A5065505549- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Neurogenetic and Muscular Disorders Research
- Genetic Neurodegenerative Diseases
- Amyotrophic Lateral Sclerosis Research
- Mesenchymal stem cell research
- Autophagy in Disease and Therapy
- Cardiac electrophysiology and arrhythmias
- Exercise and Physiological Responses
- Ion channel regulation and function
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Cardiomyopathy and Myosin Studies
- Tissue Engineering and Regenerative Medicine
- Caveolin-1 and cellular processes
- Nerve injury and regeneration
- RNA Research and Splicing
- Renin-Angiotensin System Studies
- Telomeres, Telomerase, and Senescence
- Mitochondrial Function and Pathology
- Extracellular vesicles in disease
- Muscle metabolism and nutrition
- Cancer, Hypoxia, and Metabolism
- Nutrition and Health in Aging
- Neuroscience and Neural Engineering
Sapienza University of Rome
2013-2025
Fondazione Santa Lucia
2014-2024
Istituto Pasteur
2022-2024
European University of Rome
2024
Istituti di Ricovero e Cura a Carattere Scientifico
2011-2022
Università Cattolica del Sacro Cuore
2020
Institut de Myologie
2018
Institute of Cell Biology and Neurobiology
2015-2016
National Research Council
2015
Sanford Burnham Prebys Medical Discovery Institute
2014-2015
Abstract Fibro-adipogenic progenitors (FAPs) are currently defined by their anatomical position, expression of non-specific membrane-associated proteins, and ability to adopt multiple lineages in vitro. Gene analysis at single-cell level reveals that FAPs undergo dynamic transitions through a spectrum cell states can be identified differential levels Tie2 Vcam1. Different patterns Vcam1-negative high or low /Vcam1-expressing detected during neonatal myogenesis, response acute injury Duchenne...
Fibro-adipogenic progenitors (FAPs) are important components of the skeletal muscle regenerative environment. Whether FAPs support regeneration or promote fibro-adipogenic degeneration is emerging as a key determinant in pathogenesis muscular diseases, including Duchenne dystrophy (DMD). However, molecular mechanism that controls FAP lineage commitment and activity currently unknown. We show here an HDAC–myomiR–BAF60 variant network regulates fate dystrophic muscles mdx mice. Combinatorial...
Patients with ALS show, in addition to the loss of motor neurons spinal cord, brainstem, and cerebral cortex, an abnormal depletion energy stores alongside hypermetabolism. In this study, we show that bioenergetic defects muscle remodeling occur skeletal SOD1G93A mouse model mice prior disease onset before activation denervation markers, respectively. These changes physiology were followed by increase expenditure unrelated physical activity. Finally, chronic treatment Ranolazine,...
We performed scRNA-seq/snATAC-seq of skeletal muscles post sciatic nerve transection to delineate cell type-specific patterns gene expression/chromatin accessibility at different time points post-denervation. Unlike myotrauma, denervation selectively activates glial cells and Thy1/CD90-expressing mesenchymal cells. Glial expressed Ngf receptor (Ngfr) were located near neuromuscular junctions (NMJs), close cells, which provided the main cellular source NGF Functional communication between...
Skeletal muscle remodeling in response to disuse and unloading is known be associated with so-called ER stress, which, turn, activates autophagy contributes atrophy. Different molecules are involved stress-induced autophagy, among which PKCθ has recently been described. In this study, we dissected both vitro vivo pathways muscle. Using C2C12 cells culture, demonstrated that PKC activation induced the absence of stress. We further was strongly activated cultured myoblasts myotubes during...
Background: Amyotrophic lateral sclerosis (ALS) is a fatal neuromuscular disease characterized by the degeneration of motor neurons that leads to muscle wasting and atrophy. Epidemiological experimental evidence suggests causal relationship between ALS physical activity (PA). However, impact PA on neuron loss sarcopenia still debated, probably because heterogeneity intensities proposed exercises. With this study, we aimed clarify effect intense endurance exercise onset progression in...
Inflammation is a key pathological characteristic of dystrophic muscle lesion formation, limiting regeneration and resulting in fibrotic fatty tissue replacement muscle, which exacerbates the wasting process muscles. Limiting immune response thus one therapeutic options to improve healing, as well efficacy gene- or cell-mediated strategies restore dystrophin expression. Protein kinase C θ (PKCθ) member PKCs family highly expressed both cells skeletal muscle; given its crucial role adaptive,...
Abstract Cardiovascular diseases (CVDs) are a major burden on the healthcare system: indeed, over two million new cases diagnosed every year worldwide. Unfortunately, important drawbacks for treatment of these patients derive from our current inability to stop structural alterations that lead heart failure, common endpoint many CVDs. In this scenario, better understanding role epigenetics – hereditable changes chromatin do not alter DNA sequence itself is warranted. To date, hyperacetylation...
Satellite cells (SCs) are muscle stem that remain quiescent during homeostasis and activated in response to acute damage or chronic degenerative conditions such as Duchenne Muscular Dystrophy. The activity of SCs is supported by specialized which either reside the recruited regenerating skeletal muscles, for instance macrophages (MΦs). By using a dystrophic mouse model transient MΦ depletion, we describe shift identity dependent on crosstalk between MΦs SCs. Indeed depletion determines...
Here, we report on the identification of Itga7-expressing muscle-resident glial cells activated by loss neuromuscular junction (NMJ) integrity. Gene expression analysis at bulk and single-cell level revealed that these are distinct from muscle satellite cells. We show a selective activation expansion Itga7+ occur in response to nerve lesion. Upon activation, glial-derived progenies expressed neurotrophic genes, including growth factor receptor, which enables their isolation FACS. also genes...
Age-related reduction in muscle stem cell (MuSC) regenerative capacity is associated with cell-autonomous and non–cell-autonomous changes caused by alterations systemic skeletal environments, ultimately leading to a decline MuSC number function. Previous studies demonstrated that STAT3 plays key role driving expansion differentiation after injury-activated regeneration, regulating autophagy activated MuSCs. However, gradually declines MuSCs during lifespan contributes the impairment of...
This study intended to demonstrate that the thyroid hormone T3 counteracts onset of a Streptozotocin (STZ) induced diabetes in wild type mice. To test our hypothesis has been Balb/c male mice by multiple low dose injection; and group was contemporaneously injected with T3. After 48 h were tested for glucose tolerance test, insulin serum levels then sacrified. Whole pancreata utilized morphological biochemical analyses, while protein extracts RNA expression analyses specific molecules. The...
Fusion of mononucleated myoblasts to form multinucleated myofibers is an essential phase skeletal myogenesis, which occurs during muscle development as well postnatal life for growth, turnover, and regeneration. Many cell adhesion proteins, including integrins, have been shown be important myoblast fusion in vertebrates, recently focal kinase (FAK), has proposed a key mediator fusion. Here we focused on the possible role PKC, PKC isoform predominantly expressed muscle, We found that...
Angiotensin (1-7) production increases during AT1R (angiotensin type-1 receptor) blockade. The contribution of Ang [1-7]) and its receptor (MasR) to the favorable effect angiotensin blockers on remodeling function resistance arteries remains unclear. We sought determine whether MasR contributes improvement vascular structure chronic Spontaneously hypertensive rats were treated with or olmesartan ± antagonist A-779, vehicle, for 14 days. Blood pressure was measured by tail cuff methodology....