A5088593644- Polyamine Metabolism and Applications
- Plant Stress Responses and Tolerance
- Amino Acid Enzymes and Metabolism
- Plant tissue culture and regeneration
- Microbial metabolism and enzyme function
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Cassava research and cyanide
- Transgenic Plants and Applications
- Enzyme-mediated dye degradation
- Plant Gene Expression Analysis
- Plant responses to water stress
- Pesticide and Herbicide Environmental Studies
- GABA and Rice Research
- Porphyrin Metabolism and Disorders
- Toxin Mechanisms and Immunotoxins
- Legume Nitrogen Fixing Symbiosis
- Cannabis and Cannabinoid Research
- Plant Reproductive Biology
- Epigenetics and DNA Methylation
- Light effects on plants
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Soybean genetics and cultivation
- Microbial bioremediation and biosurfactants
Istituto Nazionale Biostrutture e Biosistemi
2015-2024
Roma Tre University
2014-2024
Marconi University
2000-2017
Instituto de Biología Molecular y Celular de Plantas
2006
Consejo Superior de Investigaciones Científicas
2006
Universitat Politècnica de València
2006
University of Leicester
2005
University of Bari Aldo Moro
2004
University of Bologna
2004
Tecnologia Energia Ambiente Materiali (Italy)
2004
Abstract Polyamine oxidase (PAO) is a flavin adenine dinucleotide-dependent enzyme involved in polyamine catabolism. Animal PAOs oxidize spermine (Spm), spermidine (Spd), and/or their acetyl derivatives to produce H2O2, an aminoaldehyde, and Spd or putrescine, respectively, thus being back-conversion pathway. On the contrary, plant that have been characterized date Spm 1,3-diaminopropane, aminoaldehyde are therefore terminal catabolism of polyamines. A database search within Arabidopsis...
Polyamine oxidases (PAOs) are FAD-dependent enzymes involved in polyamine catabolism. All so far characterized PAOs from monocotyledonous plants, such as the apoplastic maize PAO, oxidize spermine (Spm) and spermidine (Spd) to produce 1,3-diaminopropane, H2O2, an aminoaldehyde, thus considered be a terminal catabolic pathway. Mammalian Spm or Spd (and/or their acetyl derivatives) differently PAOs, producing putrescine, respectively, addition H2O2 therefore back-conversion In Arabidopsis...
Polyamine oxidases (PAOs) are flavin-dependent enzymes involved in polyamine catabolism. In Arabidopsis five PAO genes (AtPAO1-AtPAO5) have been identified which present some common characteristics, but also important differences primary structure, substrate specificity, subcellular localization, and tissue-specific expression pattern, may suggest distinct physiological roles. the work, AtPAO5, only so far uncharacterized AtPAO is specifically expressed vascular system, was partially...
Abstract To test the feasibility of altering polyamine levels by influencing their catabolic pathway, we obtained transgenic tobacco (Nicotiana tabacum) plants constitutively expressing either maize (Zea mays) oxidase (MPAO) or pea (Pisum sativum) copper amine (PCuAO), two extracellular and H2O2-producing enzymes. Despite high expression transgenes in space, amount free polyamines homozygous was similar to that wild-type ones, suggesting a tight regulation different compartmentalization...
The first complete amino acid sequence of a flavin-containing polyamine oxidase was solved by combined approach nucleotide and peptide analysis. A cDNA 1737 bp, isolated from maize seedlings reverse transcription-polymerase chain reaction rapid amplification ends strategies, cloned its determined. This contains information for polypeptide 500 acids. Its amino-terminal shows the typical features secretion signal peptides. primary structure mature protein independently confirmed extensive...
Polyamines are involved in several plant physiological processes. In Arabidopsis thaliana, five FAD-dependent polyamine oxidases (AtPAO1 to AtPAO5) contribute homeostasis. AtPAO5 catalyzes the back-conversion of thermospermine (T-Spm) spermidine and plays a role development, xylem differentiation, abiotic stress tolerance. present study, verify whether T-Spm metabolism can be exploited as new route improve tolerance crops investigate underlying mechanisms, tomato (Solanum lycopersicum)...
