A5011854154- Autophagy in Disease and Therapy
- Adipokines, Inflammation, and Metabolic Diseases
- Electrospun Nanofibers in Biomedical Applications
- Muscle Physiology and Disorders
- S100 Proteins and Annexins
- Cardiomyopathy and Myosin Studies
- MicroRNA in disease regulation
- Inflammation biomarkers and pathways
- Diagnosis and Treatment of Venous Diseases
- Mitochondrial Function and Pathology
- Cancer-related molecular mechanisms research
- Neurological Disease Mechanisms and Treatments
- Barrier Structure and Function Studies
- Folate and B Vitamins Research
- interferon and immune responses
- ATP Synthase and ATPases Research
- Signaling Pathways in Disease
- Coronary Interventions and Diagnostics
- Adenosine and Purinergic Signaling
- Circular RNAs in diseases
- Venous Thromboembolism Diagnosis and Management
- Atherosclerosis and Cardiovascular Diseases
- Studies on Chitinases and Chitosanases
- Acute Myocardial Infarction Research
- Immune Response and Inflammation
Istituto Neurologico Mediterraneo
2017-2024
Bambino Gesù Children's Hospital
1993
Abstract Aims Low-grade endotoxaemia is detectable in human circulation but its role thrombosis still unclear. Methods and results We measured serum lipopolysaccharide (LPS) concentration, soluble P-selectin (sP-selectin), a marker of platelet activation, zonulin, gut permeability, peripheral circulation, coronary thrombi, intracoronary blood patients with ST-elevation myocardial infarction (STEMI, n = 50) stable angina (SA) (n 50), respectively, controls 50). Experimental study was carried...
The identification of the mechanisms predisposing to stroke may improve its preventive and therapeutic strategies in patients with essential hypertension. role macroautophagy/autophagy development hypertension-related needs be clarified. We hypothesized that a defective autophagy favor spontaneous by promoting mitochondrial dysfunction. studied stroke-prone spontaneously hypertensive (SHRSP) rat, which represents clinically relevant model associated high blood pressure. assessed autophagy,...
Abstract Aims Here, we aimed to determine the therapeutic effect of longevity-associated variant (LAV)-BPIFB4 gene therapy on atherosclerosis. Methods and results ApoE knockout mice (ApoE−/−) fed a high-fat diet were randomly allocated receive LAV-BPIFB4, wild-type (WT)-BPIFB4, or empty vector via adeno-associated viral injection. The primary endpoints study assess (i) vascular reactivity (ii) atherosclerotic disease severity, by Echo-Doppler imaging, histology ultrastructural analysis....
NPPA/atrial natriuretic peptide (natriuretic type A) exerts critical pleiotropic effects in the cardiovascular system, limiting cardiomyocyte hypertrophy and death, reducing cardiac fibrosis promoting vascular integrity. However, molecular mechanisms underlying these beneficial still need to be clarified. We demonstrated for first time that macroautophagy/autophagy is involved local protective of NPPA cardiomyocytes (CMs), both vitro vivo. Exogenous rapidly activates autophagy CMs through...
Abstract Beyond well-assessed risk factors, cardiovascular events could be also associated with the presence of epigenetic and genetic alterations, such as methylenetetrahydrofolate-reductase ( MTHFR ) C677T polymorphism. This gene variant is related to increased circulating levels homocysteine (Hcy) risk. However, heterozygous carriers have an augmented accidents independently from normal Hcy levels, suggesting additional deregulated processes in carriers. Here, we hypothesize that...
The compromised viability and function of cardiovascular cells are rescued by small molecules triazole derivatives (Tzs), identified as 3a 3b, preventing mitochondrial dysfunction. oxidative phosphorylation improves the respiratory control rate in presence Tzs independently substrates that energize mitochondria. F1FO-ATPase, main candidate permeability transition pore (mPTP) formation, is biological target hydrophilic F1 domain enzyme depicted binding region Tzs. protective effect Tz on...
Background The role of microRNAs dysregulation in tobacco cigarette smoking-induced vascular damage still needs to be clarified. We assessed the acute effects smoking on endothelial cell-related circulating healthy subjects. In addition, we investigated potential smoking-dependent cell damage. Methods and Results A panel endothelial-related was quantified subjects before after 1 cigarette. Serum levels miR-155 were found significantly increased shortly smoking. also observed a progressive...
Endothelial dysfunction contributes significantly to the development of vascular diseases. However, a therapy able reduce this derangement still needs be identified. We evaluated effects pharmacological inhibition Rac1, small GTPase protein promoting oxidative stress, in human endothelial dysfunction.We performed reactivity studies test NSC23766, Rac1 inhibitor, on endothelium-dependent vasorelaxation saphenous vein segments collected from 85 subjects who had undergone surgery for venous...
Atrial natriuretic peptide (ANP), a cardiac hormone involved in the regulation of water/sodium balance and blood pressure, is also secreted by endothelial cells, where it exerts protective effects response to stress. Autophagy an intracellular self-renewal process degradation dysfunctional cytoplasmic elements. ANP was recently reported act as extracellular regulator autophagy. However, its role autophagy has never been investigated. Here, we tested human umbilical vein cells (HUVECs). We...
The adult heart displays poor reparative capacities after injury. Cell transplantation and tissue engineering approaches have emerged as possible therapeutic options. Several stem cell populations been largely used to treat the infarcted myocardium. Nevertheless, transplanted cells displayed limited ability establish functional connections with host cardiomyocytes. In this study, we provide a new experimental tool, named 3D eX vivo muscle engineered (X-MET), define contribution of mechanical...
Cardiac stromal cells (CSCs) embrace multiple phenotypes and are a contributory factor in tissue homeostasis repair. They can be exploited as therapeutic mediators against cardiac fibrosis remodeling, but their survival cardioprotective properties decreased by microenvironmental cues. We evaluated the impact of autophagy modulation different pharmacological/genetic approaches on viability phenotype murine CSCs, which had been subjected to nutrient deprivation or hyperglycemia, order mimic...