A5044346256- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- Neuroblastoma Research and Treatments
- Genomics and Chromatin Dynamics
- Immune Cell Function and Interaction
- Chromatin Remodeling and Cancer
- Microtubule and mitosis dynamics
- Glioma Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
- Cancer-related gene regulation
- Genetics and Neurodevelopmental Disorders
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- HER2/EGFR in Cancer Research
- RNA modifications and cancer
- Polyamine Metabolism and Applications
- Developmental Biology and Gene Regulation
- TGF-β signaling in diseases
- Oral and Maxillofacial Pathology
- Genomic variations and chromosomal abnormalities
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Protein Degradation and Inhibitors
- RNA Research and Splicing
Sapienza University of Rome
2015-2024
Istituto Pasteur
2015-2024
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2017
Italian Institute of Technology
2015-2016
Center for Life Sciences
2015
University of L'Aquila
1997-2004
New York University
2001-2002
Abstract Medulloblastoma is an aggressive brain malignancy with high incidence in childhood. Current treatment approaches have limited efficacy and severe side effects. Therefore, new risk‐adapted therapeutic strategies based on molecular classification are required. MicroRNA expression analysis has emerged as a powerful tool to identify candidate molecules playing important role large number of malignancies. However, no data yet available human primary medulloblastomas. A throughput...
Estrogen receptor (ER) expression and Her-2 amplification define specific subsets of breast tumors for which therapies exist. The S-phase kinase-associated protein Skp2 is required the ubiquitin-mediated degradation cdk-inhibitor p27 a bona fide proto-oncoprotein. Using microarray analysis immunohistochemistry, we determined that higher levels are present more frequently in ER-negative than ER-positive cases. Interestingly, subset carcinomas overexpressing also characterized by high tumor...
Estrogen receptor (ER) expression and Her-2 amplification define specific subsets of breast tumors for which therapies exist. The S-phase kinase-associated protein Skp2 is required the ubiquitin-mediated degradation cdk-inhibitor p27 a bona fide proto-oncoprotein. Using microarray analysis immunohistochemistry, we determined that higher levels are present more frequently in ER-negative than ER-positive cases. Interestingly, subset carcinomas overexpressing also characterized by high tumor...
Hedgehog signaling is suggested to be a major oncogenic pathway in medulloblastoma, which arises from aberrant development of cerebellar granule progenitors. Allelic loss chromosome 17p has also been described as the most frequent genetic defect this human neoplasia. This observation raises question possible interplay between deletion and tumorigenic pathway. Here, we identify orthologue mouse REN(KCTD11), previously reported expressed differentiating low proliferating neuroblasts. Human...
MicroRNAs (miRNAs) are tiny noncoding RNAs whose function as modulators of gene expression is crucial for the proper control cell growth and differentiation. Although profile miRNA has been defined many different cellular systems, elucidation regulatory networks in which they involved only just emerging. In this work, we identify a role three neuronal miRNAs (9, 125a, 125b) controlling human neuroblastoma proliferation. We show that these molecules act an additive manner by repressing common...
Article4 December 2014Open Access Source Data Gli1/DNA interaction is a druggable target for Hedgehog-dependent tumors Paola Infante Center Life [email protected], Istituto Italiano di Tecnologia, Rome, Italy Search more papers by this author Mattia Mori Romina Alfonsi Department of Molecular Medicine, University La Sapienza, Francesca Ghirga Dipartimento Chimica e Tecnologie del Farmaco, Federica Aiello Chemistry and Industrial Chemistry, Pisa, Sara Toscano Cinzia Ingallina Mariangela Siler...
Aberrant Hedgehog/GLI signaling has been implicated in a diverse spectrum of human cancers, but its role lung adenocarcinoma (LAC) is still under debate. We show that the downstream effector Hedgehog pathway, GLI1, expressed 76% LACs, roughly half these tumors, canonical pathway activator, Smoothened, at low levels, possibly owing to epigenetic silencing. In LAC cells including cancer stem cell compartment, we GLI1 activated noncanonically by MAPK/ERK signaling. Different mechanisms can...
Medulloblastoma is the most common pediatric malignant brain tumor, arising from aberrant cerebellar precursors' development, a process mainly controlled by Hedgehog (Hh) signaling pathway. Histone deacetylase HDAC1 has been recently shown to modulate Hh signaling, deacetylating its effectors Gli1/2 and enhancing their transcriptional activity. Therefore, HDAC may represent potential therapeutic target for Hh-dependent tumors, but still little information available on physiological...
We synthesized 3-aroyl-1-arylpyrrole (ARAP) derivatives as potential anticancer agents having different substituents at the pendant 1-phenyl ring. Both ring and 3-(3,4,5-trimethoxyphenyl)carbonyl moieties were mandatory to achieve potent inhibition of tubulin polymerization, binding colchicine tubulin, cancer cell growth. ARAP 22 showed strong P-glycoprotein-overexpressing NCI-ADR-RES Messa/Dx5MDR lines. Compounds 27 suppressed in vitro Hedgehog signaling pathway, strongly reducing...
ABSTRACT Eukaryotic Translation Initiation Factor 5A (EIF5A) is a translation factor regulated by hypusination, unique posttranslational modification catalyzed deoxyhypusine synthetase (DHPS) and hydroxylase (DOHH) starting from the polyamine spermidine. Emerging data are showing that hypusinated EIF5A regulates key cellular processes such as autophagy, senescence, homeostasis, energy metabolism, plays role in cancer. However, effects of inhibition preclinical cancer models, mechanism...
Glioblastoma multiforme (GB) is the most malignant primary brain tumor in humans, with an overall survival of approximatively 15 months. The molecular heterogeneity GB, as well its rapid progression, invasiveness and occurrence drug-resistant cancer stem cells, limits efficacy current treatments. In order to develop innovative therapeutic strategy, it mandatory identify characterize new players responsible for GB phenotype. this study, RNA-binding ubiquitin ligase MEX3A was selected from a...
A key mechanism driving colorectal cancer (CRC) development is the upregulation of MYC and its targets, including ornithine decarboxylase (ODC), a master regulator polyamine metabolism. Elevated polyamines promote tumorigenesis in part by activating DHPS-mediated hypusination translation factor eIF5A, thereby inducing biosynthesis. Thus, MYC, ODC eIF5A orchestrate positive feedback loop that represents an attractive therapeutic target for CRC therapy. Here we show combined inhibition induces...
The morphogenic Hedgehog (Hh) signaling regulates postnatal cerebellar development and its aberrant activation leads to medulloblastoma. transcription factors Gli1 Gli2 are the activators of Hh pathway their function is finely controlled by different covalent modifications, such as phosphorylation ubiquitination. We show here that endogenously acetylated this modification represents a key regulatory step for signaling. histone acetyltransferase (HAT) coactivator p300, but not other HATs,...