A5053037511- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Genomics and Chromatin Dynamics
- Neurogenetic and Muscular Disorders Research
- DNA and Nucleic Acid Chemistry
- Pluripotent Stem Cells Research
- Amyotrophic Lateral Sclerosis Research
- Epigenetics and DNA Methylation
- RNA regulation and disease
- DNA Repair Mechanisms
- Receptor Mechanisms and Signaling
- Chemokine receptors and signaling
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Hedgehog Signaling Pathway Studies
- Photosynthetic Processes and Mechanisms
- thermodynamics and calorimetric analyses
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- Histone Deacetylase Inhibitors Research
National Research Council
2025
Institute of Molecular Biology and Pathology
2010-2023
National Research Council
2005-2021
Sapienza University of Rome
2004-2018
Italian Institute of Technology
2012-2017
National Academies of Sciences, Engineering, and Medicine
2015
Nanobiotix (France)
2014
Istituto Pasteur
1997-2010
Institut Pasteur
2003
Consorzio Roma Ricerche
1992-2002
Abstract The RNA-binding protein FUS participates in several RNA biosynthetic processes and has been linked to the pathogenesis of amyotrophic lateral sclerosis (ALS) frontotemporal dementia. Here we report that controls back-splicing reactions leading circular (circRNA) production. We identified circRNAs expressed vitro -derived mouse motor neurons (MNs) determined production a considerable number these is regulated by FUS. Using RNAi overexpression wild-type ALS-associated mutants,...
Abstract Medulloblastoma is an aggressive brain malignancy with high incidence in childhood. Current treatment approaches have limited efficacy and severe side effects. Therefore, new risk‐adapted therapeutic strategies based on molecular classification are required. MicroRNA expression analysis has emerged as a powerful tool to identify candidate molecules playing important role large number of malignancies. However, no data yet available human primary medulloblastomas. A throughput...
MicroRNAs (miRNAs) are tiny noncoding RNAs whose function as modulators of gene expression is crucial for the proper control cell growth and differentiation. Although profile miRNA has been defined many different cellular systems, elucidation regulatory networks in which they involved only just emerging. In this work, we identify a role three neuronal miRNAs (9, 125a, 125b) controlling human neuroblastoma proliferation. We show that these molecules act an additive manner by repressing common...
miRNAs play key roles in the nervous system, where they mark distinct developmental stages. Accordingly, dysregulation of miRNA expression may have profound effects on neuronal physiology and pathology, including cancer. Among miRNAs, miR-9 was shown to be upregulated during vitro differentiation downregulated 50% primary neuroblastoma tumors, suggesting a potential function as an oncosuppressor gene. In this study we characterized promoter transcriptional regulation miR-9-2 gene...
The transcription factor ID2 is an important repressor of neural differentiation strongly implicated in nervous system cancers. MicroRNAs (miRNAs) are increasingly involved control and cancer development. Here we show that two miRNAs upregulated on neuroblastoma cells – miR-9 miR-103 restrain expression by directly targeting the coding sequence 3′ untranslated region encoding messenger RNA, respectively. Notably, inverse correlation with during cell induced retinoic acid. Overexpression...
Here we report the purification, fromXenopus laevis oocyte nuclear extracts, of a new endoribonuclease, XendoU, that is involved in processing intron-encoded box C/D U16 small nucleolar RNA (snoRNA) from its host pre-mRNA. Such an activity has never been reported before and several uncommon features make it quite novel enzyme: poly(U)-specific, requires Mn2+ ions, produces molecules with 2′-3′-cyclic phosphate termini. Even if XendoU cleaves U-stretches, displays some preferential cleavage...
Mutations in fused sarcoma (FUS) cause amyotrophic lateral sclerosis (ALS). FUS is a multifunctional protein involved the biogenesis and activity of several types RNAs, its role pathogenesis ALS may involve both direct effects disease-associated mutations through gain- loss-of-function mechanisms indirect due to cross talk between different classes FUS-dependent RNAs. To explore how impinge on motor neuron-specific RNA-based circuitries, we performed transcriptome profiling small long RNAs...
// Pietro Laneve 1 , Agnese Po 2 Annarita Favia 3 Ivano Legnini 4 Vincenzo Alfano 1, Jessica Rea Valerio Di Carlo 4, 11 Valeria Bevilacqua 12 Evelina Miele 13 Angela Mastronuzzi 5 Andrea Carai 6 Franco Locatelli 5, 7 Irene Bozzoni 3, 8 Elisabetta Ferretti 9, 10 Elisa Caffarelli Center for Life NanoScience@Sapienza, Istituto Italiano di Tecnologia, 00161 Rome, Italy Department of Molecular Medicine, Sapienza University Institute Biology and Pathology, National Research Council, 00185...
