A5078236005- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Osteoarthritis Treatment and Mechanisms
- Periodontal Regeneration and Treatments
- 3D Printing in Biomedical Research
- Orthopaedic implants and arthroplasty
- Electrospun Nanofibers in Biomedical Applications
- Cell Adhesion Molecules Research
- Dental Implant Techniques and Outcomes
- Graphene and Nanomaterials Applications
- Extracellular vesicles in disease
- Proteoglycans and glycosaminoglycans research
- Silk-based biomaterials and applications
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Bone fractures and treatments
- Liver physiology and pathology
- Monoclonal and Polyclonal Antibodies Research
- Bone Metabolism and Diseases
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Dental materials and restorations
- Glycosylation and Glycoproteins Research
- biodegradable polymer synthesis and properties
- Connective tissue disorders research
Ospedale Policlinico San Martino
2016-2024
University of Genoa
2015-2024
University of Turin
2024
Tecnologie Avanzate (Italy)
2002-2018
Istituto Nazionale di Fisica Nucleare, Sezione di Genova
2014-2016
Stem Cell Institute
2016
Technology Centre Prague
2016
Alleanza Contro il Cancro
2001-2011
University College London
2009
Royal National Orthopaedic Hospital
2009
Bone marrow stromal cells can give rise to several mesenchymal lineages. The existence of a common stem/progenitor cell, the stem has been proposed, but which developmental stages follow this multipotent progenitor is not known. Based on experimental evidence, model cell differentiation proposed in individual lineages branch directly from same progenitor. We have verified by using clonal cultures bone derived fibroblasts. analyzed ability 185 non-immortalized human clones differentiate into...
The ability of marrow-derived osteoprogenitor cells to promote repair critical-size tibial gaps upon autologous transplantation on a hydroxyapatite ceramic (HAC) carrier was tested in sheep model. Conditions for vitro expansion bone marrow stromal (BMSC) were established and the osteogenic potential expanded validated. Ectopic implantation BMSC immunocompromised mice led extensive formation. When used sheep, cell-loaded implants (n = 2) conducted far more formation than did cell-free HAC...
Extensive bone loss is still a major problem in orthopedics. A number of different therapeutic approaches have been developed and proposed, but so far none proven to be fully satisfactory. We used new tissue engineering approach treat four patients with large diaphysis defects poor alternatives. To obtain implantable three-dimensional (3D) living constructs, cells isolated from the patients' marrow stroma were expanded culture seeded onto porous hydroxyapatite (HA) ceramic scaffolds designed...
Abstract We have investigated the effects of different growth factors on proliferation and osteogenic potential primary cultures human bone marrow stromal cells (BMSC). Fibroblast factor (FGF)-2 was most effective in promoting these vitro. The size colonies formed clonal conditions approximately 2.5 times larger presence FGF-2. Also morphology BMSC affected: cultured 10% FCS alone became flattened, whereas FGF-2 expanded maintained a fibroblast-like elongated phenotype. Levels alkaline...
Bone marrow stromal cells (BMSCs) can be easily isolated from adult and contain a population of pluripotent progenitors that give rise to different mesenchymal lineages both in vitro vivo. These properties make BMSCs an attractive target for cell-based therapeutic strategies variety disorders. However, because their low frequency vivo, obtain sufficient number tissue engineering step extensive expansion is required, which could significantly alter BMSC properties. Therefore, effective use...
The development of a new family implantable bioinspired materials is focal point bone tissue engineering. Implant surfaces that better mimic the natural extracellular matrix, naturally nano-composite tissue, can stimulate stem cell differentiation towards osteogenic lineages in absence specific chemical treatments. Herein we describe bioactive composite nanofibrous scaffold, composed poly-caprolactone (PCL) and nano-sized hydroxyapatite (HA) or beta-tricalcium phosphate (TCP), which was able...
Three-dimensional (3D) culture systems are critical to investigate cell physiology and engineer tissue grafts. In this study, we describe a simple yet innovative bioreactor-based approach seed, expand, differentiate bone marrow stromal cells (BMSCs) directly in 3D environment, bypassing the conventional process of monolayer (two-dimensional [2D]) expansion. The system, based on perfusion marrow-nucleated through porous scaffolds, supported formation stromal-like tissues, where BMSCs could be...
Abstract The objective of this article was to investigate the safety and regenerative potential a newly developed biomimetic scaffold when applied osteochondral defects in an animal model. A new multilayer gradient nano‐composite obtained by nucleating collagen fibrils with hydroxyapatite nanoparticles. In femoral condyles 12 sheep, 24 lesions were created. Animals randomized into three treatment groups: alone, colonized vitro autologous chondrocytes empty defects. Six months after surgery,...
Hyperplasia of the parathyroid glands is a central feature familial multiple endocrine neoplasia type 1. We used cultured bovine cells to test for mitogenic activity in plasma from patients with this disorder. Normal stimulated [3H]thymidine incorporation, on average, same extent as it was plasma-free control culture. This contrasted results tests 1, which increased 2400 percent over value (P<0.001). Plasma these also proliferation culture, whereas normal subjects inhibited it. Parathyroid 1...