A5013737213- Cystic Fibrosis Research Advances
- Advanced biosensing and bioanalysis techniques
- Sirtuins and Resveratrol in Medicine
- Metabolism and Genetic Disorders
- Graphene and Nanomaterials Applications
- Lysosomal Storage Disorders Research
- RNA Research and Splicing
- Sphingolipid Metabolism and Signaling
- Amyotrophic Lateral Sclerosis Research
- Neonatal Respiratory Health Research
- Cellular transport and secretion
- Respiratory viral infections research
- Neurogenetic and Muscular Disorders Research
- Cellular Mechanics and Interactions
- Hereditary Neurological Disorders
- Nuclear Structure and Function
- Tracheal and airway disorders
- Tuberculosis Research and Epidemiology
- Machine Learning in Bioinformatics
- Adenosine and Purinergic Signaling
- Marine Biology and Environmental Chemistry
- RNA regulation and disease
- Innovative Microfluidic and Catalytic Techniques Innovation
- Calcium signaling and nucleotide metabolism
- Bacterial biofilms and quorum sensing
Italian Institute of Technology
2020-2024
University of Genoa
2015-2023
In recent years, a number of drugs have been approved for the treatment cystic fibrosis (CF). Among them, newly released Trikafta, combination 3 (VX-661/VX-445/VX-770), holds great promise to radically improve quality life large portion patients with CF carrying 1 copy F508del, most frequent transmembrane conductance regulator (CFTR) mutation. Currently available disease-modifying work by rescuing function mutated CFTR anion channel. Recent research has shown that membrane lipids, and cell...
Malva sylvestris (MS) is a medicinal herb known worldwide for its beneficial effects due to the several active molecules present in leaves and flowers. These compounds have shown antioxidant anti-inflammatory properties thus can be helpful treatments of burns chronic wounds, characterized mainly by high levels free radicals impairments inflammatory response. In this work, we propose bilayer films as wound dressings, based on poly(vinylpyrrolidone) (PVP) sodium alginate loaded with M....
β-glucocerebrosidase is a lysosomal hydrolase involved in the catabolism of sphingolipid glucosylceramide. Biallelic loss function mutations this enzyme are responsible for onset Gaucher disease, while monoallelic represent first genetic risk factor Parkinson's disease. Despite evidence, molecular mechanism linking impairment activity with neurodegeneration still unknown. In frame, we developed two vitro neuronal models deficiency, represented by mouse cerebellar granule neurons and...
Background: Cystic Fibrosis (CF) is a genetic disorder affecting around 1 in every 3000 newborns. In the most common mutation, F508del, defective anion channel, CFTR, prevented from reaching plasma membrane (PM) by quality check control of cell. Little known about how CFTR pharmacological rescue impacts cell proteome. Methods: We used high-resolution mass spectrometry, differential ultracentrifugation, machine learning and bioinformatics to investigate both changes expression localization...
ABSTRACT Stress Granules (SG) formation is a cellular protection mechanism, constituting storage for untranslated mRNAs and RNA-binding proteins (RBPs); however, these condensates can turn into pathological aggregates, related to the onset of neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS). This transition towards cytotoxic inclusions triggered by ALS-causative mutations in RBP FUS, which lead its cytoplasmic mis-localization accumulation SG. Here, we describe SG...
Cystic fibrosis (CF) is the autosomal recessive disorder most recurrent in Caucasian populations. Different mutations involving cystic transmembrane regulator protein (CFTR) gene, which encodes CFTR channel, are involved CF. A number of life-prolonging therapies have been conceived and deeply investigated to combat this disease. Among them, administration so-called modulators, such as correctors potentiators, led quite beneficial effects. Recently, based on QSAR (quantitative structure...
The formation of the biomolecular corona represents a crucial factor in controlling biological interactions and trafficking nanomaterials. In this context, availability key epitopes exposed on surface corona, able to engage machinery, is important define fate material. While full composition can be investigated by conventional bottom-up proteomics, assessment spatial orientation proteins high-throughput fashion still challenging. work, we show that labeling with isobaric tags their native...
Few-layered graphene (FLG) treatment increases the functional response of nociceptive subpopulation sensory neurons dorsal root ganglia (DRG) in to irritants specific for chemoreceptors TRPV1 and TRPA1.
Abstract Stress granules (SG) are part of a cellular protection mechanism where untranslated messenger RNAs and RNA-binding proteins stored upon conditions stress. Compositional variations due to qualitative or quantitative protein changes can disrupt their functionality alter structure. This is the case different forms amyotrophic lateral sclerosis (ALS) causative link has been proposed between cytoplasmic de-localization mutant proteins, such as FUS (Fused in Sarcoma), formation cytotoxic...
Cystic fibrosis (CF) is the autosomal recessive disorder most recurrent in Caucasian populations. To combat this disease, many life-prolonging therapies are required and deeply investigated, including development of so-called cystic transmembrane conductance regulator (CFTR) modulators, such as correctors potentiators. Combination therapy with two series drugs led to approval several multi-drug effective treatments, Orkambi, recent promising evaluation triple-combination...
Porous transition metal oxides are widely studied as biocompatible materials for the development of prosthetic implants. Resurfacing oxide to improve antibacterial properties material is still an open issue, infections remain a major cause implant failure. We investigated functionalization porous titanium obtained by anodic oxidation with amino acids (Leucine) first step couple antimicrobial peptides surface. adopted two-step molecular deposition process follows: self-assembly...
We have recently demonstrated that bioactive molecules, extracted by high pressure and temperature from olive pomace, counteract calcium-induced cell damage to different lines. Here, our aim was study the effect of same extract on murine cortical neurons, since preservation intracellular Ca2+-homeostasis is essential for neuronal function survival. Accordingly, we treated neurons with stimuli in order evoke cytotoxic glutamatergic activation. In these conditions, high-pressure pomace...
1. Abstract Findings accumulated over time show that neurophysiological, neuropathological, and molecular alterations are present in CMT1A support the dysmyelinating rather than demyelinating nature of this neuropathy. Moreover, uniform slowing nerve conduction velocity is already manifest children does not improve throughout their life. This evidence our previous studies displaying aberrant myelin composition structure adult rats prompt us to hypothesize a axon developmental defect...
Findings accumulated over time show that neurophysiological, neuropathological, and molecular alterations are present in CMT1A support the dysmyelinating rather than demyelinating nature of this neuropathy. Moreover, uniform slowing nerve conduction velocity is already manifest children does not improve throughout their life. This evidence our previous studies displaying aberrant myelin composition structure adult rats prompt us to hypothesize a axon developmental defect peripheral nervous...
Background: Adenosine triphosphate (ATP) is the energy currency of body; it takes part in various and indispensable metabolic processes for maintenance cell homeostasis, degrading to its hydrolysis product, adenosine diphosphate (ADP). Efficient ways restore ATP are therefore necessary cells. When lacks due ischemic conditions or high demand, phosphocreatine gives phosphate group ADP that converts ATP, a reaction catalyzed by enzyme creatine kinase. For this reason, utilized as...
Background: Cystic fibrosis (CF) is the autosomal recessive disorder most common in Caucasian populations. It caused by mutations cystic transmembrane regulator protein (CFTR). CFTR predominantly expressed at apical plasma membranes of epithelial cells lining several organs, and functions as a cAMP-regulated chloride/bicarbonate channel. To address underlying causes fibrosis, two biomolecular activities are required, namely correctors to increase levels cell surface, potentiators allow...