very limited therapeutic options for its cure. Neural stem cells (NSCs) or neural precursor (NPCs) derived from various sources known to survive and improve neurological functions when they engrafted in animal models stroke. Induced pluripotent (iPSCs) generated somatic patients novel that promise the autologous cell therapy In this study, we successfully differentiated...
Anteroposterior (AP) patterning of the developing CNS is crucial for both regional specification and timing neurogenesis. Several important factors are involved in AP patterning, including members WNT FGF growth factor families, retinoic acid receptors, HOX genes. We have examined interactions between signaling pathways. Blockade downregulates expression RAR pathway, RARalpha, RALDH2 CYP26. Overexpression a constitutively active RARalpha2 rescues effects blockade on XCAD3 HOXB9. This...
To understand the fates of human mesenchymal stem cells (hMSCs) following transplantation into a rodent model middle cerebral artery occlusion (MCAo), magnetic resonance imaging (MRI) techniques were employed, hMSCs labeled with ferumoxides (Feridex)--protamine sulfate complexes, which visualized and examined by MRI up to 10 weeks transplantation. Migration transplanted infarcted area was further confirmed histological methods. We found that in MCAo models possess capacity migrate...
Fluidic resonators with integrated microchannels (hollow resonators) are attractive for mass, density, and volume measurements of single micro/nanoparticles cells, yet their widespread use is limited by the complexity fabrication. Here we report a simple cost-effective approach fabricating hollow microtube resonators. A prestructured silicon wafer annealed at high temperature under controlled atmosphere to form self-assembled buried cavities. The interiors these cavities oxidized produce...
Endan Li1,#, Jiwoo Choi, Hye-Ri Sim, Jiyeon Kim, Jae Hyun Jun, Jangbeen Kyung, Nina Ha, Semi Kim,<br />Keun Ho Ryu, Seung Soo Chung, Sook Sungsu Lee, Wongi Seol & Jihwan Song. BMB Reports 2023;56:178-83. https://doi.org/10.5483/BMBRep.2022-0157
Ischemic stroke mainly caused by middle cerebral artery occlusion (MCAo) represents the major type of stroke; however, there are still very limited therapeutic options for stroke-damaged patients. In this study, we evaluated neurogenic and potentials human neural stem cells (NSCs) overexpressing brain-derived neurotrophic factor (HB1.F3.BDNF) following transplantation into a rodent model MCAo. F3.BDNF NSCs (F3.BDNF) were transplanted contralateral side striatum at 7 days after MCAo, animals...
Induced pluripotent stem cells (iPSCs) can be generated by introducing several factors into mature somatic cells. Banking of iPSCs lead to wider application for treatment and research. In an economical view, it is important store that cover a high percentage the population. Therefore, use homozygous human leukocyte antigen-iPSCs (HLA-iPSCs) thought as potential candidate effective iPSC banking system further clinical use. We screened database stored in Catholic Hematopoietic Stem Cell Bank...
Abstract Introduction There is no single blood marker for predicting the prognosis in ischemic stroke. A combination of multiple markers may enhance ability to predict long-term outcome following Methods Blood concentrations neuronal (neuron-specific enolase, visinin-like protein 1, heart type fatty acid binding (hFABP) and neuroglobin), astroglial (S100B glial fibrillary acidic protein), inflammatory (IL-6, TNF-α, C-reactive blood-brain barrier (matrix metalloproteinase 9), haemostatic...