A5011206324- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Gold and Silver Nanoparticles Synthesis and Applications
- RNA Interference and Gene Delivery
- Advanced Drug Delivery Systems
- Advanced Biosensing Techniques and Applications
- Nanoparticles: synthesis and applications
- Transplantation: Methods and Outcomes
- Graphene and Nanomaterials Applications
- Characterization and Applications of Magnetic Nanoparticles
- Nanoplatforms for cancer theranostics
- Neonatal Respiratory Health Research
- Inhalation and Respiratory Drug Delivery
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Nanocluster Synthesis and Applications
- HER2/EGFR in Cancer Research
- Cancer Cells and Metastasis
- Click Chemistry and Applications
- Advancements in Transdermal Drug Delivery
- Curcumin's Biomedical Applications
- Occupational and environmental lung diseases
- Systemic Sclerosis and Related Diseases
- Quantum Dots Synthesis And Properties
- Extracellular vesicles in disease
University of Milano-Bicocca
2016-2025
Mylan (Switzerland)
2019-2021
Milano University Press
2019
Luigi Sacco Hospital
2010-2018
University of Milan
2008-2018
Istituti Clinici Scientifici Maugeri
2018
Institut de Biosciences et Biotechnologies
2016
Don Carlo Gnocchi Foundation
2011-2013
Institute of Molecular Science and Technologies
2008-2010
PLGA nanoparticles are among the most studied polymer nanoformulations for several drugs against different kinds of malignant diseases, thanks to their in vivo stability and tumor localization exploiting well-documented "enhanced permeation retention" (EPR) effect. In this paper, we have developed uniform curcumin-bearing by a single-emulsion process, which exhibited curcumin release following Fickian-law diffusion over 10 days vitro. were about 120 nm size, as determined dynamic light...
The discovery of new solutions with antibacterial activity as efficient and safe alternatives to common preservatives (such parabens) combat emerging infections drug-resistant bacterial pathogens is highly expected in cosmetics pharmaceutics. Colloidal silver nanoparticles (NPs) are attracting interest novel effective antimicrobial agents for the prevention several infectious diseases.Water-soluble, negatively charged (AgNPs) were synthesized by reduction citric tannic acid characterized...
Abstract Active targeting of nanoparticles to tumours can be achieved by conjugation with specific antibodies. Specific active the HER2 receptor is demonstrated in vitro and vivo a subcutaneous MCF-7 breast cancer mouse model trastuzumab-functionalized gold nanoparticles. The number attached antibodies per nanoparticle was precisely controlled way that each conjugated either exactly one or two As expected, we found moderate increase efficiency instead just antibody nanoparticle. However,...
Triple negative breast cancer (TNBC) is a highly aggressive, invasive, and metastatic tumor. Although it reported to be sensitive cytotoxic chemotherapeutics, frequent relapse chemoresistance often result in treatment failure. In this study, we developed biomimetic nanodrug consisting of self-assembling variant (HFn) human apoferritin loaded with curcumin. HFn nanocage improved the solubility, chemical stability, bioavailability curcumin, allowing us reliably carry out several experiments...
A great challenge in nanodiagnostics is the identification of new strategies aimed to optimize detection primary breast cancer and metastases by employment target-specific nanodevices. At present, controversial proof has been provided on actual importance surface functionalization nanoparticles improve their vivo localization at tumor. In present paper, we have designed developed a set multifunctional nanoprobes, modified with three different variants model antibody, that is, humanized...
Targeted therapies have profoundly changed the clinical prospect in human epidermal growth factor receptor 2 (HER2)-positive breast cancer. In particular, anti-HER2 monoclonal antibody trastuzumab represents gold standard for treatment of HER2+ cancer patients. Its contribution dampening progression is mainly attributed to antibody-dependent cell-mediated cytotoxicity (ADCC) rather than HER2 blockade. Here, multiple half chains were conjugated onto magnetic iron oxide nanoparticles (MNP-HC)...
We report the development of an efficient antibody delivery system for incorporation trastuzumab (TZ) into poly(lactic-co-glycolic) acid nanoparticles (PLGA NPs). The aim work was to overcome current limitations in clinical use therapeutic antibodies, including immunogenicity, poor pharmacokinetics, low tumor penetration and safety issues.Trastuzumab-loaded PLGA NPs (PLGA-TZ) were synthesized according a double emulsion method. same protocol used produce control batches nonspecific...
The authentication process involves all the supply chain stakeholders, and it is also adopted to verify food quality safety. Food tools are an essential part of traceability systems as they provide information on credibility origin, species/variety identity, geographical provenance, production entity. Moreover, these useful evaluate effect transformation processes, conservation strategies reliability packaging distribution flows In this manuscript, we identified innovative characteristics...
Highly monodisperse magnetite nanocrystals (MNC) were synthesized in organic media and transferred to the water phase by ultrasound-assisted ligand exchange with an iminodiacetic phosphonate. The resulting biocompatible magnetic nanoparticles characterized transmission electron microscopy, dynamic light scattering, magnetorelaxometry, indicating that this method allowed us obtain stable particle dispersions narrow size distribution unusually high resonance T2 contrast power. These conjugated...
Particularly suitable: An N-terminal serine mutant of anti-HER2 scFv antibody was conjugated to polymer-coated magnetofluorescent nanoparticles by strain-promoted alkyne–nitrone cycloaddition. The resulting (see scheme) proved effective in targeting and labeling HER2-positive breast cancer cells.
Subcellular destiny of targeted nanoparticles in cancer cells within living organisms is still an open matter debate. By vivo and ex experiments on tumor-bearing mice treated with antibody-engineered magnetofluorescent nanocrystals, which we combined fluorescence imaging, magnetic relaxation, trasmission electron microscopy approaches, provide evidence that are effectively delivered to the tumor by active targeting. These nanocrystals were demonstrated enable contrast enhancement resonance...
Aims: Chronic lung allograft dysfunction represents the main cause of death after transplantation, and so far there is no effective therapy. Mesenchymal cells (MCs) are primarily responsible for fibrous obliteration small airways typical chronic dysfunction. Here, we engineered gold nanoparticles containing a drug in hydrophobic section to inhibit MCs, exposing on outer hydrophilic surface monoclonal antibody targeting MC-specific marker (half-chain with everolimus). Materials & methods:...
Abstract The identification of a highly sensitive method to check the delivery administered nanodrugs into tumor cells is crucial step preclinical studies aimed develop new nanoformulated cures, since it allows real therapeutic potential these devices be forecast. In present work, ability an H‐ferritin (HFn) nanocage, already investigated as powerful tool for cancer therapy thanks its actively interact with transferrin receptor 1, act efficient probe monitoring nanodrug tumors demonstrated....