Neuronal differentiation is a timely and spatially regulated process, relying on precisely orchestrated gene expression control. The sequential activation/repression of genes driving cell fate specification achieved by complex regulatory networks, where transcription factors noncoding RNAs work in coordinated manner. Herein, we identify the long RNA HOTAIRM1 (HOXA Transcript Antisense RNA, Myeloid-Specific 1) as new player neuronal differentiation. We demonstrate that neuronal-enriched...
Lately, important advancements in visualizing RNAs fixed and live cells have been achieved. While mRNA imaging techniques are well-established, the development of effective methods for studying non-coding (ncRNAs) living still challenging but necessary, as they show a variety functions intracellular localizations, including participation highly dynamic processes like phase-transition, which is poorly studied vivo. Addressing this issue, we tagged two exemplary ncRNAs with fluorescent RNA...
It was recently shown that a new class of small nuclear RNAs is encoded in introns protein-coding genes and they originate by processing the pre-mRNA which are contained. Little known about mechanism factors involved this type processing. The L1 ribosomal protein gene Xenopus laevis well-suited system for studying phenomenon: several different encode two nucleolar (snoRNAs; U16 U18). In paper, we analyzed vitro these snoRNAs showed both released from common mechanism: endonucleolytic...
Human PP11 (placental protein 11) was previously described as a serine protease specifically expressed in the syncytiotrophoblast and numerous tumor tissues. Several PP11-like proteins were annotated distantly related organisms, such worms mammals, suggesting their involvement evolutionarily conserved processes. Based on sequence similarity, human included family whose characterized members are XendoU, Xenopus laevis endoribonuclease involved small nucleolar RNA processing, Nsp15, an...
Long non-coding RNAs (lncRNAs) are currently recognized as crucial players in nervous system development, function and pathology. In Amyotrophic Lateral Sclerosis (ALS), identification of causative mutations FUS TDP-43 or hexanucleotide repeat expansion C9ORF72 point to the essential role aberrant RNA metabolism neurodegeneration. this study, by taking advantage an vitro differentiation generating mouse motor neurons (MNs) from embryonic stem cells, we identified characterized long...
XendoU is the endoribonuclease involved in biosynthesis of a specific subclass Xenopus laevis intron-encoded small nucleolar RNAs. has no homology to any known cellular RNase, although it sequence similarity with proteins tentatively annotated as serine proteases. It been recently shown that represents counterpart nidovirus replicative (NendoU), which plays critical role viral replication and transcription. In this paper, we combined prediction experimental data define amino acid residues...
Small nucleolar RNAs (snoRNAs) play a key role in eukaryotic ribosome biogenesis. In most cases, snoRNAs are encoded introns and released through the splicing reaction. Some are, instead, produced by an alternative pathway consisting of endonucleolytic processing pre-mRNA. XendoU, endoribonuclease responsible for this activity, is U-specific, metal-dependent enzyme that releases products with 2′–3′ cyclic phosphate termini. XendoU broadly conserved among eukaryotes, it genetic marker...
Musashi1 is an RNA binding protein that controls the neural cell fate, being involved in maintaining progenitors their proliferative state. In particular, its downregulation needed for triggering early differentiation programs. this study, we profiled microRNA expression during transition from to differentiated astrocytes and underscored 2 upregulated microRNAs, miR-23a miR-125b, sinergically act restrain expression, thus creating a regulatory module controlling progenitor proliferation.
Glioblastoma (GBM) is the most aggressive human brain tumor. The high growth potential and decreased susceptibility to apoptosis of glioma cells mainly dependent on genetic amplifications or mutations oncogenic pro-apoptotic genes, respectively. We have previously shown that activation M2 acetylcholine muscarinic receptors inhibited cell proliferation induced in two GBM lines cancer stem cells. aim this study was delve into molecular mechanisms underlying M2-mediated arrest. Exploiting U87MG...
Glioblastomas (GBM) are the most aggressive form of primary brain tumors in humans. A key feature malignant gliomas is their cellular heterogeneity. In particular, presence an undifferentiated cell population defined Glioblastoma Stem cells (GSCs) was reported. Increased expression anti-apoptotic and chemo-resistance genes GCSs subpopulation favors high resistance to a broad spectrum drugs. Our previous studies showed ability M2 muscarinic receptors negatively modulate growth GBM lines GSCs....