A5031374187- Trace Elements in Health
- Iron Metabolism and Disorders
- Infant Nutrition and Health
- Hemoglobinopathies and Related Disorders
- Nitric Oxide and Endothelin Effects
- Hemoglobin structure and function
- Enzyme-mediated dye degradation
- Corrosion Behavior and Inhibition
- Child Nutrition and Water Access
- Marine Invertebrate Physiology and Ecology
- Metal-Catalyzed Oxygenation Mechanisms
- Metal complexes synthesis and properties
- Marine Toxins and Detection Methods
- Enzyme Structure and Function
- Electrochemical Analysis and Applications
- DNA and Nucleic Acid Chemistry
- Porphyrin Metabolism and Disorders
- Protein Interaction Studies and Fluorescence Analysis
- Pediatric Hepatobiliary Diseases and Treatments
- Photosynthetic Processes and Mechanisms
- Glycosylation and Glycoproteins Research
- Sulfur Compounds in Biology
- Fungal and yeast genetics research
- Meat and Animal Product Quality
- Electron Spin Resonance Studies
University of Molise
2015-2025
Ospedale L. Bonomo
2024
University of Foggia
2017-2022
IRCCS San Camillo Hospital
2021
Azienda Sanitaria Unità Locale di Reggio Emilia
2019-2020
Istituti di Ricovero e Cura a Carattere Scientifico
2020
Roma Tre University
2008-2012
University of Messina
1996-2006
University of Utah
2005
University of California, Los Angeles
2005
Ferroportin (Fpn) is the only known iron exporter in vertebrates. Hepcidin, a peptide secreted by liver response to or inflammation, binds Fpn, inducing its internalization and degradation. We show that after binding of hepcidin, Fpn tyrosine phosphorylated at plasma membrane. Mutants human do not get internalized are slowly either absent impaired phosphorylation. identify adjacent tyrosines as phosphorylation sites mutation both prevents hepcidin-mediated internalization. Once internalized,...
Mutations in the iron exporter ferroportin (Fpn) (IREG1, SLC40A1 , and MTP1) result hemochromatosis type IV, a disorder with dominant genetic pattern of inheritance heterogeneous clinical presentation. Most patients develop loading Kupffer cells relatively low saturation plasma transferrin, but others present high transferrin iron-loaded hepatocytes. We show that known human mutations introduced into mouse Fpn-GFP generate proteins either are defective cell surface localization or have...
Here, we show that extracorporeal shock waves (ESW), at a low energy density value, quickly increase neuronal nitric oxide synthase (nNOS) activity and basal (NO) production in the rat glioma cell line C6. In addition, treatment of C6 cells with ESW reverts decrease nNOS NO induced by mixture lipopolysaccharides (LPS), interferon‐γ (IFN‐γ) plus tumour necrosis factor‐α (TNF‐α). Finally, efficiently downregulates NF‐κB activation NF‐κB‐dependent gene expression, including inducible NOS TNF‐α....
The evidence that nitric oxide (NO) production is possible by a non-enzymatic pathway has already been shown under restrictive experimental conditions. Here we show NO can non-enzymatically be formed with short-time kinetics (min), 'bombing' shock waves solution containing 1 mM hydrogen peroxide and 10 L-arginine. This procedure widening its medical application surprisingly positive effects in tissue regeneration our finding could one of the first steps for understanding biochemical...
Human lactoferrin (hLf), an 80 kDa multifunctional iron binding cationic glycoprotein, is constitutively secreted by exocrine glands and neutrophils during inflammation. HLf recognized as a key element in the host immune defense system. The vitro vivo experiments are carried out with bovine Lf (bLf), which shares high sequence homology identical functions hLf including anti-inflammatory activity. Here, 'pure' M1 human macrophages, obtained stimulation mixture of 10 pg/ml LPS 20 ng/ml IFN-γ,...
Cystic fibrosis (CF) is a genetic disorder affecting several organs including airways. Bacterial infection, inflammation and iron dysbalance play major role in the chronicity severity of lung pathology. The aim this study was to investigate effect lactoferrin (Lf), multifunctional iron-chelating glycoprotein innate immunity, CF murine model Pseudomonas aeruginosa chronic infection. To induce C57BL/6 mice, either cystic transmembrane conductance regulator (CFTR)-deficient...
Saccharomyces cerevisiae transcriptionally regulates the expression of plasma membrane high affinity iron transport system in response to need. This is comprised products FET3 and FTR1 genes. We show that Fet3p Ftr1p are post-translationally regulated by iron. Incubation cells leads internalization degradation both Ftr1p. Yeast strains defective endocytosis (Deltaend4) a reduced iron-induced loss Fet3p-Ftr1p. In with deletion vacuolar protease PEP4, medium accumulation vacuole. Iron-induced...
The reaction between reduced Pseudomonas nitrite reductase and has been studied by stopped-flow rapid-freezing EPR spectroscopy. interpretation of the kinetics at pH 8.0 is consistent with following mechanism (where k1 k3 much greater than k2). [formula: see text] bimolecular step (Step 1) very fast, being lost in dead time a rapid mixing apparatus; stoichiometry complex estimated to correspond one NO2- molecule/heme d1. final species fully enzyme NO bound heme d1; all concentrations...
Human plasma or serum from donors of age comprised between 15 and 95 years was analyzed for paramagnetic total copper content, as well immunoreactive ceruloplasmin content oxidase activity. All parameters were essentially unaltered, except the which increased 2-fold upon aging. A dramatic change electron resonance spectrum due to occurred in individuals over 65 old associated with both an increase type 1 signal intensity appearance new resonances a 2 species. Ceruloplasmin isolated either...
The binding of the fluorescent probe 4,4'-bis[1-(phenylamino)-8-naphthalenesulfonate] (bis-ANS) to bovine alpha-lactalbumin (alpha-LA) was investigated. A strong dependence Kd value with bound calcium stoichiometry found, values ranging from 6.2 +/- 0.4 64.6 5.9 microM for apo-alpha-LA and 1:1 Ca(II)-alpha-LA, respectively. 350-fold enhancement bis-ANS emission observed in protein-bis-ANS complex, along an approximately 33-nm blue shift. Both appeared be related hydrophobicity site were...
Abstract The reactivity with dioxygen of a mammalian (sheep) ceruloplasmin, anaerobically reduced ascorbate, was found to depend on the state Type 2 and 3 copper centers, as monitored by EPR optical spectroscopy. A complete reoxidation air after anaerobic reduction ascorbate observed samples (A) purified single-step procedure described for chicken ceruloplasmin (Calabrese, L., Carbonaro, M., Musci, G. (1988) J. Biol. Chem. 263, 6480-6483), while prepared traditional multistep (B) or...