A5038903335- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- T-cell and Retrovirus Studies
- Animal Virus Infections Studies
- Vector-Borne Animal Diseases
- Immune Cell Function and Interaction
- Cystic Fibrosis Research Advances
- Animal Disease Management and Epidemiology
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Chemokine receptors and signaling
- Pneumocystis jirovecii pneumonia detection and treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antimicrobial Peptides and Activities
- Helminth infection and control
- Cancer Immunotherapy and Biomarkers
- Bacterial Genetics and Biotechnology
- Asthma and respiratory diseases
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Phagocytosis and Immune Regulation
- Antifungal resistance and susceptibility
- Neonatal Respiratory Health Research
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2016-2024
Vita-Salute San Raffaele University
2015-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2024
University of California, San Francisco
2020-2024
Nurix (United States)
2021-2023
San Raffaele University of Rome
2021
IRCCS Ospedale San Raffaele
2021
University of Liverpool
2019
University of Perugia
1975-1987
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
1983-1984
Platelets have a wide range of functions including critical roles in hemostasis, thrombosis, and immunity. We hypothesized that during acute inflammation, such as life-threatening sepsis, there are fundamental changes the sites platelet production phenotypes resultant platelets. Here, we showed sepsis spleen was major site megakaryopoiesis production. Sepsis provoked an adrenergic-dependent mobilization megakaryocyte-erythrocyte progenitors (MEPs) from bone marrow to spleen, where IL-3...
Reversing CD8+ T cell dysfunction is crucial in treating chronic hepatitis B virus (HBV) infection, yet specific molecular targets remain unclear. Our study analyzed co-signaling receptors during hepatocellular priming and traced the trajectory fate of dysfunctional HBV-specific cells. Early on, these cells upregulate PD-1, CTLA-4, LAG-3, OX40, 4-1BB, ICOS. While blocking co-inhibitory had minimal effect, activating 4-1BB OX40 converted them into antiviral effectors. Prolonged stimulation...
Cystic fibrosis (CF) is a genetic disorder affecting several organs including airways. Bacterial infection, inflammation and iron dysbalance play major role in the chronicity severity of lung pathology. The aim this study was to investigate effect lactoferrin (Lf), multifunctional iron-chelating glycoprotein innate immunity, CF murine model Pseudomonas aeruginosa chronic infection. To induce C57BL/6 mice, either cystic transmembrane conductance regulator (CFTR)-deficient...
Lactoferrin (Lf), an iron-chelating glycoprotein of innate immunity, produced by exocrine glands and neutrophils in infection/inflammation sites, is one the most abundant defence molecules airway secretions. Lf, a pleiotropic molecule, exhibits antibacterial anti-inflammatory functions. These properties may play relevant role infections characterized exaggerated inflammatory response, as Pseudomonas aeruginosa lung infection cystic fibrosis (CF) subjects. To verify Lf infection, we evaluated...
Cystic fibrosis (CF) lung disease is characterized by an inflammatory response that can lead to terminal respiratory failure. The cystic transmembrane conductance regulator (CFTR) mutated in CF, and we hypothesized dysfunctional CFTR platelets, which are key participants immune responses, a central determinant of CF inflammation. We found deletion platelets produced exaggerated acute inflammation platelet activation after intratracheal LPS or Pseudomonas aeruginosa challenge. loss function...
Mitochondrial therapy offers an alternative strategy for attenuation of hyperinflammation in cystic fibrosis lung disease.
The clinical development of antibiotics with a new mode action combined efficient pulmonary drug delivery is priority against untreatable Pseudomonas aeruginosa lung infections. POL7001 macrocycle antibiotic belonging to the novel class protein epitope mimetic (PEM) molecules selective and potent activity P. We investigated ventilator-associated pneumonia (VAP) cystic fibrosis (CF) as indications potential treat MICs comparators were measured for reference strains. therapeutic efficacy given...
Chronic infection by Pseudomonas aeruginosa in cystic fibrosis (CF) patients is a major contributor to progressive lung damage and poorly treated available antibiotic therapy. An alternative approach the development of additional treatments identify complementary therapies which target bacterial virulence factors necessary for establishment and/or maintenance chronic infection. The P. elastase (LasB) has been suggested as an attractive anti-virulence due its extracellular location, harmful...
