A5035815879- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Aquaculture Nutrition and Growth
- Marine Biology and Environmental Chemistry
- Environmental Toxicology and Ecotoxicology
- Marine Bivalve and Aquaculture Studies
- Physiological and biochemical adaptations
- Fuel Cells and Related Materials
- Ion Transport and Channel Regulation
- Meat and Animal Product Quality
- Adipose Tissue and Metabolism
- Metabolism and Genetic Disorders
- Animal Nutrition and Physiology
- Fish Ecology and Management Studies
- Aquatic Invertebrate Ecology and Behavior
- Cardiac Ischemia and Reperfusion
- Ocean Acidification Effects and Responses
- Advanced battery technologies research
- Rabbits: Nutrition, Reproduction, Health
- Botanical Research and Applications
- Fish Biology and Ecology Studies
- Adenosine and Purinergic Signaling
- Analytical Chemistry and Sensors
- Biochemical and Molecular Research
- Diet and metabolism studies
University of Bologna
2016-2025
Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione
2016
Abstract The properties of the mitochondrial F 1 O ‐ATPase catalytic site, which can bind Mg 2+ , Mn or Ca and hydrolyze ATP, were explored by inhibition kinetic analyses to cast light on ‐activated connection with permeability transition pore (PTP) that initiates cascade events leading cell death. While natural cofactor activates in competition is a noncompetitive inhibitor presence . Selective inhibitors (Is‐F ), namely NBD‐Cl, piceatannol, resveratrol, quercetin, exerted different...
Cellular metabolism is an important feature of spermatozoa that deserves more insights to be fully understood, in particular porcine semen physiology. The present study aims characterize the balance between glycolytic and oxidative boar sperm cells. Agilent Seahorse technology was used assess both oxygen consumption rate (OCR), as index, extracellular acidification (ECAR), a index. Different metabolic parameters were studied on freshly ejaculated cells (identified day zero sample, d0) after...
The bergamot polyphenolic fraction (BPF) was evaluated in the F1FO-ATPase activity of swine heart mitochondria. In presence a concentration higher than 50 µg/mL BPF, ATPase F1FO-ATPase, dependent on natural cofactor Mg2+, increased by 15%, whereas enzyme Ca2+ inhibited 10%. By considering this opposite BPF effect, involved providing ATP synthesis oxidative phosphorylation and triggering mitochondrial permeability transition pore (mPTP) formation has been evaluated. improved catalytic...
Abstract The mitochondrial permeability transition pore (mPTP), a high‐conductance channel triggered by sudden Ca 2+ concentration increase, is composed of the F 1 O ‐ATPase. Since mPTP opening leads to dysfunction, which feature many diseases, great pharmacological challenge find modulators. In our study, effects two 1,5‐disubstituted 1,2,3‐triazole derivatives, five‐membered heterocycles with three nitrogen atoms in ring and capable forming secondary interactions proteins, were...
The compromised viability and function of cardiovascular cells are rescued by small molecules triazole derivatives (Tzs), identified as 3a 3b, preventing mitochondrial dysfunction. oxidative phosphorylation improves the respiratory control rate in presence Tzs independently substrates that energize mitochondria. F1FO-ATPase, main candidate permeability transition pore (mPTP) formation, is biological target hydrophilic F1 domain enzyme depicted binding region Tzs. protective effect Tz on...
The beneficial effects of bergamot polyphenolic fraction (BPF) on the mitochondrial bioenergetics porcine aortic endothelial cells (pAECs) were verified under cardiotoxic action doxorubicin (DOX). cell viability pAECs treated for 24 h with different concentrations DOX was reduced by 50%, but negative effect reversed in presence increasing doses BPF (100 µg/mL and 200 BPF). An analysis protective toxic also carried out respiration. We observed inhibition activity at 10 µM DOX, which not...