A5059574953- Inflammatory Bowel Disease
- COVID-19 Clinical Research Studies
- Liver Disease Diagnosis and Treatment
- HIV Research and Treatment
- Mycobacterium research and diagnosis
- Prion Diseases and Protein Misfolding
- Dermatological and COVID-19 studies
- HER2/EGFR in Cancer Research
- Autoimmune and Inflammatory Disorders
- Neurological diseases and metabolism
- Pancreatitis Pathology and Treatment
- Medieval European Literature and History
- Long-Term Effects of COVID-19
- Microscopic Colitis
- Tuberculosis Research and Epidemiology
- Gastrointestinal Tumor Research and Treatment
- HIV-related health complications and treatments
- Hepatitis C virus research
- Diagnosis and treatment of tuberculosis
- Intraperitoneal and Appendiceal Malignancies
- Hepatitis B Virus Studies
- Immune Cell Function and Interaction
- Sperm and Testicular Function
- Eosinophilic Esophagitis
- Alcoholism and Thiamine Deficiency
Azienda Socio Sanitaria Territoriale Santi Paolo e Carlo
2023
Luigi Sacco Hospital
2013-2022
University of Milan
2012-2022
ASST Fatebenefratelli Sacco
2016-2022
Ospedale Papa Giovanni XXIII
2020
University of Milano-Bicocca
2019
Istituti Clinici Scientifici Maugeri
2019
Ospedale Maggiore
2012
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2012
Mylan (Switzerland)
2006
COVID-19 is characterised by respiratory symptoms, which deteriorate into failure in a substantial proportion of cases, requiring intensive care up to third patients admitted hospital. Analysis the pathological features lung tissues who have died with could help us understand disease pathogenesis and clinical outcomes.We systematically analysed tissue samples from 38 two hospitals northern Italy between Feb 29 March 24, 2020. The most representative areas identified at macroscopic...
SARS2-CoV-2 breakout in Italy caused a huge number of severely ill patients with serious increase mortality. Although lungs seem to be the main target infection, very few information are available about liver involvement, possibly evocating systemic disease. Post-mortem wedge biopsies from 48 died severe pulmonary COVID-19 disease respiratory failure were collected two hospitals northern Italy. No patient had clinical symptoms or signs before and during hospitalization; for each them...
ABSTRACT Importance The analysis of lung tissues patients with COVID-19 may help understand pathogenesis and clinical outcomes in this life-threatening respiratory illness. Objective To determine the histological patterns tissue severe COVID-19. Design participants Lungs 38 cases who died for two hospital Northern Italy were systematically analysed. Hematoxylin-eosin staining, immunohistochemistry inflammatory infiltrate cellular components, electron microscopy performed. Results features...
Vagal activity has protective effects in ischemic heart disease. We tested whether vagal stimulation (VS) could modulate the inflammatory reaction, a major determinant of cardiac injury after ischemia/reperfusion. Four groups male rats underwent myocardial ischemia (30 minutes) and reperfusion (24 hours). One group VS (40 minutes), 1 plus atrial pacing (VS + Pacing), nicotinic inhibition by mecamylamine MEC). After 24 hours, area at risk, infarct size, inflammation parameters, apoptosis were...
The extracellular matrix (ECM) from perilesional and colorectal carcinoma (CRC), but not healthy colon, sustains proliferation invasion of tumor cells. We investigated the biochemical physical diversity ECM in pair-wised comparisons healthy, CRC specimens. Progressive linearization degree organization fibrils was observed to ECM, associated with a steady increase stiffness collagen crosslinking. In these modifications coincided increased vascularization, whereas neoplastic they were altered...
In Creutzfeldt-Jakob disease (CJD), molecular typing based on the size of protease resistant core disease-associated prion protein (PrP(Sc) ) and M/V polymorphism at codon 129 PRNP gene correlates with clinico-pathologic subtypes. Approximately 95% sporadic 129MM CJD patients are characterized by cerebral deposition type 1 PrP(Sc) correspond to classic clinical phenotype. The rare 2 further subdivided in a cortical thalamic form also indicated as fatal insomnia. We observed two young...
Abstract The lack of clinically-reliable biomarkers makes impossible to predict sperm retrieval outcomes at testicular extraction (TESE) in men with non-obstructive azoospermia (NOA), resulting up 50% unnecessary surgical interventions. Clinical data, hormonal profile and histological classification testis parenchyma from 47 white-Caucasian idiopathic NOA (iNOA) submitted microdissection TESE (microTESE) were analyzed. Logistic regression analyses tested potential clinical predictors...
The interplay between tumor cells and the microenvironment has been recognized as one of hallmarks cancer biology. To assess role extracellular matrix (ECM) in modulation tissue homeostasis tumorigenesis, we developed a protocol for purification tissue-derived ECM using mucosae from healthy human colon, perilesional area, colorectal carcinoma (CRC). Matched specimens were collected left colon patients undergoing CRC resection surgery. ECMs obtained tissues that decellularized with hypotonic...
