A5019161572- Heart Failure Treatment and Management
- Adipose Tissue and Metabolism
- Renin-Angiotensin System Studies
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Cardiovascular Function and Risk Factors
- Fatty Acid Research and Health
- Neurological Disease Mechanisms and Treatments
- ATP Synthase and ATPases Research
- Nuclear Receptors and Signaling
- Eicosanoids and Hypertension Pharmacology
- Diet and metabolism studies
- GDF15 and Related Biomarkers
- Biochemical effects in animals
- Kruppel-like factors research
- Acute Myocardial Infarction Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cardiac Fibrosis and Remodeling
- Atrial Fibrillation Management and Outcomes
- Cardiac Valve Diseases and Treatments
- MicroRNA in disease regulation
- Birth, Development, and Health
- Potassium and Related Disorders
- Blood Coagulation and Thrombosis Mechanisms
- PI3K/AKT/mTOR signaling in cancer
Istituto Neurologico Mediterraneo
2015-2024
Sapienza University of Rome
2003-2015
Centro Cardiologico Monzino
2013
University of Milan
2013
Azienda Ospedaliera Sant'Andrea
2005-2013
Rutgers, The State University of New Jersey
2013
Rutgers New Jersey Medical School
2013
Onze Lieve Vrouwziekenhuis Hospital
2013
William Harvey Research Institute
2013
Vita-Salute San Raffaele University
2005
The identification of the mechanisms predisposing to stroke may improve its preventive and therapeutic strategies in patients with essential hypertension. role macroautophagy/autophagy development hypertension-related needs be clarified. We hypothesized that a defective autophagy favor spontaneous by promoting mitochondrial dysfunction. studied stroke-prone spontaneously hypertensive (SHRSP) rat, which represents clinically relevant model associated high blood pressure. assessed autophagy,...
Background The genetic basis of stroke susceptibility remains to be elucidated. STR 1 quantitative trait locus ( 1/ QTL ) was identified on rat chromosome stroke‐prone spontaneously hypertensive SHRSP upon Japanese‐style stroke‐permissive diet JD ), and it contributes 20% the phenotype variance. Methods Results Nine hundred eighty‐six probe sets mapping were selected from Rat RAE 230A array screened through a microarray differential expression analysis in brains stroke‐resistant SHR SHRSR...
Background and Purpose— A precise definition of genetic factors responsible for common forms stroke is still lacking. The purpose the present study was to investigate contributory role genes encoding atrial natriuretic peptide ( ANP ) type receptor NPRA in humans’ susceptibility develop ischemic stroke. Methods— Allele genotype frequencies were characterized an Italian case-control with patients affected by vascular disease or risk factors. Subjects recruited from island Sardinia (206 cases,...
The stroke-prone spontaneously hypertensive rat (SHRsp) represents an animal model of increased susceptibility to high-salt diet-induced cerebral and renal vascular injuries. High blood pressure genetic factors are viewed as major contributing factors. In high-salt-loaded SHRsp stroke-resistant SHR animals, we determined levels, degree kidney lesions, uncoupling protein 2 (UCP2) gene expression levels along with rattus norvegicus (rno)-microRNA (miR) 24 34a expression, nuclear factor-κB...
Abstract The mitochondrial uncoupling protein 2 (UCP2) plays a protective function in the vascular disease of both animal models and humans. UCP2 downregulation upon high-salt feeding favors dysfunction knock-out mice, accelerates cerebrovascular renal damage stroke-prone spontaneously hypertensive rat. Overexpression counteracts negative effects models. We tested vitro ability to stimulate autophagy mitophagy as mechanism mediating its exposure endothelial tubular cells. silencing reduced...
NPPA/atrial natriuretic peptide (natriuretic type A) exerts critical pleiotropic effects in the cardiovascular system, limiting cardiomyocyte hypertrophy and death, reducing cardiac fibrosis promoting vascular integrity. However, molecular mechanisms underlying these beneficial still need to be clarified. We demonstrated for first time that macroautophagy/autophagy is involved local protective of NPPA cardiomyocytes (CMs), both vitro vivo. Exogenous rapidly activates autophagy CMs through...
Mitochondrial dysfunction, causing increased reactive oxygen species (ROS) production, is a molecular feature of heart failure (HF). A defective antioxidant response and mitophagic flux were reported in circulating leucocytes patients with chronic HF reduced ejection fraction (HFrEF). Atrial natriuretic peptide (ANP) exerts many cardiac beneficial effects, including the ability to protect cardiomyocytes by promoting autophagy. We tested impact ANP on autophagy/mitophagy, altered...
