A5017105000- Angiogenesis and VEGF in Cancer
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Coronary Interventions and Diagnostics
- Periodontal Regeneration and Treatments
- Axon Guidance and Neuronal Signaling
- Osteoarthritis Treatment and Mechanisms
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- 3D Printing in Biomedical Research
- Wound Healing and Treatments
- Virus-based gene therapy research
- Peripheral Artery Disease Management
- Venous Thromboembolism Diagnosis and Management
- Cancer, Hypoxia, and Metabolism
- Bone Tissue Engineering Materials
- Congenital heart defects research
- RNA Interference and Gene Delivery
- Lymphatic System and Diseases
- Management of metastatic bone disease
- Blood properties and coagulation
- Orthopaedic implants and arthroplasty
- Proteoglycans and glycosaminoglycans research
- Cardiac and Coronary Surgery Techniques
University Hospital of Basel
2016-2025
University of Basel
2016-2025
Politecnico di Milano
2015
Baxter (United States)
2003-2012
Stanford University
2001-2012
Luzerner Kantonsspital
2006
Alleanza Contro il Cancro
2000-2003
Tecnologie Avanzate (Italy)
2000-2003
University of Genoa
2000-2003
Use of long-term constitutive expression VEGF for therapeutic angiogenesis may be limited by the growth abnormal blood vessels and hemangiomas. We investigated relationship between dosage morphology function newly formed implanting retrovirally transduced myoblasts that constitutively express VEGF164 into muscles adult mice. Reducing decreasing total number implanted did not prevent vascular abnormalities. However, when clonal populations homogeneously expressing different levels were...
Endothelial cell (EC)-derived signals contribute to organ regeneration, but angiocrine metabolic communication is not described. We found that EC-specific loss of the glycolytic regulator pfkfb3 reduced ischemic hindlimb revascularization and impaired muscle regeneration. This was caused by ability macrophages adopt a proangiogenic proregenerative M2-like phenotype. Mechanistically, lactate secretion ECs lowered levels in muscle. Addition pfkfb3-deficient restored polarization an...
Circulating tumor cells (CTCs) are shed from solid cancers in the form of single or clustered cells, and latter display an extraordinary ability to initiate metastasis. Yet, biological phenomena that trigger shedding CTC clusters a primary cancerous lesion poorly understood. Here, when dynamically labeling breast cancer along progression, we observe majority undergoing hypoxia, while CTCs largely normoxic. Strikingly, find vascular endothelial growth factor (VEGF) targeting leads shrinkage,...
Bone marrow stromal cells (BMSCs) can be easily isolated from adult and contain a population of pluripotent progenitors that give rise to different mesenchymal lineages both in vitro vivo. These properties make BMSCs an attractive target for cell-based therapeutic strategies variety disorders. However, because their low frequency vivo, obtain sufficient number tissue engineering step extensive expansion is required, which could significantly alter BMSC properties. Therefore, effective use...
Significance Inducing the growth of new blood vessels by specific factors is an attractive strategy to restore flow in ischemic tissues. Vascular endothelial factor (VEGF) master regulator angiogenesis, yet clinical trials VEGF gene delivery failed. Major challenges include need control tissue distribution dose and duration expression. Here, we developed a highly tunable fibrin-based platform precisely protein Optimized fibrin-bound ensured normal, stable, functional angiogenesis improved...
VEGF induces normal or aberrant angiogenesis depending on its dose in the microenvironment around each producing cell vivo. This transition depends balance between VEGF-induced endothelial stimulation and PDGF-BB-mediated pericyte recruitment, co-expression of PDGF-BB normalizes despite high doses. We recently found that over-expression skeletal muscle through an initial circumferential vascular enlargement followed by longitudinal splitting, rather than sprouting. Here we investigated...
Rapid vascularization of clinical-size bone grafts is an unsolved challenge in regenerative medicine. Vascular endothelial growth factor-A (VEGF) the master regulator angiogenesis. Its over-expression by genetically modified human osteoprogenitors has been previously evaluated to drive osteogenic grafts, but observed cause paradoxical loss through excessive osteoclast recruitment. However, during development angiogenesis and osteogenesis are physiologically coupled VEGF expression. Here we...
Vascular endothelial growth factor-A (VEGF) physiologically regulates both angiogenesis and osteogenesis, but its application in bone tissue engineering led to contradictory outcomes. A poorly understood aspect is how VEGF dose impacts the coordination between these two processes. Taking advantage of a unique highly tunable platform, here we dissected effects over 1,000-fold range context tissue-engineered osteogenic grafts. We found that osteo-angiogenic coupling exquisitely dependent on...
BACKGROUND Osteoporosis is a sequela of hemopoietic cell transplantation with complex multifactorial pathogenesis in which the relative role chemotherapy and irradiation not completely understood. Therefore, authors investigated toxicity chemotherapy-only conditioning regimens on bone homeostasis marrow osteoprogenitors, its dose dependency, mechanism chemotherapy-induced osteopenia. METHODS Fifty-one patients high-grade non-Hodgkin lymphoma or breast carcinoma who had been treated...
The critical role of vascular endothelial growth factor (VEGF) expression levels in developmental angiogenesis is well established. Nonetheless, the effects different local (microenvironmental) VEGF concentrations ischemia have not been studied adult organism, and delivery to patients has disappointing. Here, we demonstrate existence both lower upper threshold microenvironmental for induction therapeutic vessel ischemia. In ischemic hind limb, implantation myoblasts transduced express...
Therapeutic angiogenesis by delivery of vascular growth factors is an attractive strategy for treating debilitating occlusive diseases, yet clinical trials have thus far failed to show efficacy. As a result, limb amputation remains common outcome muscle ischemia due severe atherosclerotic disease, with overall incidence 100 per million people in the United States year. A challenge has been that angiogenic master regulator endothelial factor (VEGF) induces dysfunctional vessels, if expressed...
Article11 April 2018Open Access Transparent process EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF Elena Groppa Department of Biomedicine, University Hospital, Basel, Switzerland Surgery, Search for more papers this author Sime Brkic Andrea Uccelli Galina Wirth A. I. Virtanen Institute, Eastern Finland, Kuopio, Finland Petra Korpisalo-Pirinen Maria Filippova Boris Dasen Veronica Sacchi Manuele Giuseppe Muraro Marianna Trani Silvia Reginato Roberto Gianni-Barrera Seppo...
The advancement of in vitro engineered cardiac tissue-based patches is paramount for providing viable solutions restoring function through vivo implantation. Numerous techniques described the literature aim to provide diverse mechanical and topographical cues simultaneously, fostering enhanced maturation functionality. Among these, cellulose paper-based scaffolds have gained attention owing their inherent benefits, such as biocompatibility ease chemical physical modification. This study...