Crohn's disease (CD) is a chronic bowel inflammation that ultimately leads to fibrosis, for which medical therapy currently unavailable. Fibrotic strictures in CD are characterized by excessive extracellular matrix (ECM) deposition, altered balance between metalloproteinases (MMPs) and their tissue inhibitors (TIMPs), overexpression of fibroblast activation protein (FAP), marker active fibroblasts. Here we investigated the role FAP-targeted ECM remodeling ex vivo.Bowel specimens were...
Abstract JC virus (JCV) is a polyomavirus that asymptomatically infects up to 80% of the worldwide human population and establishes latency in kidney. In case host immunodeficiency, it can cause progressive multifocal leukoencephalopathy (PML), which fatal demyelinating disease central nervous system. an attempt understand better PML pathogenesis JCV infection, presence genome expression early viral protein brain deceased individuals, with without HIV was investigated. Sixty autopsy samples...
COVID-19 breakout in Italy has caused a huge number of severely ill patients with serious increase mortality. Although lungs seem to be the main target infection very few information are available about liver involvement infection, that could possibly evocate systemic disease targeting lot organs. Since now there no reports large series histological evaluation morphology this setting. Knowledge findings connected clinical data is crucial management disease.Post-mortem wedge biopsies from 48...
Objective: To evaluate the prevalence of HIV-related central nervous system (CNS) lesions (HIV-encephalitis and/or HIV-leukoencephalopathy: HIV-E/L) with and without concomitant opportunistic diseases in a large autopsy series, to correlate it changes antiretroviral treatment that have occurred since beginning epidemic. Methods: We reviewed 1597 consecutive autopsies HIV-positive patients performed between 1984 2000, divided into four time periods on basis therapeutic regimens available:...
Abstract In the fields of biomaterials and tissue engineering simulating native microenvironment is utmost importance. As a major component microenvironment, extracellular matrix (ECM) contributes to homeostasis, whereas modifications features are associated with pathological conditions. Furthermore, three-dimensional (3D) geometry an important feature synthetic scaffolds favoring cell stemness, maintenance differentiation. We analyzed 3D structure, geometrical measurements anisotropy ECM...
To study pathogenic features of the somatic testicular microenvironment associated with idiopathic germ cell aplasia.Cross-sectional study.Tertiary referral center for reproductive medicine.Testicular specimens from men nonobstructive azoospermia (iNOA) prospectively submitted to microdissection sperm extraction. Of 20 used histology, 10 were also available proteomic analysis. Primary Sertoli cells normal karyotype and phenotype used.Patients iNOA dichotomized according a positive versus...
The ongoing SARS-CoV-2 pandemic has proven to be challenging, in both clinical and pathological terms. viral tropism for angiotensin-converting enzyme 2 (ACE-2) receptor-expressing cells is paralleled the setting by development of a spectrum manifestations, most notably acute respiratory distress syndrome (ARDS).1-3 Neurological symptoms are frequent COVID-19 patients; however, central nervous system (CNS) infection subsequent neurological disease attributable remains still poorly defined...
Objectives To identify predictive factors for moderate/severe liver fibrosis and to analyse progression in paired biopsies from HIV‐positive patients with chronic hepatitis C virus (HCV) infection. Methods HIV/HCV coinfected followed at the 2nd Department of Infectious Diseases L. Sacco Hospital Milan, Italy, least one biopsy specimen were retrospectively evaluated. Results A total 110 enrolled study. In a univariate analysis, Ishak–Knodell stage ≥3 history alcohol abuse [odds ratio (OR)...
Glomerular involvement in HIV-positive patients is quite heterogeneous. In the present paper we reviewed 73 renal biopsies performed during a period of more than 20 years single Nephrology Unit, Milan, Northern Italy, order to evaluate aspects types glomerular lesions (including HIV associated nephropathy-HIVAN), grouped according histological patterns and clinical presentation. Moreover, group non-HIVAN patients, possible differences characteristics from non-HIV were investigated.Renal...
Aliment Pharmacol Ther 2011; 34: 1088–1097 Summary Background Ulcerative colitis (UC) is characterised by impaired fatty-acid oxidation; l-carnitine has a key role in metabolism and short-chain fatty acids such as butyrate propionate are important energy source for intestinal epithelial cells. Aim To evaluate efficacy safety of colon-release propionyl-l-carnitine (PLC) patients with mild-to-moderate UC receiving stable oral aminosalicylate or thiopurine therapy. Methods In multicentre, phase...
The aim of this study was the analysis geometrical relationships between different structures constituting aortic root, with particular attention to interleaflet triangles, haemodynamic ventriculo-arterial junction and functional annulus in normal subjects.Sixteen formol-fixed human hearts roots were studied. root isolated, sectioned at midpoint non-coronary sinus, spread apart photographed by a high-resolution digital camera. After calibration picture resizing, software AutoCAD 2004 used...
The role of cytomegalovirus (CMV) reactivation in the outcome inflammatory bowel diseases (IBDs) is unclear. Uncertainties remain on treatment CMV infection, especially patients with a viral load just above normal value blood or very few positive cells colonic biopsies. This retrospective cohort study reports long-term 38 active IBD 13 whom received an antiviral treatment.Thirty ulcerative colitis (UC) and eight Crohn's diagnosed by detection at least one inclusion histology...