A5020778326- Calcium signaling and nucleotide metabolism
- Advanced biosensing and bioanalysis techniques
- Cystic Fibrosis Research Advances
- T-cell and B-cell Immunology
- Adenosine and Purinergic Signaling
- Sirtuins and Resveratrol in Medicine
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- DNA and Nucleic Acid Chemistry
- Muscle metabolism and nutrition
- Biochemical and Molecular Research
- Lymphoma Diagnosis and Treatment
- Metabolism and Genetic Disorders
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Neonatal Health and Biochemistry
- Neuroscience and Neuropharmacology Research
- PARP inhibition in cancer therapy
- Prion Diseases and Protein Misfolding
- Systemic Lupus Erythematosus Research
- Sulfur Compounds in Biology
- Nitric Oxide and Endothelin Effects
- HIV/AIDS drug development and treatment
University of Genoa
2016-2025
Istituto Giannina Gaslini
2002-2006
Ospedale Policlinico San Martino
2005
Istituti di Ricovero e Cura a Carattere Scientifico
2005
Foundation for Biomedical Research
2003
Tecnologie Avanzate (Italy)
2000
HLA-E-restricted T cell receptor αβ + CD8 cytolytic lymphocytes (CTLs) exist as monoclonal expansions in the peripheral blood of some individuals. Here, we show that they recognize, with high avidity, peptides derived from UL40 protein different human cytomegalovirus (CMV) strains. Recognition results induction cytotoxicity, IFN-γ production and proliferation. Autologous cells pulsed CMV-derived become susceptible to lysis by CTLs induce their The avidity for may explain how these are...
Abscisic acid (ABA) is a phytohormone involved in fundamental physiological processes of higher plants, such as response to abiotic stress (temperature, light, drought), regulation seed dormancy and germination, control stomatal closure. Here, we provide evidence that ABA stimulates several functional activities [phagocytosis, reactive oxygen species nitric oxide (NO) production, chemotaxis] human granulocytes through signaling pathway sequentially involving pertussis toxin (PTX)-sensitive G...
Abstract Human mesenchymal stem cells (MSC) are immunosuppressive and poorly immunogenic but may act as antigen-presenting (APC) for CD4+ T-cell responses; here we have investigated their ability to serve APC in vitro CD8+ responses. MSC pulsed with peptides from viral antigens evoked interferon (IFN)-γ Granzyme B secretion specific cytotoxic T lymphocytes (CTL) were lysed, although low efficiency. transfected tumor mRNA or infected a vector carrying the Hepatitis C virus NS3Ag gene induced...
SIRT6 is an NAD(+)-dependent deacetylase with a role in the transcriptional control of metabolism and aging but also genome stability inflammation. Broad therapeutic applications are foreseen for inhibitors, including uses diabetes, immune-mediated disorders, cancer. Here we report on identification first selective inhibitors by silico screening. The most promising leads show micromolar IC50s, have significant selectivity versus SIRT1 SIRT2, active cells, as shown increased acetylation at...
Abscisic acid (ABA) is a plant hormone regulating fundamental physiological functions in plants, such as response to abiotic stress. Recently, ABA was shown be produced and released by human granulocytes, insulin-producing rat insulinoma cells, murine pancreatic β cells. autocrinally stimulates the functional activities specific for each cell type through receptor-operated signal transduction pathway, sequentially involving pertussis toxin-sensitive receptor/G-protein complex, cAMP,...
Haemophilus influenzae is a major pathogen of the respiratory tract in humans that has developed capability to exploit host NAD(P) for its nicotinamide dinucleotide requirement. This strategy organized around periplasmic enzyme termed NadN (NAD nucleotidase), which plays central role by degrading NAD into adenosine and NR (nicotinamide riboside), latter being subsequently internalized specific permease. We performed biochemical structural investigation on H. determined Zn2+-dependent...
Recently, the bioactive properties of marine collagen and hydrolysates have been demonstrated. Although there is some literature assessing general chemical features biocompatibility extracts from sponges, no data are available on biological effects sponge for biomedical and/or cosmetic purposes. Here, we studied in vitro toxicity, antioxidant, wound-healing, photoprotective four HPLC-purified fractions trypsin-digested extracts—marine (MCHs)—from C. reniformis. The results showed that MCHs...
Sirtuin 6 (SIRT6) is a sirtuin family member involved in wide range of physiologic and disease processes, including cancer glucose homeostasis. Based on the roles played by SIRT6 different organs, its ability to repress expression transporters glycolytic enzymes, inhibiting has been proposed as an approach for treating type 2 diabetes mellitus (T2DM). However, so far, lack small-molecule Sirt6 inhibitors hampered conduct vivo studies assess viability this strategy. We took advantage recently...
