A5014798752- Immune Cell Function and Interaction
- Hepatitis B Virus Studies
- Immune cells in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Liver Disease Diagnosis and Treatment
- Atherosclerosis and Cardiovascular Diseases
- Advanced biosensing and bioanalysis techniques
- HER2/EGFR in Cancer Research
- Cancer Research and Treatments
- Chemotherapy-induced cardiotoxicity and mitigation
- Chemokine receptors and signaling
- Graphene and Nanomaterials Applications
- Cancer Treatment and Pharmacology
- Diabetic Foot Ulcer Assessment and Management
- Hepatitis Viruses Studies and Epidemiology
- Wound Healing and Treatments
- Hepatitis C virus research
- IL-33, ST2, and ILC Pathways
- Nanoparticle-Based Drug Delivery
- Renal Transplantation Outcomes and Treatments
- Liver physiology and pathology
Vita-Salute San Raffaele University
2019-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2019-2024
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2019-2024
Inserm
2013-2023
Université Sorbonne Paris Nord
2023
San Raffaele University of Rome
2023
Sorbonne Université
2018-2023
Université Paris Cité
2014-2023
Laboratoire de Recherche Vasculaire Translationnelle
2014-2023
University of Milan
2020
Kupffer cells (KCs) are highly abundant, intravascular, liver-resident macrophages known for their scavenger and phagocytic functions. KCs can also present antigens to CD8+ T promote either tolerance or effector differentiation, but the mechanisms underlying these discrepant outcomes poorly understood. Here, we used a mouse model of hepatitis B virus (HBV) infection, in which HBV-specific naive recognizing hepatocellular driven into state immune dysfunction, identify subset (referred as KC2)...
Background— The atheromodulating activity of B cells during the development atherosclerosis is well documented, but mechanisms by which these are regulated have not been investigated. Methods and Results— Here, we analyzed contribution Qa-1–restricted CD8 + regulatory T to control follicular helper–germinal center B-cell axis atherogenesis. Genetic disruption cell function in atherosclerosis-prone apolipoprotein E knockout mice resulted overactivation this secondary lymphoid organs, led...
The liver's unique characteristics have a profound impact on the priming and maintenance of adaptive immunity. This review delves into cellular circuits that regulate immune responses in liver, with specific focus hepatitis B virus infection as an illustrative example. A key aspect highlighted is specialized role CD8+ T cells, leading to distinct state hyporesponsiveness. Additionally, influence hemodynamics anatomical features, particularly during liver fibrosis cirrhosis, differentiation...
CD8 + T cells are key antiviral effectors against hepatitis B virus (HBV), yet their number and function can be compromised in chronic infections. Preclinical HBV models displaying cell dysfunction showed that interleukin-2 (IL-2)–based treatment, unlike programmed death ligand 1 (PD-L1) checkpoint blockade, could reverse this defect, suggesting its therapeutic potential HBV. However, IL-2’s effectiveness is hindered by pleiotropic nature, because receptor found on various immune cells,...
Reversing CD8+ T cell dysfunction is crucial in treating chronic hepatitis B virus (HBV) infection, yet specific molecular targets remain unclear. Our study analyzed co-signaling receptors during hepatocellular priming and traced the trajectory fate of dysfunctional HBV-specific cells. Early on, these cells upregulate PD-1, CTLA-4, LAG-3, OX40, 4-1BB, ICOS. While blocking co-inhibitory had minimal effect, activating 4-1BB OX40 converted them into antiviral effectors. Prolonged stimulation...
Group 1 innate lymphoid cells (ILCs), which comprise both natural killer (NK) and ILC1s, are important effectors that can also positively negatively influence adaptive immune responses. The latter function is generally ascribed to the ability of NK recognize kill activated T cells. Here, we used multiphoton intravital microscopy in mouse models hepatitis B study intrahepatic behavior group ILCs their cross-talk with virus (HBV)–specific CD8 + We found hepatocellular antigen recognition by...
