A5074172438- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune cells in cancer
- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Antibiotic Resistance in Bacteria
- Cellular transport and secretion
- Glycosylation and Glycoproteins Research
- Antimicrobial Resistance in Staphylococcus
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Bariatric Surgery and Outcomes
- Signaling Pathways in Disease
- Bacterial Identification and Susceptibility Testing
- Exercise and Physiological Responses
- Nanoparticle-Based Drug Delivery
- Electrospun Nanofibers in Biomedical Applications
- RNA modifications and cancer
- RNA regulation and disease
- Sleep and Wakefulness Research
- Infective Endocarditis Diagnosis and Management
- Immune Response and Inflammation
- Neurological diseases and metabolism
- Diet and metabolism studies
Centre National de la Recherche Scientifique
2021-2023
Centre de Recherche sur l'Inflammation
2020-2023
Inserm
2010-2023
Université Paris Cité
2010-2023
Délégation Paris 7
2018-2022
Sorbonne Paris Cité
2018-2022
Babraham Institute
2022
Hôpital Beaujon
2022
Hôpital Bichat-Claude-Bernard
2012
Université Claude Bernard Lyon 1
2012
Interleukin 2 (IL-2) is a key homeostatic cytokine, with therapeutic applications in both immunogenic and tolerogenic immune modulation. Clinical use has been hampered by pleiotropic functionality widespread receptor expression, unexpected adverse events. Here, we developed novel mouse strain to divert IL-2 production, allowing identification of contextual outcomes. Network analysis identified priority access for Tregs competitive fitness cost production among conventional CD4 T cells. CD8...
Abstract Glucagon-Like Peptide-1 (GLP-1) undergoes rapid inactivation by dipeptidyl peptidase-4 (DPP4) suggesting that target receptors may be activated locally produced GLP-1. Here we describe GLP-1 positive cells in the rat and human stomach found these co-expressing ghrelin or somatostatin able to secrete active rats. In lean rats, a gastric load of glucose induces parallel rise levels both portal veins. This was abrogated HFD obese rats but restored after vertical sleeve gastrectomy...
T cell receptor (TCR) activation is modulated by mechanisms such as TCR endocytosis, which thought to terminate signalling. Here we show that, upon internalization, continues signal from a set of specialized endosomes that are crucial for functions. Mechanistically, ligation leads clathrin-mediated internalization the TCR-CD3ζ complex, while maintaining CD3ζ signalling, in endosomal vesicles contain insulin responsive aminopeptidase (IRAP) and SNARE protein Syntaxin 6. Destabilization this...
Recent evidences indicate an important role of tissue inflammatory responses by innate immune cells in allograft acceptance and survival. Here we investigated the mast (MC) acute male to female skin rejection model using red MC basophil (RMB) mice enabling conditional depletion. Kinetic analysis showed that MCs markedly accelerate rejection. They induced early response through degranulation boosted local synthesis KC, MIP-2 TNF. This enhanced neutrophil infiltration compared a female-female...
Cell surface receptor internalization can either terminate signaling or activate alternative endosomal pathways. We investigated here whether is involved in the function of human receptors for Fc immunoglobulin fragments (FcRs): FcαRI, FcγRIIA, and FcγRI. All these were internalized after their cross-linking with receptor-specific antibodies, but intracellular trafficking was different. FcαRI targeted directly to lysosomes, while FcγRIIA FcγRI particular compartments described by insulin...
Effective neutrophil migration to sites of inflammation is crucial for host immunity. A coordinated cascade steps allows intravascular leukocytes counteract the shear stress, transmigrate through endothelial layer, and move toward extravascular, static environment. Those events are tightly orchestrated by integrins, but, while molecular mechanisms leading their activation have been characterized, regulatory pathways promoting detachment remain elusive. In light this, it has long known that...
Electrostimulation (ES) can be defined as a safe physical method to induce stem cell differentiation.The aim of this study is evaluate the effectiveness ES on bone marrow mesenchymal cells (BMSCs) seeded in collagen scaffolds terms proliferation and differentiation into cardiomyocytes.BMSCs were isolated from Wistar rats 3D type 1 templates measuring 25 x 6 mm.Bipolar vitro was performed during 21 days.Electrical impedance measured.Expression cardiac markers assessed by...
ABSTRACT The mechanisms of antibiotic-induced cell death are poorly understood despite the critical role bactericidal activities antibiotics for successful treatment severe infections. These include irreversible damaging macromolecules by reactive oxygen species and bacteriolysis mediated peptidoglycan hydrolases (autolysins). We have assessed contribution second mechanism using an autolysin-deficient mutant Enterococcus faecalis shown that it contributes to amoxicillin-induced lysis only at...
Human disc-large homolog (hDlg), also known as synapse-associated protein 97, is a scaffold protein, member of the membrane-associated guanylate kinase family, implicated in neuronal synapses and epithelial-epithelial cell junctions whose expression function remains poorly characterized most tissues, particularly vasculature. In human vascular hDlg highly expressed smooth muscle cells (VSMCs). Using yeast two-hybrid system to screen aorta cDNA library, we identified mitogen-activated...
Flow cytometry and confocal microscopy were used to study the effects of vancomycin, daptomycin, telavancin, PA1409, a new investigational vancomyquine, on morphology, membrane potential, permeability glycopeptide-susceptible -resistant Enterococcus faecalis strains. Daptomycin exerted most pronounced bacterial size against susceptible resistant
Abstract Fluorescence confocal microscopy is commonly used to analyze the regulation membrane proteins expression such as G protein‐coupled receptors (GPCRs). With this approach, internal movement of GPCRs within cell can be observed with a high degree resolution. However, these techniques led complex and time‐consuming analysis did not allow large population events sampled. A recent approach termed imaging flow cytometry (IFC), which combines fluorescence microscopy, had two main advantages...
Abstract Although endocytosis of cell surface receptors is generally thought to terminate the signaling, for some receptors, sustains signaling. We wondered if endosomal signaling participates function Fc immunoglobulin fragments (FcRs) that are highly internalized after their activation. demonstrate here four different FcRs follow distinct endocytic pathways While FcαRI into lysosomes, FcγRIIA and partially retained in early endosomes, whereas inhibitory receptor FcγRIIB endosomes decorated...
Effective neutrophil migration to sites of inflammation is crucial for host immunity. A coordinated cascade steps allows intravascular leukocytes counteract the shear stress, transmigrate through endothelial layer and move towards extravascular, static environment. Those events are tightly orchestrated by integrins but, while molecular mechanisms leading their activation have been characterized, regulatory pathways promoting detachment remain elusive. In light this, it has long known that...