A5061448584- Wheat and Barley Genetics and Pathology
- Monoclonal and Polyclonal Antibodies Research
- Transgenic Plants and Applications
- Plant Micronutrient Interactions and Effects
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Protein purification and stability
- Glycosylation and Glycoproteins Research
- Biofuel production and bioconversion
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Genetics and Plant Breeding
- Bacteriophages and microbial interactions
- Rice Cultivation and Yield Improvement
- Crop Yield and Soil Fertility
- Advanced Biosensing Techniques and Applications
- Toxin Mechanisms and Immunotoxins
- Agricultural Productivity and Crop Improvement
- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- Soybean genetics and cultivation
- Phytoplasmas and Hemiptera pathogens
- Cynara cardunculus studies
- Plant responses to elevated CO2
- Hemophilia Treatment and Research
National Agency for New Technologies, Energy and Sustainable Economic Development
2011-2024
ENEA Casaccia Research Centre
2008-2024
National Center for Genetic Engineering and Biotechnology
2004-2008
University of Oxford
1987
Sapienza University of Rome
1986
Human urokinase cDNA clones have been identified from a library prepared total RNA of human fibroblasts transformed by simian virus 40 [Okayama, H. & Berg, P. (1983) Mol. Cell. Biol. 3, 280-289]. Synthetic oligonucleotides, corresponding to protein sequence, were used as probes. The cloned covers most the coding sequence and entire 3' untranslated region. nucleotide one identifies this copy partially spliced polyadenylylated precursor mRNA. introns separate functionally different domains...
Particularly suitable: An N-terminal serine mutant of anti-HER2 scFv antibody was conjugated to polymer-coated magnetofluorescent nanoparticles by strain-promoted alkyne–nitrone cycloaddition. The resulting (see scheme) proved effective in targeting and labeling HER2-positive breast cancer cells.
The promotion of renewable energy represents a target the European 2020 strategy for growth. Plant biomass and organic wastes from agriculture represent an effective resource to be exploited sustainable rural development, optimizing land use, diversifying entrepreneurship. Cereals are considered promising producing crop in temperate regions Europe used both fuel alcohol biogas production. In particular, triticale shows number advantages such as high grain yield even marginal environments,...
Aberrant signaling by ErbB-2 (HER 2, Neu), a member of the human Epidermal Growth Factor (EGF) receptor family, is associated with an aggressive clinical behaviour carcinomas, particularly breast tumors. Antibodies targeting pathway are preferred therapeutic option for patients advanced cancer, but worldwide deficit in manufacturing capacities mammalian cell bioreactors foreseen.Herein, we describe multi-platform approach production recombinant Single chain Fragments antibody variable...
Abstract Genes from the DREB family are involved in plant’s responses to dehydration and possibly play a role their ability tolerate water stress. Understanding relationship between stress tolerance expression of specific genes requires isolation characterisation sequences that may be involved. We report gene Triticum durum , namely TdDRF1 which belongs produces three forms transcripts through alternative splicing. The profile was assessed by real‐time reverse transcription–polymerase chain...
Phenylalanine Ammonia Lyase (PAL) gene which plays a key role in bio-synthesis of medicinally important compounds, Rutin/quercetin was sequence characterized for its efficient genomics application. These compounds possessing anti-diabetic and anti-cancer properties are predominantly produced by Fagopyrum spp. In the present study, PAL sequenced from three (F. tataricum, F. esculentum dibotrys) showed presence SNPs four insertion/deletions at intra inter specific level. Among them, potential...
Homologous “propeptide” regiona are present in a family of vitamin κ-dependent mammalian proteins, including clotting factors II, VII, IX, X, protein C, S and bone “gla” proteins. To test the hypothesis that propeptide is signal for correct γ-carboxylation adjacent γ-carboxy region, we have mutated amino acid -4 human factor IX from an arginim to glutamine residue, by M13-directed site-specific mutagenesis cDNA clone. After expression mutant dog kidney cells, find it secreted into medium...