The mRNA coding for light-harvesting complex of PSII (LHC-II) apoprotein is present in etiolated bean (Phaseolus vulgaris L.) leaves; its level low 5-day-old leaves, increases about 3 to 4 times 9- 13-day-old and decreases thereafter. A red light pulse induces an increase LHC-II level, which reversed by far light, all ages the tissue tested. phytochrome-controlled initial higher than 5- 17-day-old leaves. amount mRNA, accumulated dark after a pulse, oscillates rhythmically with period 24...
In plants, the polyamines putrescine, spermidine, spermine (Spm), and thermospermine (Therm-Spm) participate in several physiological processes. particular, Therm-Spm is involved control of xylem differentiation, having an auxin antagonizing effect. Polyamine oxidases (PAOs) are FAD-dependent enzymes polyamine catabolism. Arabidopsis, five PAOs present, among which AtPAO5 catalyzes back-conversion Spm, Therm-Spm, N1-acetyl-Spm to spermidine. present study, it shown that two loss-of-function...
Polyamines are involved in key developmental processes and stress responses. Copper amine oxidases oxidize the polyamine putrescine (Put), producing an aldehyde, ammonia, hydrogen peroxide (H2O2). The Arabidopsis (Arabidopsis thaliana) oxidase gene At4g14940 (AtAO1) encodes apoplastic copper expressed at early stages of vascular tissue differentiation roots. Here, its role root development xylem was explored by pharmacological forward/reverse genetic approaches. Analysis AtAO1 expression...
Abstract The ornithine–urea cycle (urea cycle) makes a significant contribution to the metabolic responses of lower photosynthetic eukaryotes episodes high nitrogen availability. In this study, we compared role plant urea and its relationships polyamine metabolism in ammonium-fed nitrate-fed Medicago truncatula plants. High ammonium resulted accumulation pathway intermediates, particularly glutamine, arginine, ornithine, putrescine. Arginine decarboxylase activity was decreased roots,...
In Arabidopsis thaliana, four FAD-dependent lysine-specific histone demethylases (LDL1, LDL2, LDL3, and FLD) are present, bearing both a SWIRM an amine oxidase domain. this study, comparative analysis of gene structure, evolutionary relationships, tissue- organ-specific expression patterns, physiological roles target genes for the LDL/FLDs is reported. Phylogenetic evidences different history LDL/FLDs, while promoter activity data show that strongly expressed during plant development...
Abstract Polyamine oxidases (PAOs) are characterized by a broad variability in catalytic properties and subcellular localization, impact key cellular processes diverse organisms. In the present study, comprehensive phylogenetic analysis was performed to understand evolution of PAOs across three domains life particularly within eukaryotes. Phylogenetic trees show that PAO-like sequences bacteria, archaea, eukaryotes form distinct clades, with exception few procaryotes probably acquired PAO...
Maize polyamine oxidase (MPAO), the only member of (PAO) family whose three-dimensional structure is known, characterized by a 30 A long U-shaped catalytic tunnel located between substrate binding domain and FAD. To shed light on MPAO ligand mode, we studied inhibition properties linear diamines, agmatine, prenylagmatine (G3), G3 analogues, guazatine, analyzed structural determinants their biological activity. Linear diamines competitively inhibited MPAO, with inhibitory activity increasing...
Maize polyamine oxidase (MPAO) is a flavin adenine dinucleotide (FAD)-dependent enzyme that catalyses the oxidation of spermine and spermidine at secondary amino groups. The structure MPAO indicates 30-A long U-shaped tunnel forms catalytic site, with residues Glu62 Glu170 located close to enzyme-bound FAD residue Tyr298 in proximity Lys300, which turn hydrogen-bonded N(5) atom via water molecule (HOH309). To provide insight into role these mechanism reduction, we have performed steady-state...
Arabidopsis thaliana has four genes with close homology to human histone H3 lysine 4 demethylase (HsLSD1), a component of various transcriptional corepressor complexes that often also contain deacetylases and the protein CoREST. All proteins flavin amine oxidase domain SWIRM domain, latter being present in number involved chromatin regulation. Here, we describe heterologous expression biochemical characterization one these (AtLSD1) show that, similarly HsLSD1, it activity toward mono-...