A5082347305- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Essential Oils and Antimicrobial Activity
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Edible Oils Quality and Analysis
- Adipose Tissue and Metabolism
- RNA and protein synthesis mechanisms
- Medicinal Plants and Neuroprotection
- Listeria monocytogenes in Food Safety
- Alzheimer's disease research and treatments
- Polysaccharides and Plant Cell Walls
- Amino Acid Enzymes and Metabolism
- Polyamine Metabolism and Applications
- Salmonella and Campylobacter epidemiology
- Food Safety and Hygiene
- Reproductive tract infections research
- Cassava research and cyanide
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Fluid Dynamics and Heat Transfer
- Sulfur Compounds in Biology
- Plant and animal studies
Sapienza University of Rome
2009-2024
Roma Tre University
2020-2024
Azienda USL di Bologna
2001-2012
Politecnico di Milano
2008
University of Milan
2003
University of Florence
1979
Overexpression of the proline biosynthetic gene P5CS1 results in early flowering Arabidopsis. However, p5cs1 loss-of-function mutant exhibits a modest delay flowering, suggesting that P5CS2, duplicated present Arabidopsis, may also play role flower transition. In situ mRNA hybridizations and quantitative reverse transcription-polymerase chain reaction (RT-PCR) revealed P5CS2 are expressed at similar levels with same pattern expression vegetative floral shoot apical meristems as well axillary...
In crosses between the proline-deficient mutant homozygous for p5cs1 and heterozygous p5cs2 (p5cs1 p5cs2/P5CS2), used as male, different Arabidopsis mutants, females, allele was rarely transmitted to outcrossed progeny, suggesting that fertility of male gametophyte carrying mutations in both P5CS1 P5CS2 is severely compromised.To confirm defects pollen from p5cs2/P5CS2 transmission alleles through tested two ways. First, number progeny inheriting a dominant sulfadiazine resistance marker...
We reported previously that root elongation in Arabidopsis is promoted by exogenous proline, raising the possibility this amino acid may modulate growth.To evaluate hypothesis we used a combination of genetic, pharmacological and molecular analyses, showed proline specifically affects growth modulating size meristem. The effects on meristem are parallel to, independent from, hormonal pathways, do not involve expression genes controlling cell differentiation at transition zone. On contrary,...
Alzheimer's disease (AD) is the most common neurodegenerative disorder and primary form of dementia in elderly. One main features AD increase amyloid-beta (Aβ) peptide production aggregation, leading to oxidative stress, neuroinflammation neurodegeneration. Polyphenols are well known for their antioxidant, anti-inflammatory neuroprotective effects have been proposed as possible therapeutic agents against AD. Here, we investigated a polyphenolic extract Arabidopsis thaliana (a plant belonging...
We previously reported that proline modulates root meristem size in Arabidopsis by controlling the ratio between cell division and differentiation. Here, we show metabolism affects levels of superoxide anion (O2•-) hydrogen peroxide (H2O2), which, turn, modulate elongation. found plays a major role proline-mediated elongation, its effects largely overlap those induced proline, influencing size, cycle. Though combination genetic pharmacological evidence, showed short-root phenotype...
Polyamines are involved in several plant physiological processes. In Arabidopsis thaliana, five FAD-dependent polyamine oxidases (AtPAO1 to AtPAO5) contribute homeostasis. AtPAO5 catalyzes the back-conversion of thermospermine (T-Spm) spermidine and plays a role development, xylem differentiation, abiotic stress tolerance. present study, verify whether T-Spm metabolism can be exploited as new route improve tolerance crops investigate underlying mechanisms, tomato (Solanum lycopersicum)...
The genetic basis of stem cell specification in somatic embryogenesis and organogenesis is still obscure. SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) genes are involved numerous species. In vitro culture Cyclamen persicum immature ovules provides a system for investigating formation maintenance, because lines forming either organs or embryos callus without organs/embryos available the same cultivar plant growth regulator conditions. present aim was to exploit this property cyclamen...
β -Amyloid peptide (A ) aberrant production and aggregation are major factors implicated in the pathogenesis of Alzheimer’s disease (AD), causing neuronal death via oxidative stress. Several studies have highlighted importance polyphenolic antioxidant compounds treatment AD, but complex food matrices, characterized by a different relative content these phytochemicals, been neglected. In present study, we analyzed protective effect on SH-SY5Y cells treated with fragment A 25–35 two crude...
In many plants, the amino acid proline is strongly accumulated in pollen and disruption of synthesis caused abortion microspore development Arabidopsis. So far, it was unclear whether local biosynthesis or transport determines success fertile development. We analyzed expression pattern biosynthetic genes PYRROLINE-5-CARBOXYLATE SYNTHETASE 1 & 2 (P5CS1 2) Arabidopsis anthers both isoforms were expressed developing microspores grains but only inconsistently surrounding sporophytic tissues....
Seed yield, a major determinant for the commercial success of grain crops, critically depends on pollen viability, which is dramatically reduced by environmental stresses, such as drought, salinity, and extreme temperatures. Salinity, in particular, problem crop yield known to affect about 20% all arable land cause huge economic losses worldwide. Flowering plants are particularly sensitive stress during sexual reproduction, even short exposure stressing conditions can severely hamper...
The endocannabinoid system is involved in the nociceptive and anti-inflammatory pathways, a lowered expression of CB2 receptors has been associated with inflammatory conditions, such as osteoarthritis (OA). This suggests that modulators could be novel therapeutic tools to treat OA. In present study, involvement Harpagophytum procumbens root extract, common ingredient nutraceuticals used joint disorders, modulation evaluated. Moreover, clarify effects pure single components, bioactive...
The direct sale by farmers of raw milk for human consumption has been allowed in Italy since 2004. aim this study was to evaluate the behaviour selected foodborne pathogens sold vending machines, field handling conditions, and during shelf-life from production consumption. Temperature storage 33 farms authorized produce sell were investigated farm machine delivery, together with consumer habits one province Emilia-Romagna region northern Italy. Failure maintain appropriate low temperatures...
Objectives: Renal damage precedes occurrence of stroke in high-sodium/low-potassium-fed stroke-prone spontaneously hypertensive rat (SHRSP). We previously reported a marked suppression uncoupling protein-2 (UCP2) upon high-salt Japanese-style diet SHRSP kidneys. Vegetable compounds are known to exert protective effects cardiovascular diseases. aimed at evaluating the impact Brassica oleracea sprouts juice toward renal Japanese diet-fed and exploring role 5′-adenosine monophosphate-activated...
New antimicrobial molecules effective against Pseudomonas aeruginosa, known as an antibiotic-resistant "high-priority pathogen", are urgently required because of its ability to develop biofilms related healthcare-acquired infections. In this study, for the first time, anti-biofilm and anti-virulence activities a polyphenolic extract extra-virgin olive oil well purified oleocanthal oleacein, toward P. aeruginosa clinical isolates were investigated. The main result our study was activity...
The recent finding that proline-induced root elongation is mediated by reactive oxygen species (ROS) prompted us to re-evaluate other developmental processes modulated proline, such as flowering time. By controlling the cellular redox status and ROS distribution, proline could potentially affect expression of transcriptional factors subjected epigenetic regulation, FLOWERING LOCUS C (FLC). Accordingly, we investigated effect on time in more detail analyzing relative main genes p5cs1...