A5047823589- Calcium signaling and nucleotide metabolism
- Muscle metabolism and nutrition
- Adenosine and Purinergic Signaling
- Bacteriophages and microbial interactions
- Biochemical and Molecular Research
- Sirtuins and Resveratrol in Medicine
- Metabolism and Genetic Disorders
- RNA Interference and Gene Delivery
- Phytochemicals and Antioxidant Activities
- Advanced biosensing and bioanalysis techniques
- Cystic Fibrosis Research Advances
- PARP inhibition in cancer therapy
- Microplastics and Plastic Pollution
- Neuroscience and Neuropharmacology Research
- Cytomegalovirus and herpesvirus research
- Cultural Heritage Materials Analysis
- Cancer, Hypoxia, and Metabolism
- Marine Sponges and Natural Products
- Immunodeficiency and Autoimmune Disorders
- Conservation Techniques and Studies
- Essential Oils and Antimicrobial Activity
- Ion Channels and Receptors
- Nanoparticles: synthesis and applications
- Adipose Tissue and Metabolism
- Seaweed-derived Bioactive Compounds
University of Genoa
2016-2025
Center for Biomedical Research of La Rioja
2013
Foundation for Biomedical Research
2008
<h3>Objectives</h3> To analyse the prevalence of <i>CECR1</i> mutations in patients diagnosed with early onset livedo reticularis and/or haemorrhagic/ischaemic strokes context inflammation or polyarteritis nodosa (PAN). Forty-eight from 43 families were included study. <h3>Methods</h3> Direct sequencing was performed by Sanger analysis. Adenosine deaminase 2 (ADA2) enzymatic activity analysed monocyte isolated and healthy controls incubated adenosine without an ADA1 inhibitor....
Abscisic acid (ABA) is a phytohormone involved in fundamental physiological processes of higher plants, such as response to abiotic stress (temperature, light, drought), regulation seed dormancy and germination, control stomatal closure. Here, we provide evidence that ABA stimulates several functional activities [phagocytosis, reactive oxygen species nitric oxide (NO) production, chemotaxis] human granulocytes through signaling pathway sequentially involving pertussis toxin (PTX)-sensitive G...
Abstract Human mesenchymal stem cells (MSC) are immunosuppressive and poorly immunogenic but may act as antigen-presenting (APC) for CD4+ T-cell responses; here we have investigated their ability to serve APC in vitro CD8+ responses. MSC pulsed with peptides from viral antigens evoked interferon (IFN)-γ Granzyme B secretion specific cytotoxic T lymphocytes (CTL) were lysed, although low efficiency. transfected tumor mRNA or infected a vector carrying the Hepatitis C virus NS3Ag gene induced...
The plant hormone abscisic acid (ABA) is released from glucose-challenged human pancreatic β cells and stimulates insulin secretion. We investigated whether plasma ABA increased during oral intravenous glucose tolerance tests (OGTTs IVGTTs) in healthy subjects. In all subjects undergoing OGTTs (n=8), over basal values (in a range 2- to 9-fold). A positive correlation was found between the area under curve (AUC) AUC. 4 out of 6 IVGTTs, little or no increase levels observed. remaining...
Immunoglobulin (Ig) isotype diversification by class switch recombination (CSR) is an essential process for mounting a protective humoral immune response.Ig CSR deficiencies in humans can result from intrinsic B cell defect; however, most of these are still molecularly undefined and diagnosed as common variable immunodeficiency (CVID).Here, we show that extracellular adenosine critically contributes to human naive IgM memory cells.In cells, coordinate stimulation receptor toll-like receptors...
Aberrant histone deacetylase (HDAC) activity is frequent in human leukemias. However, while classical, NAD+-independent HDACs are an established therapeutic target, the relevance of NAD+-dependent (sirtuins) leukemia treatment remains unclear. Here, we assessed antileukemic sirtuin inhibitors and NAD+-lowering drug FK866, alone combination with traditional HDAC inhibitors. Primary cells, cell lines, healthy leukocytes hematopoietic progenitors were treated (sirtinol, cambinol, EX527) or...
Recently, the bioactive properties of marine collagen and hydrolysates have been demonstrated. Although there is some literature assessing general chemical features biocompatibility extracts from sponges, no data are available on biological effects sponge for biomedical and/or cosmetic purposes. Here, we studied in vitro toxicity, antioxidant, wound-healing, photoprotective four HPLC-purified fractions trypsin-digested extracts—marine (MCHs)—from C. reniformis. The results showed that MCHs...
In order to assess the impact of nanoplastics on marine species, polystyrene nanoparticles (PS NPs) have been largely used as model particles. Here we studied effects 50 nm amino-modified PS-NH2 Mediterranean sea urchin Paracentrotus lividus immune system cells (coelomocytes) in presence celomic fluid (CF) and at different NP concentrations (1, 5, 10, 25 μg mL−1) experimental conditions (absence or EDTA). acquired a protein corona once incubated with CF, dominated by toposome precursor...