Previous studies showed that a subset of CD8 + cytolytic T lymphocytes expressing HLA class I-specific natural killer inhibitory receptors (iNKR) is characterized by the ability to recognize HLA-E and mediate cell receptor-dependent killing different NK cell-susceptible tumor target cells. In this study, we show can also undergo extensive proliferation in mixed lymphocyte cultures response allogeneic Analysis their activity revealed broad specificity against panel phytohemagglutinin-induced...
Summary Background Chronic idiopathic urticaria is a common skin disorder characterized by recurrent, transitory, itchy weals for more than 6 weeks. An autoimmune origin has been suggested based on the findings of auto‐antibodies (Abs) directed against either α subunit high‐affinity IgE receptor or molecule in nearly half patients. Objective To identify other autoantigen targets patients with chronic urticaria. Methods We used pooled IgG derived from 133 to screen random peptide library...
Abscisic acid (ABA) is a phytohormone recently identified as new endogenous pro-inflammatory hormone in human granulocytes. Here we report the functional activation of monocytes and vascular smooth muscle cells by ABA. Incubation with ABA evokes an intracellular Ca2+ rise through second messenger cyclic ADP-ribose, leading to NF-kappaB consequent increase cyclooxygenase-2 expression prostaglandin E2 production enhanced release MCP-1 (monocyte chemoattractant protein-1) metalloprotease-9, all...
Sirtuins (SIRTs) are classified as class III histone deacetylases (HDACs), a family of enzymes that catalyze the removal acetyl groups from ε-N-acetyl lysine residues proteins, thus counteracting activity performed by acetyltransferares (HATs). Based on their involvement in different biological pathways, ranging transcription to metabolism and genome stability, SIRT dysregulation was investigated many diseases, such cancer, neurodegenerative disorders, diabetes, cardiovascular autoimmune...
The blood-brain barrier (BBB) maintains brain homeostasis but also prevents most drugs from entering the brain. No paracellular diffusion of solutes is allowed because tight junctions that are made impermeable by expression claudin5 (CLDN5) endothelial cells. possibility regulating BBB permeability in a transient and reversible fashion strong demand for pharmacological treatment diseases. Here, we designed tested short BBB-active peptides, derived CLDN5 extracellular domains CLDN5-binding...
Cytolytic T lymphocytes (CTL) are known to recognize antigen peptides in association with major histocompatibility complex (MHC) class I molecules expressed on target cells. However, a fraction of human CD8+ CTL has been shown lyse certain natural killer (NK)-susceptible cells via still undefined mechanism(s). These cells, hereafter referred as NK-CTL, frequently composed expressing one single TCR Vβ expansion (different different individuals), display memory phenotype and express HLA...
In peptide sequencing experiments involving a single step tandem mass acquisition, leucine and isoleucine are indistinguishable because both characterized by 113 Da difference from the other fragments in MS2 spectrum. this work, we propose new method to distinguish between these two amino acids consecutive MSn experiments, exploiting gas-phase fragmentation of that leads diagnostic 69 ion. We used assess Leu/Ile residues several synthetic peptides. The procedure was then tested on tryptic...
Abstract Abscisic acid (ABA) is a phytohormone involved in fundamental processes higher plants. Endogenous ABA biosynthesis occurs also lower Metazoa, which regulates several physiological functions by activating ADP-ribosyl cyclase (ADPRC) and causing overproduction of the Ca2+-mobilizing second messenger cyclic ADP-ribose (cADPR), thereby enhancing intracellular Ca2+ concentration ([Ca2+]i). Recently, production release have been demonstrated to take place human granulocytes, where behaves...
A large fraction of mutations causing cystic fibrosis impair the function transmembrane conductance regulator (CFTR) chloride channel by reduced activity (gating defect) and/or impaired exit from endoplasmic reticulum (trafficking defect). Such defects need to be treated with separate pharmacological compounds termed potentiators and correctors, respectively. Here, we report characterization aminoarylthiazoles (AATs) as having dual activity. Cells expressing mutant CFTR were studied...
Pyruvate kinase M2 (PKM2) acts at the crossroad of growth and metabolism pathways in cells. PKM2 regulation by factors can redirect glycolytic intermediates into key biosynthetic pathway. Here we show that IGF1 regulate glycolysis rate, stimulate Ser/Thr phosphorylation decrease cellular pyruvate activity. Upon treatment found an increase dimeric form enrichment nucleus. This effect was associated to a reduction enzymatic activity reversed using metformin, which decreases Akt...
Many different unique functions have been attributed to lactoferrin (Lf), including DNA and RNA binding, transport into the nucleus, where Lf binds specific sequences activates transcription. A pentapeptide, Gly‐Arg‐Arg‐Arg‐Arg, corresponding a region of N‐terminal portion human rich in basic amino acids, was synthesized its intracellular localization investigated. Peptide internalization assayed using rhodaminated form same molecule. This peptide sequence is able be internalized within less...