Abstract The Gram-negative bacterium Pseudomonas aeruginosa represents a prototype of multi-drug resistant opportunistic pathogens for which novel therapeutic options are urgently required. In order to identify new candidates as potential drug targets, we combined large-scale transposon mutagenesis data analysis and bioinformatics predictions retrieve set putative essential genes conserved in P. predicted encode cell envelope or secreted proteins. By generating unmarked deletion conditional...
Antibiotic discovery and preclinical testing are needed to combat the Pseudomonas aeruginosa health threat. Most frequently, antibiotic efficacy is tested in models of acute respiratory infection, with chronic pneumonia remaining largely unexplored. This approach generates serious concerns about evaluation treatment for chronically infected patients, highlights need animal that mimic course human disease. In this study, marketed antibacterial drugs tobramycin (TOB) colistin (COL) was murine...
Neutrophilic inflammation is a key determinant of cystic fibrosis (CF) lung disease. Neutrophil-derived free DNA, released in the form extracellular traps (NETs), significantly correlates with impaired function patients CF, underlying their pathogenetic role CF Thus, specific approaches to control NETosis neutrophils migrated into lungs may be clinically relevant CF. We investigated efficacy phosphodiesterase (PDE) type-4 inhibitors, vitro , on NET release by from healthy volunteers and...
Chronic granulomatous disease (CGD) is a primary immunodeficiency due to deficiency in one of the subunits nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex. CGD patients are characterized by an increased susceptibility bacterial and fungal infections, granuloma formation excessive inflammatory responses. Several gene therapy approaches with lentiviral vectors have been proposed but there lack vivo data on ability control infections inflammation. We set up mouse model acute...
Pseudomonas aeruginosa is one of the most critical multidrug-resistant opportunistic pathogens in humans, able to cause both lethal acute and chronic lung infections. Thorough knowledge regulatory mechanisms involved establishment persistence airways infections by P. remains elusive. Emerging candidates as molecular regulators pathogenesis are small RNAs, which act posttranscriptionally signal transducers host cues. Known for being regulation biofilm formation responsive envelope stress...
Despite intensive antimicrobial and anti-inflammatory therapies, Cystic Fibrosis (CF) patients are subjected to chronic infections due opportunistic pathogens, including multi-drug resistant (MDR) Pseudomonas aeruginosa. Macrophages from CF show many evidences of reduced phagocytosis in terms internalisation capability, phagosome maturation, intracellular bacterial killing. In this study, we investigated if apoptotic body-like liposomes (ABL) loaded with phosphatidylinositol 5-phosphate...
SUMMARY A study was carried out to determine whether bovid herpesvirus-2 ( bhv -2) is able induce a recurrent infection in experimentally infected calves. Twelve calves with the virus were treated dexamethasone dms ) beginning 69 days after infection, ie, several weeks animals had recovered from disease and negative for -2. The stress induced by treatment failed reactivate clinical condition or shedding of However, reactivated latent infectious bovine rhinotracheitis ibr all previously...
SUMMARY The behavior of Bovid herpesvirus 2 in the skin, lymph nodes, and nervous system calves injected intravenously with virus was studied. After a latent phase that lasted up to 40 hours after inoculation, produced systemic infection which characterized by primary localization system. Between postinoculation days 7 12, tended persist skin only. finding intranuclear inclusions pyramidal cells cerebral cortex neurons superior cervical stellate ganglia supports hypothesis might utilize...
<h3>Background</h3> The E3 ubiquitin ligase Casitas B-lineage lymphoma B (CBL-B) is expressed in leukocytes and regulates signaling pathways T NK cells, significantly limiting their antitumor effector function. In cells CBL-B attenuates activation initiated by TCR engagement, part mediating the requirement for CD28 co-stimulation, thus setting threshold cell activation. functions downstream of TAM receptors negatively cytokine production cytotoxicity. <h3>Methods</h3> Here we describe...