A5010398548- Botulinum Toxin and Related Neurological Disorders
- Pain Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Neurological disorders and treatments
- Hereditary Neurological Disorders
- Adipose Tissue and Metabolism
- Nerve injury and regeneration
- Photoreceptor and optogenetics research
- Cancer Treatment and Pharmacology
- Muscle Physiology and Disorders
- Ion channel regulation and function
- ATP Synthase and ATPases Research
- Nicotinic Acetylcholine Receptors Study
- Photosynthetic Processes and Mechanisms
- Migraine and Headache Studies
- Lipid Membrane Structure and Behavior
- Cardiac electrophysiology and arrhythmias
- Hemoglobin structure and function
- Biochemical effects in animals
- Spinal Cord Injury Research
- Anesthesia and Neurotoxicity Research
- Spectroscopy and Quantum Chemical Studies
- Fuel Cells and Related Materials
- Pharmacological Effects of Natural Compounds
- Electron Spin Resonance Studies
Institute of Cell Biology and Neurobiology
2013-2024
National Research Council
2012-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2018
Fondazione Santa Lucia
2014-2018
National Academies of Sciences, Engineering, and Medicine
2012-2015
Institute of Molecular Biology and Pathology
2015
Neuroscience Institute
2003-2010
Institute of Neurobiology and Molecular Medicine
2004-2009
University of Padua
1989-2004
Genomics Institute of the Novartis Research Foundation
2002
Mutations in α 1A , the pore-forming subunit of P/Q-type calcium channels, are linked to several human diseases, including familial hemiplegic migraine (FHM). We introduced four missense mutations FHM into 1A-2 subunits and investigated their functional consequences after expression embryonic kidney 293 cells. By combining single-channel whole-cell patch-clamp recordings, we show that all affect both biophysical properties density channels. Mutation R192Q S4 segment domain I increased...
Insights into the pathogenesis of migraine with aura may be gained from a study human Ca(V)2.1 channels containing mutations linked to familial hemiplegic (FHM). Here, we extend previous single-channel analysis mutation V1457L. This increased channel open probability by shifting its activation more negative voltages and reduced both unitary conductance density functional in membrane. To investigate possibility changes function common all FHM mutations, calculated product current as measure...
In recent years a growing debate is about whether botulinum neurotoxins are retrogradely transported from the site of injection. Immunodetection cleaved SNAP-25 (cl-SNAP-25), protein SNARE complex targeted by neurotoxin serotype A (BoNT/A), could represent an excellent approach to investigate mechanism action on nociceptive pathways at peripheral and/or central level. After administration BoNT/A, we analyzed expression cl-SNAP-25, hindpaw's nerve endings spinal cord, together with behavioral...
In experimental and clinical pain studies, the sex of subjects was rarely taken into account, even if nociceptive inputs appear to be processed modulated by partially distinct neural mechanisms in each sex. this study we analysed, male female mice, behavioural neuronal responses developing, maintaining, recovering from neuropathic pain. Experiments were carried out adult CD1 mice using Chronic Constriction Injury (CCI) as model. We investigated temporal trend mechanical threshold together...
Abstract Sex differences play a role in pain sensitivity, efficacy of analgesic drugs and prevalence neuropathic pain, even if the underlying mechanisms are far from being understood. We demonstrate that male female mice react differently to structural functional changes induced by sciatic nerve ligature, used as model pain. Male show gradual decrease allodynia complete recovery while, females, gliosis still present four months after neuropathy induction. Administration 17β-estradiol is able...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUncoupling of oxidative phosphorylation. 1. Protonophoric effects account only partially for uncouplingSiro Luvisetto, Daniela Pietrobon, and Giovanni Felice AzzoneCite this: Biochemistry 1987, 26, 23, 7332–7338Publication Date (Print):November 1, 1987Publication History Published online1 May 2002Published inissue 1 November 1987https://pubs.acs.org/doi/10.1021/bi00397a021https://doi.org/10.1021/bi00397a021research-articleACS PublicationsRequest...
Summary Processing of A β‐precursor protein ( APP ) plays an important role in lzheimer's disease AD pathogenesis. The intracellular domain contains residues regulating function and processing, particular the 682 YENPTY 687 motif. To dissect functions this sequence vivo , we created knock‐in allele mutating Y to G ly YG/YG mice). This mutation alters processing T rk signaling leads postnatal lethality neuromuscular synapse defects when expressed on ‐like 2 KO background. evidence prompted us...
Up-regulation of the dystrophin-related gene utrophin represents a promising therapeutic strategy for treatment Duchenne Muscular Dystrophy (DMD). In order to re-program expression level in muscle, we engineered artificial zinc finger transcription factors (ZF-ATFs) that target ʻA' promoter. We have previously shown ZF-ATF "Jazz", either by transgenic manipulation or systemic adeno-associated viral delivery, induces significant rescue muscle function dystrophic "mdx" mice. present full...
The mechanism of uncoupling oxidative phosphorylation by carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP), oleic acid, and chloroform is further investigated measuring in the presence a certain concentration each type uncoupler (i) mitochondrial P/O respiratory control ratios upon progressive inhibition redox pumps (ii) delta mu H rate either electron transfer or adenosine 5'-triphosphate (ATP) hydrolysis static head triphosphatase (ATPase) proton pumps. Chloroform exhibits all...
The absence of the cytoskeletal protein dystrophin results in Duchenne muscular dystrophy (DMD). utrophin is best candidate for replacement DMD patients. To obtain therapeutic levels expression dystrophic muscle, we developed an alternative strategy based on use artificial zinc finger transcription factors (ZF ATFs). ZF ATF 'Jazz' was recently engineered and tested vivo by generating a transgenic mouse specifically expressing Jazz at level. validate technology treatment generated second...
Response strategy in the dual-solution plus maze is regarded as a form of stimulus-response learning. In this study, by using an outcome devaluation procedure, we show that it can be based on both action-outcome and habit learning, depending amount training animals receive. Furthermore, deactivation dorso-medial dorso-lateral striatum with Botulinum neurotoxin A , mimicked or abolished, respectively, effects practice sensitivity response to devaluation. These findings have relevant...