Stroke represents a main cause of death and permanent disability worldwide. The molecular mechanisms underlying cerebral injury in response to the ischemic insults are not completely understood. In this article, we summarize recent evidence regarding role autophagy pathogenesis stroke by reviewing data obtained murine models either transient or middle artery occlusion, stroke-prone spontaneously hypertensive rat. Few preliminary observational studies investigating subjects at high...
Abstract UCP2 maps nearby the lod score peak of STR1 -stroke QTL in SHRSP rat strain. We explored potential contribution to high-salt diet (JD)-dependent increased stroke susceptibility SHRSP. Male SHRSP, SHRSR, two reciprocal SHRSR/SHRSP- STR1/ congenic lines received JD for 4 weeks detect brain gene/protein modulation as compared with regular (RD). Brains were also analyzed NF- κ B protein expression, oxidative stress level and -targeted microRNAs expression level. Next, based on knowledge...
Heart failure is a major side effect of doxorubicin (DOX) treatment in patients with cancer. However, the mechanisms underlying development DOX-induced heart need to be addressed. This study aims test whether serine/threonine kinase MST1, Hippo pathway component, contributes myocardial injury. C57BL/6J WT mice and cardiomyocyte-specific dominant-negative MST1 (kinase-dead) overexpression received three weekly injections DOX, reaching final cumulative dose 18 mg/kg. Echocardiographic,...
The role of the renin-angiotensin-aldosterone system (RAAS) genes on predisposition to develop stroke, a multifactorial and polygenic cardiovascular trait, is still under investigation. In present study we characterized contributory RAAS in susceptibility ischemic stroke humans.Allele genotype frequencies were population 215 cases (including only atherothrombotic lacunar forms) 236 controls selected Sardinia, large Mediterranean island with well-known segregated population. Statistical...
The role of genetic factors in the individual predisposition to develop ischemic stroke has been assessed by previous studies performed both animal models and humans. main goal current investigation was determine possible contribution genes encoding procoagulant inflammatory on occurrence a cohort young cases corresponding controls. One hundred fifteen were recruited for this study. A detailed clinical assessment, definite etiologic diagnosis, as well presence/absence known risk obtained...
C2238 atrial natriuretic peptide (ANP) minor allele (substitution of thymidine with cytosine in position 2238) associates increased risk cardiovascular events.We investigated the mechanisms underlying vascular effects C2238-αANP.In vitro, human umbilical vein endothelial cell were exposed to either wild-type (T2238)- or mutant (C2238)-αANP. Cell survival and apoptosis tested by Trypan blue, annexin V, cleaved caspase-3 assays. C2238-αANP significantly reduced apoptosis. In addition, tube...
Objectives: Renal damage precedes occurrence of stroke in high-sodium/low-potassium-fed stroke-prone spontaneously hypertensive rat (SHRSP). We previously reported a marked suppression uncoupling protein-2 (UCP2) upon high-salt Japanese-style diet SHRSP kidneys. Vegetable compounds are known to exert protective effects cardiovascular diseases. aimed at evaluating the impact Brassica oleracea sprouts juice toward renal Japanese diet-fed and exploring role 5′-adenosine monophosphate-activated...
// Speranza Rubattu 1, 2 , Franca Bianchi Carla Letizia Busceti Maria Cotugno Rosita Stanzione Simona Marchitti Sara Di Castro Michele Madonna Ferdinando Nicoletti 2, 3 Massimo Volpe 1 Department of Clinical and Molecular Medicine, School Medicine Psychology, Sapienza University Rome, Ospedale S. Andrea, Rome Istituto di Ricovero e Cura a Carattere Scientifico (I.R.C.C.S.) Neuromed, Località Camerelle, Pozzilli, Italy Physiology Pharmacology, Correspondence to: Rubattu, e-mail:...
The combination of AT1 blocker/neutroendopeptidase neprilysin inhibition (ARNi) represents an interesting approach to reduce cardiovascular risk in hypertension. We assessed the efficacy ARNi, compared with angiotensin II type 1 receptor blockade alone, on blood pressure (BP) and protection from target organ damage development stroke-prone spontaneously hypertensive rat (SHRSP).In high-salt fed SHRSP, we plasma tissue natriuretic peptides, urinary volume, BP body weight over a short-term...
The compromised viability and function of cardiovascular cells are rescued by small molecules triazole derivatives (Tzs), identified as 3a 3b, preventing mitochondrial dysfunction. oxidative phosphorylation improves the respiratory control rate in presence Tzs independently substrates that energize mitochondria. F1FO-ATPase, main candidate permeability transition pore (mPTP) formation, is biological target hydrophilic F1 domain enzyme depicted binding region Tzs. protective effect Tz on...