A5102927814- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Long-Term Effects of COVID-19
- Plant tissue culture and regeneration
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Chemokine receptors and signaling
- Immune cells in cancer
- Transgenic Plants and Applications
- Cell death mechanisms and regulation
- CAR-T cell therapy research
- Hepatitis B Virus Studies
- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Reproductive Biology and Fertility
- interferon and immune responses
- Liver Disease Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
- Prenatal Screening and Diagnostics
- Assisted Reproductive Technology and Twin Pregnancy
- CRISPR and Genetic Engineering
- Cancer Immunotherapy and Biomarkers
Vita-Salute San Raffaele University
2020-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2020-2025
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2020-2025
Instituto de Investigación Sanitaria La Fe
2024
San Raffaele University of Rome
2022
University of North Carolina at Charlotte
2011
SoyMeds (United States)
2011
Kupffer cells (KCs) are highly abundant, intravascular, liver-resident macrophages known for their scavenger and phagocytic functions. KCs can also present antigens to CD8+ T promote either tolerance or effector differentiation, but the mechanisms underlying these discrepant outcomes poorly understood. Here, we used a mouse model of hepatitis B virus (HBV) infection, in which HBV-specific naive recognizing hepatocellular driven into state immune dysfunction, identify subset (referred as KC2)...
The development of a tractable small animal model faithfully reproducing human coronavirus disease 2019 pathogenesis would arguably meet pressing need in biomedical research. Thus far, most investigators have used transgenic mice expressing the ACE2 epithelial cells (K18-hACE2 mice) that are intranasally instilled with liquid severe acute respiratory syndrome 2 (SARS-CoV-2) suspension under deep anesthesia. Unfortunately, this experimental approach results disproportionate high central...
Reversing CD8+ T cell dysfunction is crucial in treating chronic hepatitis B virus (HBV) infection, yet specific molecular targets remain unclear. Our study analyzed co-signaling receptors during hepatocellular priming and traced the trajectory fate of dysfunctional HBV-specific cells. Early on, these cells upregulate PD-1, CTLA-4, LAG-3, OX40, 4-1BB, ICOS. While blocking co-inhibitory had minimal effect, activating 4-1BB OX40 converted them into antiviral effectors. Prolonged stimulation...
CD4+ T cells play a critical role in supporting antiviral humoral and cellular immune responses. Although these responses often coexist, one can sometimes dominate at the expense of other. For instance, lymphocytic choriomeningitis virus (LCMV) induces strong response but generates suboptimal neutralizing antibody (nAb) response, hindering viral clearance enabling persistent infection. Recent findings indicate that this skewed preference toward immunity is determined already during...
Abstract CD4 + T cells play a critical role in antiviral humoral and cellular immune responses. We have previously reported that subcutaneous lymphocytic choriomeningitis virus (s.c. LCMV) infection is characterized by stark compartmentalization of cells, leading to strong H 1 cell polarization but virtually absent follicular helper (T FH ) key drivers immunity. Here, we investigate the mechanisms responsible for this impaired differentiation. show T-bet induced LCMV encompass subset...
Abstract T DC are hematopoietic cells that combine dendritic cell (DC) and conventional T‐cell markers functional properties. They were identified in secondary lymphoid organs (SLOs) of naïve mice as expressing CD11c, major histocompatibility molecules (MHC)‐II, the receptor (TCR). Despite thorough characterization, a physiological role for remains to be determined. Unfortunately, using CD11c marker has caveat its upregulation on different cells, including upon activation. Here, we took...
Abstract Study question Can a deep learning tool accurately predict blastocyst development from single static oocyte images across diverse patient populations? Summary answer The automatic score was directly correlated with the formation rate in conventional treatments using oocytes and egg donation program. What is known already implementation of artificial intelligence (AI)-based algorithms has revolutionized gamete embryo evaluation assisted reproduction, providing standardized basis for...
The generation of effector CD8+ T cells (TEFF) requires activation naive (TN) by dendritic (DCs) within lymphoid tissue. To date, it remains elusive how the duration TN-DC interactions and integration signals are controlled in vivo. Here, we report that stroma-secreted ligands for CCR7 constrained interaction gradually inducing cell release from DCs. At late time points interactions, repositioned F-actin-promoting factor DOCK2 away DC interface to enable detachment, proliferation onset...
Abstract The development of a tractable small animal model faithfully reproducing human COVID-19 pathogenesis would arguably meet pressing need in biomedical research. Thus far, most investigators have used transgenic mice expressing the ACE2 epithelial cells (K18-hACE2 mice) that are intranasally instilled with liquid SARS-CoV-2 suspension under deep anesthesia. Unfortunately, this experimental approach results disproportionate high CNS infection leading to fatal encephalitis, which is...
Abstract T DC are hematopoietic cells that combine dendritic cell (DC) and conventional markers functional properties. They were identified in secondary lymphoid organs (SLOs) of naïve mice as expressing CD11c, major histocompatibility molecule (MHC)-II, the receptor (TCR) β chain. Despite thorough characterization to their potential properties, a physiological role for remains be determined. Unfortunately, using CD11c marker has caveat its upregulation on different cells, including upon...
Abstract Although humoral and cellular immunity upon viral infections usually co-exist, sometimes one of the two responses emerges as dominant is responsible for most antiviral activity. For example, vesicular stomatitis virus (VSV) infection induces early potent neutralizing antibody (nAb) responses, whereas lymphocytic choriomeningitis (LCMV) strong but weak nAb responses. Recent work from our laboratory has shown that unbalance observed also at level CD4 T cells with subcutaneous VSV...
Transfer of blastocysts with mosaic aneuploidies (TBMA) at pre-implantation genetic testing (PGT) is accepted as a “rescue” option in couples without euploid embryos, good neonatal outcomes are reported. No evidence exists regarding first trimester combined screening after TBMA, despite alterations fetal or placental development might be expected based on trophectoderm mosaicism. Pregnancy follow-up was performed and cases/controls confirmed fetal/neonatal abnormalities were excluded from...
Abstract Although humoral and cellular immunity upon viral infections usually co-exist, sometimes one of the two responses emerges is responsible for most antiviral activity. For example, vescicular stomatitis virus (VSV) infection induces early potent neutralizing antibody (nAb) responses, whereas lymphocytic choriomeningitis (LCMV) strong but weak nAb responses. Recent work from our laboratory has shown that unbalance observed also at level CD4 T cells with VSV inducing TFH polarization...