A5051456213- Nerve injury and regeneration
- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Neurogenesis and neuroplasticity mechanisms
- Vagus Nerve Stimulation Research
- Axon Guidance and Neuronal Signaling
- Neuropeptides and Animal Physiology
- Mesenchymal stem cell research
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Infant Nutrition and Health
- RNA Interference and Gene Delivery
- Olfactory and Sensory Function Studies
- Pancreatic function and diabetes
- Ion channel regulation and function
- Protein Degradation and Inhibitors
- Sleep and Wakefulness Research
- Chemokine receptors and signaling
- Amyotrophic Lateral Sclerosis Research
- Neuroblastoma Research and Treatments
- Digestive system and related health
- Cannabis and Cannabinoid Research
- Congenital gastrointestinal and neural anomalies
- Adipose Tissue and Metabolism
Université de Montréal
2021-2025
Sapienza University of Rome
2017-2025
Manchester Academic Health Science Centre
2020-2025
University of Manchester
2020-2025
Charles Darwin University
2020
University of Milan
2017
Introdução: A Doença Inflamatória Intestinal de início precoce (VEO-DII) apresenta-se antes dos 6 anos idade, desencadeando processo inflamatório crônico no trato gastrointestinal. Dado seu precoce, o diagnóstico torna-se difícil. Objetivo: Relatar as dificuldades do manejo um paciente 2 e 4 meses portador DII. Relato caso: Paciente apresentava sangramentos, dificuldade ganho peso, úlceras perianais, internações frequentes, atraso marcos desenvolvimento anemia. Iniciou-se investigação...
Human adipose-derived stem cells (ASCs), differentiated in vitro towards Schwann-like phenotype (hdASCs), have been suggested as an alternative source of Schwann (SCs). However, although they seem a good alternative, their differentiation is unstable, losing SC-like following growth factor withdrawal. Rat and human SCs rat dASCs characterized for acetylcholine M2 muscarinic receptor subtype that plays strategic role the regulation differentiation. Here, we evaluated activation controlling...
Synaptic elements from neuromuscular junctions (NMJs) undergo massive morphological and functional changes upon nerve injury. While of NMJ-associated glia in response to injury has been investigated, their properties remain elusive. Perisynaptic Schwann cells (PSCs), glial at the NMJ, are essential for NMJ maintenance repair, involved synaptic efficacy plasticity. Importantly, these functions regulated by PSCs ability detect transmission through, notably, muscarinic (mAChRs) purinergic...
Glioblastoma (GBM) is the most aggressive human brain tumor. The high growth potential and decreased susceptibility to apoptosis of glioma cells mainly dependent on genetic amplifications or mutations oncogenic pro-apoptotic genes, respectively. We have previously shown that activation M2 acetylcholine muscarinic receptors inhibited cell proliferation induced in two GBM lines cancer stem cells. aim this study was delve into molecular mechanisms underlying M2-mediated arrest. Exploiting U87MG...
Abstract Regenerative capability of the peripheral nervous system after injury is enhanced by Schwann cells (SCs) producing several growth factors. The clinical use SCs in nerve regeneration strategies hindered necessity removing a healthy to obtain therapeutic cells. Adipose-derived stem (ASCs) can be chemically differentiated towards SC-like phenotype (dASCs), and represent promising alternative SCs. Their physiology further modulated pharmacologically targeting receptors for...
Mesenchymal stem cells (MSCs), also known as stromal mesenchymal cells, are multipotent which can be found in many tissues and organs bone marrow, adipose tissue other tissues. In particular MSCs derived from Adipose (ADSCs) the most frequently used regenerative medicine because they easy to source, rapidly expandable culture excellent differentiation potential into adipocytes, chondrocytes, cell types. Acetylcholine (ACh), important neurotransmitter Central nervous system (CNS) peripheral...
Abstract Schwann cells (SCs) play a central role in peripheral nervous system physiology and the response to axon injury. The ability of SCs proliferate, secrete growth factors, modulate immune response, migrate re-myelinate regenerating axons has been largely documented. However, there are several restrictions hindering their clinical application, such as difficulty collection slow vitro expansion. Adipose-derived stem (ASCs) present good properties for nerve regenerative medicine. When...
Schwann cells (SCs) express cholinergic receptors, suggesting a role of signaling in the control SC proliferation, differentiation and/or myelination. Our previous studies largely demonstrated that pharmacological activation M2 muscarinic receptor subtype caused an inhibition cell proliferation and promoted expression pro-myelinating genes. In order to elucidate molecular activated downstream activation, present study we investigated signal transduction pathways by orthosteric agonist...
The cross-talk between axon and glial cells during development in adulthood is mediated by several molecules. Among them are neurotransmitters their receptors, which involved the control of myelinating non-myelinating cell physiology. Our previous studies largely demonstrate functional expression cholinergic muscarinic receptors Schwann cells. In particular, M2 receptor subtype, most abundant expressed cells, inhibits proliferation downregulating proteins immature phenotype triggers...
Abstract Cannabinoids are frequently used in the treatment of neuropathic pain related to nerve injury. However, despite evidence for their roles regulation axonal guidance and synapse formation during development central nervous system (CNS), possible involvement response peripheral injury remains poorly defined knowledge its role is mostly sensory system. Following injury, contemporary repair, massive morphological functional changes reshape synaptic elements at neuromuscular junctions...
Functional characterization of muscarinic cholinergic receptors in myelinating glial cells has been well described both central and peripheral nervous system. Rat Schwann (SCs) express different receptor subtypes with the prevalence M2 subtype. The selective stimulation this subtype inhibits SC proliferation, improving their differentiation towards phenotype. In work, we describe for first time that human SCs are cholinoceptive as they several and, rat SCs, is one most abundant. Human...
Due to the microenvironment created by Schwann cell (SC) activity, peripheral nerve fibers are able regenerate. Inflammation is first response damage and removal of cellular myelin debris essential in preventing persistence local inflammation that may negatively affect regeneration. Acetylcholine (ACh) one neurotransmitters involved modulation through activity its receptors, belonging both muscarinic nicotinic classes. In this report, we evaluated expression α7 acetylcholine receptors...
Adipose-derived stem cells (ASCs) are an attractive source for regenerative medicine as they can be easily isolated, rapidly expandable in culture and show excellent vitro differentiation potential. Acetylcholine (ACh), one of the main neurotransmitters central peripheral nervous systems, plays key roles control several physiological processes also non-neural tissues. As demonstrated our previous studies, ACh contribute to rat ASCs physiology, negatively modulating proliferation migration...