A5021195541- Chronic Lymphocytic Leukemia Research
- Medical Imaging Techniques and Applications
- Immune Cell Function and Interaction
- Chronic Myeloid Leukemia Treatments
- CAR-T cell therapy research
- Hematopoietic Stem Cell Transplantation
- Advanced X-ray and CT Imaging
- T-cell and B-cell Immunology
- Digital Radiography and Breast Imaging
- Radiation Dose and Imaging
- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Acute Lymphoblastic Leukemia research
- Lanthanide and Transition Metal Complexes
- PI3K/AKT/mTOR signaling in cancer
- Cancer Genomics and Diagnostics
- Acute Myeloid Leukemia Research
- Vector-Borne Animal Diseases
- Immunodeficiency and Autoimmune Disorders
- Click Chemistry and Applications
- Cancer Mechanisms and Therapy
- Immunotherapy and Immune Responses
- Nanoplatforms for cancer theranostics
- Ophthalmology and Eye Disorders
- Mesenchymal stem cell research
University of Zurich
2024-2025
University of Helsinki
2017-2024
Helsinki University Hospital
2017-2024
Eulji University
2018
Seoul National University
2014
National Cancer Center
2014
Yonsei University
2011-2014
Korea Basic Science Institute
2007
University of Pennsylvania
2007
Abstract Interleukin-23 (IL-23) is a proinflammatory cytokine mainly produced by myeloid cells that promotes tumor growth in various preclinical cancer models and correlates with adverse outcomes. However, as to how IL-23 fuels unclear. Here, we found tumor-associated macrophages be the main source of mouse human microenvironments. Among IL-23-sensing cells, identified subset tumor-infiltrating regulatory T (T reg ) display highly suppressive phenotype across tumors. The use three solid...
Abstract The oncogenic protein Bcr-Abl has two major isoforms, p190 and p210 . While is the hallmark of chronic myeloid leukemia (CML), occurs in majority Philadelphia-positive acute lymphoblastic (Ph + ALL) patients. In CML, a minority patients associating with distinct hematological features inferior outcomes, yet pathogenic role potential targeting therapies are largely uncharacterized. We employed next generation sequencing, phospho-proteomic profiling, drug sensitivity testing to...
BCR::ABL1-independent pathways contribute to primary resistance tyrosine kinase inhibitor (TKI) treatment in chronic myeloid leukemia (CML) and play a role leukemic stem cell persistence. Here, we perform ex vivo drug screening of CML CD34
To understand the fates of human mesenchymal stem cells (hMSCs) following transplantation into a rodent model middle cerebral artery occlusion (MCAo), magnetic resonance imaging (MRI) techniques were employed, hMSCs labeled with ferumoxides (Feridex)--protamine sulfate complexes, which visualized and examined by MRI up to 10 weeks transplantation. Migration transplanted infarcted area was further confirmed histological methods. We found that in MCAo models possess capacity migrate...
Abstract CD4+ T-cell large granular lymphocyte leukemia (T-LGLL) is a rare subtype of T-LGLL with unknown etiology. In this study, we molecularly characterized cohort patients ( n = 35) by studying their receptor (TCR) repertoire and the presence somatic STAT5B mutations. addition to previously described gain-of-function mutations (N642H, Y665F, Q706L, S715F), discovered six novel (Q220H, E433K, T628S, P658R, P702A, V712E). Multiple were present in 22% (5/23) mutated cases, either coexisting...
Abstract CD4 + T cells play a critical role in antiviral humoral and cellular immune responses. We have previously reported that subcutaneous lymphocytic choriomeningitis virus (s.c. LCMV) infection is characterized by stark compartmentalization of cells, leading to strong H 1 cell polarization but virtually absent follicular helper (T FH ) key drivers immunity. Here, we investigate the mechanisms responsible for this impaired differentiation. show T-bet induced LCMV encompass subset...
Abstract Large granular lymphocyte leukemia (LGLL) is characterized by somatic gain-of-function STAT3 mutations. However, the functional effects of mutations on primary LGLL cells have not been studied in detail. In this study, we show that CD8+ T isolated from mutated patients high protein levels epigenetic regulators, such as DNMT1, and are global hypermethylation. Correspondingly, treatment healthy with IL-6, IL-15, and/or MCP-1 cytokines resulted activation, increased EZH2, c-MYC, l-MYC,...
Abstract Graft versus host disease (GvHD) is the main complication of allogeneic hematopoietic stem cell transplantation (HSCT). Here we report studies a patient with chronic GvHD (cGvHD) carrying persistent CD4 + T clonal expansion harboring somatic mTOR , NFKB2 and TLR2 mutations. In screening cohort (n = 134), detect P2229R kinase domain mutation in two additional cGvHD patients, but not healthy or HSCT patients without cGvHD. Functional analyses indicate gain-of-function alteration...
To evaluate the use of 5-aminolevulinic acid (5-ALA) for noninvasive detection malignant gliomas by using in vivo magnetic resonance (MR) imaging a mouse brain tumor model.The experiments were animal care committee approved. U-87 glioblastoma cells exposed to 5-ALA (500 µmol/L) 6 hours, harvested, and intracellular concentrations iron, heme, protoporphyrin IX, ferrochelatase measured (six each group). BALB/c nude mice (n = 10) inoculated with glioma produce orthotopic tumors. T2-weighted was...
Somatic mutations in T cells can cause cancer but also have implications for immunological diseases and cell therapies. The mutation spectrum nonmalignant is unclear. Here, we examined somatic CD4 + CD8 from 90 patients with hematological disorders used receptor (TCR) single-cell sequencing to link expansions phenotypes. had a higher burden than cells. Notably, the biggest variant allele frequency (VAF) of non-synonymous variants was synonymous cells, indicating non-random occurrence. VAF...
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Our aim in this study was to examine the resolution effects of breast thickness magnification technique by evaluating generalized modulation transfer function (GMTF) including effect focal spot, effective pixel size and scatter. The PMMAs ranging from 10 40 mm were placed on a standard supporting platform that positioned achieve factors 1.2 2.0. As increased, spot MTF degraded while detector improved. A small resulted an improvement GMTF due smaller magnification. In contrast, large...
Background In this study, we investigate the image quality of virtual monochromatic images synthesized from dual-energy computed tomography (DECT) at voltages 80/140 kV and 100/140 kV. Materials Methods Virtual a phantom are DECT scans 40 to 70 keV in steps 1 under two combinations tube voltages. The dose allocation (DE) scan is 50% for both low- high-energy tubes. compared single-energy (SE) same radiation dose. DE images, noise reduced using optimal energy. reconstructed 1-keV increments...
ABSTRACT Graft-versus-host-disease (GvHD) is the main complication of allogeneic hematopoietic stem cell transplantation. GvHD patients have aberrant T expansions, which are thought to drive pathological immune activation. Here we report mechanistic insights that somatic mutations may account for persistent clonal expansions in chronic (cGvHD). In an index patient suffering from cGVHD, discovered persisting MTOR , NFKB2 and TLR2 expanded CD4+ clone. screening cohort (n=135), P2229R kinase...
X-ray mammography can be used to acquire images useful for early breast cancer detection in medical applications. There have been a number of approaches diagnostic low-dose mammography. The purpose this study was design concept use monochromatic beams by using K-edge filters low dose SRS-78 simulation code and Monte Carlo GATE were model the tube, target material, filter phantom, detector. To achieve beam, molybdenum (Mo) rhodium (Rh) as materials various thicknesses. direct conversion...