A5022372742- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- Cellular Mechanics and Interactions
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- Chronic Lymphocytic Leukemia Research
- CAR-T cell therapy research
- Calcium signaling and nucleotide metabolism
- Cytomegalovirus and herpesvirus research
- Galectins and Cancer Biology
- Cellular transport and secretion
- Immune Response and Inflammation
- Single-cell and spatial transcriptomics
- Force Microscopy Techniques and Applications
- Immune cells in cancer
- NF-κB Signaling Pathways
- Ubiquitin and proteasome pathways
- Neuroinflammation and Neurodegeneration Mechanisms
- HIV Research and Treatment
- Lymphoma Diagnosis and Treatment
- Signaling Pathways in Disease
- Diabetes and associated disorders
Institut Curie
2015-2024
Inserm
2015-2024
Université Paris Sciences et Lettres
2015-2024
Immunité et Cancer
2010-2024
Centre d’Immunologie de Marseille-Luminy
2016
Institut Necker Enfants Malades
1995-2009
Hôpital Necker-Enfants Malades
1993-1998
Washington University in St. Louis
1997
Institut Pasteur
1994
Hôpital des Enfants
1993
Fas (also known as Apo1 and CD95) is a cell surface receptor involved in apoptotic death. expression function were analyzed three children (including two siblings) with lymphoproliferative syndrome, of whom also had autoimmune disorders. A large deletion the gene encoding no detectable characterized most affected patient. Clinical manifestations related patients less severe: Fas-mediated apoptosis was impaired within intracytoplasmic domain detected. These findings illustrate crucial...
Abstract We show in this study that human T cells purified from peripheral blood, cell clones, and Jurkat release microvesicles the culture medium. These have a diameter of 50–100 nm, are delimited by lipidic bilayer membrane, bear TCR β, CD3ε, ζ. This microvesicle production is regulated because it highly increased upon activation, whereas another mitogenic signal, such as PMA ionomycin, does not induce any release. cell-derived also contain tetraspan protein CD63, suggesting they originate...
A mutation in ORAI1, the gene encoding pore-forming subunit of Ca(2+)-release-activated Ca(2+) (CRAC) channel, abrogates store-operated entry into cells and impairs lymphocyte activation. Stromal interaction molecule 1 (STIM1) endoplasmic reticulum activates ORAI1-CRAC channels. We report on three siblings from one kindred with a clinical syndrome immunodeficiency, hepatosplenomegaly, autoimmune hemolytic anemia, thrombocytopenia, muscular hypotonia, defective enamel dentition. Two these...
Complete interferon-gamma receptor 1 (IFNgammaR1) deficiency has been identified previously as a cause of fatal bacillus Calmette-Guérin (BCG) infection with lepromatoid granulomas, and disseminated nontuberculous mycobacterial (NTM) in children who had not inoculated BCG. We report here kindred partial IFNgammaR1 deficiency: one child afflicted by BCG tuberculoid sibling, BCG, clinical tuberculosis. Both responded to antimicrobials are currently well without prophylactic therapy. Impaired...
Mucosal associated invariant T cells (MAIT) are innate lymphocytes that detect a large variety of bacteria and yeasts. This recognition depends on the detection microbial compounds presented by evolutionarily conserved major-histocompatibility-complex (MHC) class I molecule, MR1. Here we show MAIT display cytotoxic activity towards MR1 overexpressing non-hematopoietic cocultured with bacteria. The NK receptor, CD161, highly expressed cells, modulated cytokine but not response triggered...
// Asma Beldi-Ferchiou 1, 2, 11 , Marion Lambert 2 Stéphanie Dogniaux 3 Frédéric Vély 4, 5 Eric Vivier Daniel Olive 6 Dupuy 1 Frank Levasseur David Zucman 7 Céleste Lebbé 8 Damien Sène 9 Claire Hivroz 4 Sophie Caillat-Zucman 10 Institut National de Recherche Médicale (INSERM) UMR1149, Centre Sur l’Inflammation, Équipe Immunité Innée Chez l’enfant, Hôpital Robert Debré, Paris, France...
T cells are mechanosensitive but the effect of stiffness on their functions is still debated. We characterize herein how human primary CD4+ cell affected by within physiological Young’s modulus range 0.5 kPa to 100 kPa. Stiffness modulates lymphocyte migration and morphological changes induced TCR/CD3 triggering. also increases TCR-induced immune system, metabolism cell-cycle-related genes. Yet, upon stimulation, while cytokine production a wide stiffness, from hundreds Pa kPa, metabolic...
Stimulation of antigen receptors lymphocytes triggers a transitory release Ca2+ from internal stores and the opening transmembrane conductive pathway. The latter underlies sustained increase intracellular free calcium concentration, it seems to be key event in Ca(2+)-dependent biochemical cascade leading T cell proliferation. Alternatively, pharmacological depletion by itself activates influx. This has led hypothesis that antigen-triggered influx is secondary stores. However, precise...
T cells are major players of adaptive immune response in mammals. Recognition an antigenic peptide association with the histocompatibility complex at surface antigen presenting cell (APC) is a specific and sensitive process whose mechanism not fully understood. The potential contribution mechanical forces activation increasingly debated, although these scarcely defined hold only limited experimental evidence. In this work, we have implemented biomembrane force probe (BFP) setup model APC to...
Abstract Regulatory T cells (Tregs) are plastic playing a pivotal role in the maintenance of immune homeostasis. Tregs actively adapt to microenvironment where they reside; as consequence, their molecular and functional profiles differ among tissues pathologies. In tumors, features acquired by remains poorly characterized. Here, we observe that human tumor-infiltrating selectively overexpress CD74, MHC class II invariant chain. CD74 has been previously described regulator antigen-presenting...
Abstract The initiation of adaptive immune responses requires the direct interaction dendritic cells (DCs) with naive T lymphocytes. It is well established that maturation state DCs has a critical impact on outcome response. We show here mature form stable conjugates and induce formation organized synapses. Immature DCs, in contrast, few no A dynamic analysis revealed can long-lasting interactions cells, even absence Ag. only short intermittent contacts, suggesting premature termination...
A four-month-old boy with primary immunodeficiency was found to have a homozygous germ-line mutation of the gene encoding CD3zeta subunit T-cell receptor-CD3 complex. is necessary for development and function T cells. Some patient's cells had low levels complex carried Q70X in both alleles CD3zeta, whereas other normal bore on only one allele plus three heterozygous somatic mutations allele, allowing expression poorly functional complexes.
Cytokine secretion by T lymphocytes plays a central role in mounting adaptive immune responses. However, little is known about how newly synthesized cytokines, once produced, are routed within cells and the mechanisms involved regulating their secretions. In this study, we investigated of cytoskeleton remodeling at immunological synapse (IS) cytokine secretion. We show that key regulator remodeling, Rho GTPase Cdc42, controls IFN-γ primary human CD4+ lymphocytes. Surprisingly, microtubule...
Complete lack of function the tyrosine kinase ZAP70 in humans results a severe immunodeficiency, characterized by mature CD8(+) T cells and non-functional CD4(+) cells. We report herein an immunodeficiency with inherited hypomorphic mutation due to single G-to-A substitution non-coding intron. This introduces new acceptor splice site allows low levels normal alternative splicing WT expression. partial deficiency compromised TCR signaling that was totally restored increased expression ZAP70,...