A5007452991- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Nerve injury and regeneration
- Cholinesterase and Neurodegenerative Diseases
- Vagus Nerve Stimulation Research
- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Cancer, Stress, Anesthesia, and Immune Response
- Ion channel regulation and function
- Immune Cell Function and Interaction
- Neuroscience and Neural Engineering
- Pain Mechanisms and Treatments
- MicroRNA in disease regulation
- Chemokine receptors and signaling
- Glioma Diagnosis and Treatment
- Multiple Sclerosis Research Studies
- Viral-associated cancers and disorders
- Neuroblastoma Research and Treatments
- Lipid Membrane Structure and Behavior
- Circular RNAs in diseases
- Biochemical and Structural Characterization
- Zebrafish Biomedical Research Applications
- Porphyrin Metabolism and Disorders
Sapienza University of Rome
2016-2025
Duke University
2025
University of Ferrara
2023
European University of Rome
2022
Centre National de la Recherche Scientifique
2020
Université Paris Sciences et Lettres
2020
Institut Curie
2020
Bentham Science Publishers (United Arab Emirates)
2014
Bentham Science Publishers (China)
2014
University of Chieti-Pescara
2013
Vitamin B12 (VitB12) is a micronutrient and acts as cofactor for fundamental biochemical reactions: the synthesis of succinyl-CoA from methylmalonyl-CoA biotin, methionine folic acid homocysteine. VitB12 deficiency can determine wide range diseases, including nervous system impairments. Although clinical evidence shows direct role in neuronal homeostasis, molecular mechanisms are yet to be characterized depth. Earlier investigations focused on exploring shifts resulting function coenzyme,...
In recent years a growing debate is about whether botulinum neurotoxins are retrogradely transported from the site of injection. Immunodetection cleaved SNAP-25 (cl-SNAP-25), protein SNARE complex targeted by neurotoxin serotype A (BoNT/A), could represent an excellent approach to investigate mechanism action on nociceptive pathways at peripheral and/or central level. After administration BoNT/A, we analyzed expression cl-SNAP-25, hindpaw's nerve endings spinal cord, together with behavioral...
Acetylcholine (ACh) is a major neurotransmitter but also an important signaling molecule in neuron-glia interactions. Expression of ACh receptors has been reported several glial cell populations, including oligodendrocytes (OLs). Nonetheless, the characterization muscarinic these cells, as well description cholinergic effects at different stages OL development, still incomplete. In this study, we characterized pattern expression receptor subtypes primary cultures rat oligodendrocyte...
Multiple sclerosis (MS) is a chronic inflammatory, demyelinating and neurodegenerative disorder. Since acetylcholine (ACh) known to participate in the inflammatory response, we investigated possible relationship between pro-inflammatory cytokines levels relapsing-remitting multiple (RR-MS) patients. Levels of ACh IL1-β IL-17 were measured both cerebrospinal fluid (CSF) sera 22 RR-MS patients relapsing phase 17 control subjects affected by other non-neurological diseases (OND). We...
Multiple Sclerosis (MS) is an autoimmune disease, having not fully understood aetiology, and both genetic environmental factors contribute to the pathogenesis of disease. The cholinergic system has been indicated as a mediator neuro-immune interactions, well internal regulator immune responses. aim present research was assess associations between BChE AChE variations serum inflammatory profiles in 102 Relapsing Remitting-MS patients 117 healthy controls. An increased frequency K-allele MS...
Growing evidence indicates that cell cycle arrest and neurogenesis are highly coordinated interactive processes, governed by genes neural transcription factors. The gene PC3 ( Tis21 / BTG2 ) is expressed in the neuroblast throughout tube inhibits progression at G 1 checkpoint repressing cyclin D1 transcription. We generated inducible mouse models which expression of was upregulated neuronal precursors cerebellum. These mice exhibited a marked increase production postmitotic neurons...
Glioblastoma cells in vivo are exposed to a variety of promigratory signals, including undefined serum components that infiltrate into high grade gliomas as result blood-brain barrier breakdown. cell migration has been further shown depend heavily on ion channels activity. We have then investigated the modulatory effects fetal calf (FCS) channels, and their involvement U87-MG migration. Using perforated patch-clamp technique we found that, subpopulation (42%), FCS induced: (1) an oscillatory...
Abstract Muscarinic receptors, expressed in several primary and metastatic tumours, appear to be implicated their growth propagation. In this work we have demonstrated that M2 muscarinic receptors are glioblastoma human specimens cell lines. Moreover, characterized the effects of agonist arecaidine on survival both two different lines (U251 MG U87 ) cultures obtained from biopsies. Cell analysis has strongly decreased proliferation glioma cultures. This effect was dose time dependent. FACS...
Background: Oligodendrocytes (OLs) are highly sensitive to oxygen-reactive species produced during neuro-inflammation. α7 nAChRs expressed in glial cells where they can modulate several physiological and pathological functions. OL progenitors (OPCs) respond cholinergic stimuli via muscarinic receptors that mainly involved the modulation of their proliferation. Differently, role nicotinic receptors, particular nAChRs, has been poorly investigated. In this study, we evaluated expression a...
Human adipose-derived stem cells (ASCs), differentiated in vitro towards Schwann-like phenotype (hdASCs), have been suggested as an alternative source of Schwann (SCs). However, although they seem a good alternative, their differentiation is unstable, losing SC-like following growth factor withdrawal. Rat and human SCs rat dASCs characterized for acetylcholine M2 muscarinic receptor subtype that plays strategic role the regulation differentiation. Here, we evaluated activation controlling...
Abstract The expression of different muscarinic receptor subtypes was analyzed in immature Schwann cells obtained from sciatic nerve 2‐day neonatal rats. By using RT‐PCR analysis, we demonstrated the presence M1, M2, M3, and M4 cultured cells, with M2 displaying highest levels. Muscarinic were also quantified by immunoprecipitation [ 3 H]QNB binding. With this approach, found levels to be > M3 M1. is expressed at very low levels, M5 not detectable. Moreover, that stimulation receptors...
Glial cells express acetylcholine receptors. In particular, rat Schwann different muscarinic receptor subtypes, the most abundant of which is M2 subtype. activation causes a reversible arrest cell cycle. This negative effect on proliferation suggests that these may possibly progress into differentiating program. this study we analyzed in vitro modulation, by agonist arecaidine, transcription factors and specific signaling pathways involved differentiation. The arecaidine-induced...
The role of muscarinic receptors in several diseases including cancer has recently emerged. To evaluate the hypothesis that acetylcholine may play a bladder as well other tumor types, we investigated their expression specimens. All examined samples expressed M1, M2 and M3 receptor subtypes. We also found level transcripts, but not those M1 or M3, significantly increased with histologic grade. In view these results, proceeded to investigate whether agonist Arecaidine had any effect on vitro...