2-Cis,4-trans-abscisic acid (ABA) is a plant hormone that present also in animals. Several lines of evidence suggest ABA contributes to the regulation glycemia mammals: nanomolar stimulates insulin release from β-pancreatic cells and glucose transporter-4-mediated uptake by myoblasts adipocytes vitro; plasma increases normal human subjects, but not diabetic patients, after load for an oral tolerance test (OGTT). The presence fruits prompted exploration bioavailability dietary effect ABA-rich...
Abscisic acid (ABA) is a phytohormone regulating important functions in higher plants, notably responses to abiotic stress. Recently, chemical or physical stimulation of human granulocytes was shown induce production and release endogenous ABA, which activates specific cell functions. Here we provide evidence that ABA stimulates several functional activities the murine microglial line N9 (NO tumor necrosis factor-alpha production, migration) through second messenger cyclic ADP-ribose an...
Introduction During thermogenesis, adipose tissue (AT) becomes more active and enhances oxidative metabolism. The promotion of this process in white AT (WAT) is called “browning” and, together with the brown (BAT) activation, considered as a promising approach to counteract obesity metabolic diseases. Transient receptor potential cation channel, subfamily M, member 2 (TRPM2), an ion channel that allows extracellular Ca 2+ influx into cytosol, gated by adenosine diphosphate ribose (ADPR),...
Abstract Abscisic acid (ABA) is a phytohormone involved in fundamental processes higher plants. Endogenous ABA biosynthesis occurs also lower Metazoa, which regulates several physiological functions by activating ADP-ribosyl cyclase (ADPRC) and causing overproduction of the Ca2+-mobilizing second messenger cyclic ADP-ribose (cADPR), thereby enhancing intracellular Ca2+ concentration ([Ca2+]i). Recently, production release have been demonstrated to take place human granulocytes, where behaves...
In several cell types, a regulated efflux of NAD+ across Connexin 43 hemichannels (Cx43 HC) can occur, and extracellular (NAD+e) affects cell-specific functions. We studied the capability bone marrow-derived human mesenchymal stem cells (MSC) to release intracellular through Cx43 HC. efflux, quantified by sensitive enzymatic cycling assay, was significantly upregulated low Ca2+ (5–6-fold), shear stress (13-fold), inflammatory conditions (3.1- 2.5-fold in incubated with lipopolysaccharide...
Pyruvate kinase M2 (PKM2) acts at the crossroad of growth and metabolism pathways in cells. PKM2 regulation by factors can redirect glycolytic intermediates into key biosynthetic pathway. Here we show that IGF1 regulate glycolysis rate, stimulate Ser/Thr phosphorylation decrease cellular pyruvate activity. Upon treatment found an increase dimeric form enrichment nucleus. This effect was associated to a reduction enzymatic activity reversed using metformin, which decreases Akt...
Microplastics (MPs) in drinking water (DW) raise concerns about their potential impacts on health. Several substances, such as plasticizers and stabilizers, can be leached from plastic polymers following abiotic biotic denaturation processes. Furthermore, the purification treatment of DW, chlorination process, significantly increases release chemical components that are part polymer composition. Recently, several studies reported MPs adsorb environmental xenobiotics, organic molecules heavy...
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UV-B is an abiotic environmental stress in both plants and animals. Abscisic acid (ABA) a phytohormone regulating fundamental physiological functions plants, including response to stress. We previously demonstrated that ABA endogenous hormone also animal cells. Here, we investigated whether autocrine regulates the of human granulocytes keratinocytes, cells involved UV-triggered skin inflammation. The intracellular concentration increased UV-B-exposed keratinocytes was released into...
The plant hormone abscisic acid (ABA) is present and active in humans, regulating glucose homeostasis. In normal tolerant (NGT) human subjects, plasma ABA (ABAp) increases 5-fold after an oral load. aim of this study was to assess the effect load on ABAp type 2 diabetes (T2D) subjects. We chose two sub-groups patients who underwent for diagnostic purposes: i) 9 treatment-naive T2D ii) pregnant women with gestational (GDM), before 8–12 weeks childbirth. Each group compared matched NGT...
The nicotinamide phosphoribosyltransferase (NAMPT) inhibitor, APO866, has been previously shown to have antileukemic activity in preclinical models, but its cytotoxicity primary leukemia cells is frequently limited. success of current treatments reduced by the occurrence multidrug resistance, which, turn, mediated membrane transport proteins, such as P-glycoprotein-1 (Pgp). Here, we evaluated effects APO866 combination with Pgp inhibitors and studied mechanisms underlying interaction between...