A5039040961- Calcium signaling and nucleotide metabolism
- Sirtuins and Resveratrol in Medicine
- Adenosine and Purinergic Signaling
- PARP inhibition in cancer therapy
- Adipose Tissue and Metabolism
- Nicotinic Acetylcholine Receptors Study
- Bioactive Compounds and Antitumor Agents
- Autophagy in Disease and Therapy
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Tea Polyphenols and Effects
- Cancer therapeutics and mechanisms
- Biochemical effects in animals
- Cholinesterase and Neurodegenerative Diseases
- Lung Cancer Treatments and Mutations
- Meningioma and schwannoma management
- Chemical Reactions and Isotopes
- Myasthenia Gravis and Thymoma
- Pancreatic function and diabetes
- Olfactory and Sensory Function Studies
- Cancer, Stress, Anesthesia, and Immune Response
- Lymphoma Diagnosis and Treatment
- Lung Cancer Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Chemotherapy-induced cardiotoxicity and mitigation
Buck Institute for Research on Aging
2020-2022
University of Genoa
2007-2019
Washington University in St. Louis
2016-2019
Center for Biomedical Research of La Rioja
2013
National Cancer Research Institute
2007
Highlights•NMN suppresses age-associated body weight gain and enhances energy metabolism•NMN improves insulin sensitivity, eye function, other features with no toxicity•NMN prevents gene expression changes in a tissue-specific manner•NMN is an effective anti-aging intervention that could be translated to humansSummaryNAD+ availability decreases age certain disease conditions. Nicotinamide mononucleotide (NMN), key NAD+ intermediate, has been shown enhance biosynthesis ameliorate various...
Cytokine secretion by cancer cells contributes to cancer-induced symptoms and angiogenesis. Studies show that the sirtuin SIRT6 promotes inflammation enhancing TNF expression. Here, we aimed determine whether is involved in conferring an inflammatory phenotype define mechanisms linking inflammation. We enhances expression of pro-inflammatory cyto-/chemokines, such as IL8 TNF, cell migration pancreatic Ca(2+) responses. Via its enzymatic activity, increases intracellular levels ADP-ribose,...
SIRT6 is an NAD(+)-dependent deacetylase with a role in the transcriptional control of metabolism and aging but also genome stability inflammation. Broad therapeutic applications are foreseen for inhibitors, including uses diabetes, immune-mediated disorders, cancer. Here we report on identification first selective inhibitors by silico screening. The most promising leads show micromolar IC50s, have significant selectivity versus SIRT1 SIRT2, active cells, as shown increased acetylation at...
Abscisic acid (ABA) is a plant hormone regulating fundamental physiological functions in plants, such as response to abiotic stress. Recently, ABA was shown be produced and released by human granulocytes, insulin-producing rat insulinoma cells, murine pancreatic β cells. autocrinally stimulates the functional activities specific for each cell type through receptor-operated signal transduction pathway, sequentially involving pertussis toxin-sensitive receptor/G-protein complex, cAMP,...
The plant hormone abscisic acid (ABA) is released from glucose-challenged human pancreatic β cells and stimulates insulin secretion. We investigated whether plasma ABA increased during oral intravenous glucose tolerance tests (OGTTs IVGTTs) in healthy subjects. In all subjects undergoing OGTTs (n=8), over basal values (in a range 2- to 9-fold). A positive correlation was found between the area under curve (AUC) AUC. 4 out of 6 IVGTTs, little or no increase levels observed. remaining...
Sirtuin 6 (SIRT6) is a sirtuin family member involved in wide range of physiologic and disease processes, including cancer glucose homeostasis. Based on the roles played by SIRT6 different organs, its ability to repress expression transporters glycolytic enzymes, inhibiting has been proposed as an approach for treating type 2 diabetes mellitus (T2DM). However, so far, lack small-molecule Sirt6 inhibitors hampered conduct vivo studies assess viability this strategy. We took advantage recently...
Nicotinamide phosphoribosyltransferase (NAMPT) is the rate-limiting enzyme in NAD+ salvage pathway from nicotinamide. By controlling biosynthesis of NAD+, NAMPT regulates activity NAD+-converting enzymes, such as CD38, poly-ADP-ribose polymerases, and sirtuins (SIRTs). SIRT6 involved regulation a wide number metabolic processes. In this study, we investigated ability to regulate intracellular NAD(P)(H) levels. BxPC-3 cells MCF-7 were engineered overexpress catalytically active or inactive...
Abstract Nicotinic acetylcholine receptors (nAChR) are expressed on normal bronchial epithelial and nonsmall cell lung cancer (NSCLC) cells involved in growth regulation. Nicotine induced proliferation. The purpose of this study was to determine if interruption autocrine nicotinic cholinergic signaling might inhibit A549 NSCLC growth. For α‐Cobratoxin (α‐CbT), a high affinity α7‐nAChR antagonist studied. Cell decrease evaluated by Clonogenic MTT assays. Evidence apoptosis identified staining...
UV-B is an abiotic environmental stress in both plants and animals. Abscisic acid (ABA) a phytohormone regulating fundamental physiological functions plants, including response to stress. We previously demonstrated that ABA endogenous hormone also animal cells. Here, we investigated whether autocrine regulates the of human granulocytes keratinocytes, cells involved UV-triggered skin inflammation. The intracellular concentration increased UV-B-exposed keratinocytes was released into...
Peritonitis and subsequent sepsis lead to high morbidity mortality in response uncontrolled systemic inflammation primarily mediated by macrophages. Nicotinamide adenine dinucleotide (NAD+) is an important regulator of oxidative stress immunoinflammatory responses. However, the effects NAD+ replenishment during inflammatory activation are still poorly defined. Hence, we investigated whether administration β-nicotinamide mononucleotide (β-NMN), a natural biosynthetic precursor NAD+, could...
The hormone abscisic acid (ABA) is a small molecule involved in pivotal physiological functions higher plants. Recently, ABA has been also identified as an endogenous mammals, regulating different cell including inflammatory processes, stem expansion, insulin release, and glucose uptake. Aptamers are short, single-stranded (ss) oligonucleotidesable to recognize target molecules with high affinity. size of the represented challenge for aptamer development aim this study was develop specific...
β-nicotinamide mononucleotide (NMN) is a natural molecule intermediate in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Preclinical evidences point to beneficial effect NMN administration on several age-related conditions. The present work aimed at studying mutagenicity, and genotoxicity, acute oral toxicity subchronic high purity synthetic form (NMN-C®) following OECD guidelines. In experimental conditions tested, NMN-C® was not mutagenic or genotoxic. Acute assay revealed...
Doxorubicin (Doxo) is a widely used antineoplastic drug with limited clinical application due to its deleterious dose-related side effects. We investigated whether nicotinamide mononucleotide (NMN) could protect against Doxo-induced cardiotoxicity and physical dysfunction in vivo. To assess the short- long-term toxicity, two Doxo regimens were tested, acute chronic. In study, C57BL6/J (B6) mice injected intraperitoneally (i.p.) once (20 mg/kg) NMN (180 mg/kg/day, i.p.) was administered daily...