Humoral rejection is the most common cause of solid organ transplant failure. Here, we evaluated a cohort 49 patients who were successfully grafted with allogenic islets and determined that appearance donor-specific anti-HLA antibodies (DSAs) did not accelerate rate islet graft attrition, suggesting resistance to humoral rejection. Murine DSAs bound allogeneic targets expressed by cells induced their destruction in vitro; however, passive transfer same affect survival murine models. Live...
In control: The ligand positioning and orientation on multifunctional nanoparticles (MNP) are controlled by using a versatile bimodular approach that exploits an 11 amino acid (U11) homing peptide genetically fused with haloalkane dehalogenase (HALO) enzyme. resultant have good targeting efficiency selectivity toward broadly occurring urokinase plasminogen activator receptor positive human cancer cells. Multifunctional (MNPs) combine unique superparamagnetic properties fluorescence emission...
Background Experimental atherosclerosis is characterized by the formation of tertiary lymphoid structures (TLOs) within adventitial layer, which involves chemokine-expressing aortic smooth muscle cells (SMCs). TLOs have also been described around human atherothrombotic arteries but mechanisms their remain poorly investigated. Herein, we tested whether vascular SMCs play role that would trigger in arteries. Results We first characterized, flow cytometry and immunofluorescence analysis,...
Effective neutrophil migration to sites of inflammation is crucial for host immunity. A coordinated cascade steps allows intravascular leukocytes counteract the shear stress, transmigrate through endothelial layer, and move toward extravascular, static environment. Those events are tightly orchestrated by integrins, but, while molecular mechanisms leading their activation have been characterized, regulatory pathways promoting detachment remain elusive. In light this, it has long known that...
Here, we provide detailed protocols for the isolation of mouse Kupffer cells - liver-resident macrophages phenotypic (e.g., via flow cytometry, mass or RNA-sequencing) analyses functional experiments involving cell culture. The procedures presented can be adapted other hepatic populations. For complete details on use and execution this protocol, please refer to De Simone et al. (2021).
Neoadjuvant chemotherapy has been established as the standard of care for HER2-positive breast cancer since it allows down-staging, up to pathological complete response. The in neoadjuvant setting is a combination highly cytotoxic drugs such anthracyclines and anti-HER2 monoclonal antibody. Despite this cocktail response 50%, their co-administration strongly limited by intrinsic cardiotoxicity. Therefore, only sequential administration treatment allowed. Here, we propose anthracycline...
Aim To investigate whether plasma concentrations of proprotein‐convertase‐subtilisin/kexin type 9 (PCSK9) were associated with cardiovascular (CV) events in two cohorts patients 2 diabetes mellitus. Methods We considered from the DIABHYCAR ( n = 3137) and SURDIAGENE 1468) studies. Baseline PCSK9 concentration was measured using an immunofluorescence assay. In post hoc, but preplanned, analyses we assessed relationship between following endpoints: (1) a combined endpoint major CV events:...
Abstract Despite the advent of numerous targeted therapies in clinical practice, anthracyclines, including doxorubicin (DOX), continue to play a pivotal role breast cancer (BC) treatment. DOX directly disrupts DNA replication, demonstrating remarkable efficacy against BC cells. However, its non-specificity toward cells leads significant side effects, limiting utility. Interestingly, can also enhance antitumor immune response by promoting immunogenic cell death cells, thereby facilitating...
Abstract There is a need for new targets to specifically localize inflammatory foci, usable in wide range of organs. Here, we hypothesized that the cleaved molecular form CD31 suitable target imaging inflammation. We evaluated bioconjugate D-P8RI, synthetic peptide binds all cells with CD31, an experimental rat model sterile acute Male Wistar rats were injected turpentine oil into gastrocnemius muscle two days before 99m Tc-HYNIC-D-P8RI (or its analogue L-Proline) SPECT/CT or [ 18 F]FDG...
Abstract Systemic chemotherapy has not significantly reduced clinical demand for triple negative breast cancer (TNBC) treatments. To address the need more effective therapy, use of nonviral nanoparticles is explored to deliver suicide gene therapy as valuable alternative protect nucleic acids in bloodstream and improve their tumor uptake. Biocompatible cationic lipid are developed a novel delivery system plasmid encoding saporin. Active targeting accomplished by taking advantage nanoparticle...