A5074705768- RNA Research and Splicing
- Amyotrophic Lateral Sclerosis Research
- Neurogenetic and Muscular Disorders Research
- Advanced Fluorescence Microscopy Techniques
- Muscle Physiology and Disorders
- Autophagy in Disease and Therapy
- Extracellular vesicles in disease
- Advanced Optical Sensing Technologies
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Nerve injury and regeneration
- RNA modifications and cancer
- Ocular Infections and Treatments
- Intracerebral and Subarachnoid Hemorrhage Research
- Histone Deacetylase Inhibitors Research
- MicroRNA in disease regulation
- Metalloenzymes and iron-sulfur proteins
- Cell Image Analysis Techniques
- Heat shock proteins research
- Biotin and Related Studies
- RNA and protein synthesis mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Lipid Membrane Structure and Behavior
- Genetic Neurodegenerative Diseases
Italian Institute of Technology
2020-2025
Sapienza University of Rome
2020
Istituto di Farmacologia Traslazionale
2017
National Academies of Sciences, Engineering, and Medicine
2017
Fondazione Santa Lucia
2016-2017
Istituti di Ricovero e Cura a Carattere Scientifico
2016
Biomolecular condensates serve as membrane-less compartments within cells, concentrating proteins and nucleic acids to facilitate precise spatial temporal orchestration of various biological processes. The diversity these processes the substantial variability in condensate characteristics present a formidable challenge for quantifying their molecular dynamics, surpassing capabilities conventional microscopy. Here, we show that our single-photon microscope provides comprehensive live-cell...
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease due to gradual motoneurons (MN) degeneration. Among the processes associated ALS pathogenesis, there formation of cytoplasmic inclusions produced by aggregation mutant proteins, among which RNA binding protein FUS. Here we show that, in neuronal cells and iPSC-derived MN expressing FUS, such are significantly reduced number dissolve faster when m
Lately, important advancements in visualizing RNAs fixed and live cells have been achieved. While mRNA imaging techniques are well-established, the development of effective methods for studying non-coding (ncRNAs) living still challenging but necessary, as they show a variety functions intracellular localizations, including participation highly dynamic processes like phase-transition, which is poorly studied vivo. Addressing this issue, we tagged two exemplary ncRNAs with fluorescent RNA...
Abstract Single-particle tracking techniques enable investigation of the complex functions and interactions individual particles in biological environments. Many such exist, each demonstrating trade-offs between spatiotemporal resolution, spatial temporal range, technical complexity, information content. To mitigate these trade-offs, we enhanced a confocal laser scanning microscope with an asynchronous read-out single-photon avalanche diode array detector. This detector provides image...
ABSTRACT Stress Granules (SG) formation is a cellular protection mechanism, constituting storage for untranslated mRNAs and RNA-binding proteins (RBPs); however, these condensates can turn into pathological aggregates, related to the onset of neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS). This transition towards cytotoxic inclusions triggered by ALS-causative mutations in RBP FUS, which lead its cytoplasmic mis-localization accumulation SG. Here, we describe SG...
Abstract Stress granules (SG) are part of a cellular protection mechanism where untranslated messenger RNAs and RNA-binding proteins stored upon conditions stress. Compositional variations due to qualitative or quantitative protein changes can disrupt their functionality alter structure. This is the case different forms amyotrophic lateral sclerosis (ALS) causative link has been proposed between cytoplasmic de-localization mutant proteins, such as FUS (Fused in Sarcoma), formation cytotoxic...
Article29 June 2020Open Access Source DataTransparent process Trans-generational epigenetic regulation associated with the amelioration of Duchenne Muscular Dystrophy Julie Martone Department Biology and Biotechnology, 'Charles Darwin', Sapienza University Rome, Italy CNR Institute Molecular Pathology (IBPM), Search for more papers by this author Michela Lisi Francesco Castagnetti Center Human Technologies, Istituto Italiano di Tecnologia, Genova, Alessandro Rosa...
ABSTRACT Lately, important advancements in visualizing RNAs fixed and live cells have been achieved. While mRNA imaging techniques are well-established, the development of effective methods for studying non-coding (ncRNAs) living still challenging but necessary, as they cover a variety function intracellular localization, including highly dynamic processes like phase-transition, poorly studied vivo . Addressing this issue, we tagged two exemplary ncRNAs with innovative fluorescent RNA (fRNA)...
Abstract Quantitative fluorescence microscopy is experiencing an important revolution thanks to single-photon array detectors. These detectors provide users with so far inaccessible specimen information: The distribution of the specimen’s emission at level and high spatiotemporal sampling. In laser-scanning microscopy, this photon-resolved measurement has enabled robust lifetime imaging sub-diffraction spatial resolution, thus opening new perspectives for structural functional imaging....
Abstract Single-particle tracking (SPT) techniques are essential for investigating the com-plex functions and interactions of individual, specifically labelled particles in biological environments. Many SPT exist, each optimised towards a different balance between spatiotemporal resolution range, technical com-plexity, information content. This bargain is exemplified by contrast wide-field camera-based real-time approaches, with latter being generally more advanced but at cost high...
Abstract Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease due to gradual motorneurons (MN) degeneration 1 . Among the processes associated ALS pathogenesis, there formation of cytoplasmic inclusions produced by mutant protein aggregation, among which RNA binding FUS 2 In this work we show that such are significantly reduced in number and dissolve faster when m 6 A content diminished as consequence writer METTL3 knock-down. These effects were observed both...
Increasing evidence points to autophagy as a crucial regulatory process preserve tissue homeostasis. It is known that involved in skeletal muscle development and regeneration, the autophagic has been described several muscular pathologies age-related disorders. A recently block of correlates with functional exhaustion satellite cells during repair supports notion active coupled productive regeneration. These data uncover role cell activation regeneration both normal pathological conditions,...
Increasing evidence points to autophagy as a crucial regulatory process preserve tissue homeostasis. It is known that involved in skeletal muscle development and regeneration, the autophagic has been described several muscular pathologies age-related disorders. A recently block of correlates with functional exhaustion satellite cells during repair supports notion active coupled productive regeneration. These data uncover role cell activation regeneration both normal pathological conditions,...