The surface activation of multifunctional nanoparticles (MNPs) with peptide ligands directing their targeting to cancer cells is an emerging research area in nanobiotechnology. In this paper, water-soluble MNPs have been synthesized and functionalized scFv antibody variant specific toward the HER2 receptor overexpressed several breast cell lines. was genetically engineered introduce a cysteine residue inside loop sequence bridging VH VL lobes molecule histidine tag at C-terminus fragment....
ABSTRACT Recombinant single-chain variable fragment antibodies (scFv) that bind specifically to Citrus tristeza virus (CTV), which cause the most detrimental viral disease in citrus industry worldwide, were obtained from hybridoma cell lines 3DF1 and 3CA5. These scFv genetically fused with dimerization domains as well alkaline phosphatase, respectively, diagnostic reagents produced by expressing these fusion proteins bacterial cultures. The engineered successfully used for CTV diagnosis...
Tomato plants (Lycopersicon esculentum Mill.) showing leaf yellowing or reddening, proliferation of lateral shoots, hypertrophied calyces, and greening petals were found in four tomato-growing areas central Italy during surveys carried out 1999–2000. The highest disease incidence was recorded 1999 (78%) 2000 (82%) near Ronciglione (Viterbo province). phytoplasmas associated with the detected characterised using PCR RFLP. They belonged to 16S rRNA groups I, III, V XII (sensu Lee et al., 1998....
Journal Article cDNA sequence coding for human coagulation factor XII (Hageman) Get access M. Tripodi, Tripodi *European Molecular Biology LaboratoryMeyerthofstrsse 1, 6900 Heidelberg, FRG Search other works by this author on: Oxford Academic PubMed Google Scholar F. Citraella, Citraella S. Guida, Guida P. Galeffi, Galeffi A. Fantoni, Fantoni R. Cortese Nucleic Acids Research, Volume 14, Issue 7, 11 April 1986, Page 3146, https://doi.org/10.1093/nar/14.7.3146 Published: 1986 history...
Eine N-terminale Serinmutante des Anti-HER2-Antikörpers scFv wurde durch die Titelreaktion an polymerbeschichtete magnetofluoreszierende Nanopartikel konjugiert (siehe Schema), sich anschließend als sehr effektiv für Erkennung und Markierung HER2-positiver Brustkrebszellen erwiesen.
Spherical silica nanoparticles (SNP) have been synthesized and functionalized with anti-HER-2 scFv800E6 antibody by both localized histidine-tag recognition, leading to an oriented protein ligation, glutaraldehyde cross-linking, exploiting a statistical reactivity of lysine amine groups in the primary sequence molecule. The targeting efficiency nanocomplexes comparison free scFv was evaluated flow cytometry using HER-2 antigen-positive MCF-7 breast cancer cell line, exhibiting 4-fold...
Abstract Monoclonal antibodies coupled to highly toxic molecules (immunoconjugates) are currently being developed for cancer therapy. We have used an in silico procedure evaluating some physicochemical properties of two tumor-targeting anti-HER2 immunoconjugates: (a) the single-chain antibody scFv(FRP5) linked a bacterial toxin, that has been recently progressed phase I clinical trial human cancer; (b) putative molecule formed by intrinsically stable scFv(800E6), which proposed as toxin...
This study aims to highlight the major effects of biochar incorporation into potting soil substrate on plant growth and performance in early stages five elite Italian varieties durum wheat ( Triticum ). The biochars used were obtained from two contrasting feedstocks, namely wood chips straw, by gasification under high temperature conditions, applied a greenhouse experiment either as pure or nutrient-activated incubation with digestate. results showed that specific genotypes well different...
Abstract The genus Aegilops L. has been intensively investigated due to its close relationship with wheat ( Triticum L.) as contributor of B and D subgenomes. Because their vast genetic diversity, species represent a rich source alleles agronomic interest, which could be used widen the gene pool improve tolerance diseases, pests, drought, cold other environmental stresses. We report isolation characterization Dehydration Responsive Factor 1 DRF1) in three accessions Ae. speltoides coming...