A5062457632- Mesenchymal stem cell research
- Developmental Biology and Gene Regulation
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Hematopoietic Stem Cell Transplantation
- Hippo pathway signaling and YAP/TAZ
- Periodontal Regeneration and Treatments
- Genomics and Chromatin Dynamics
- Electrospun Nanofibers in Biomedical Applications
- Wnt/β-catenin signaling in development and cancer
- Dental Implant Techniques and Outcomes
- Extracellular vesicles in disease
- Osteoarthritis Treatment and Mechanisms
- Bone and Dental Protein Studies
- Spine and Intervertebral Disc Pathology
- Graphene and Nanomaterials Applications
- Spaceflight effects on biology
- Tissue Engineering and Regenerative Medicine
- Cellular Mechanics and Interactions
- Chromosomal and Genetic Variations
- biodegradable polymer synthesis and properties
- Animal Genetics and Reproduction
- Medical Imaging and Analysis
- Musculoskeletal pain and rehabilitation
University of Basel
2018-2023
University Hospital of Basel
2018-2023
Arcadis (Switzerland)
2023
University of Zurich
2023
New York University
2023
Hanyang University
2023
Rapid vascularization of clinical-size bone grafts is an unsolved challenge in regenerative medicine. Vascular endothelial growth factor-A (VEGF) the master regulator angiogenesis. Its over-expression by genetically modified human osteoprogenitors has been previously evaluated to drive osteogenic grafts, but observed cause paradoxical loss through excessive osteoclast recruitment. However, during development angiogenesis and osteogenesis are physiologically coupled VEGF expression. Here we...
Significance Recapitulating human blood cancers in vitro remains challenging due to the limitations of current models culture patient-derived malignant hematopoietic stem and progenitor cells entirely bone marrow microenvironments. We demonstrate that from patients with acute myeloid leukemia myeloproliferative neoplasms can be cultured for at least 3 wk fully cell-based three-dimensional osteoblastic niches engineered perfusion bioreactors, while exhibiting key features found native marrow....
Background: Devitalized bone matrix (DBM) is currently the gold standard alternative to autologous grafting in maxillofacial surgery. However, it fully relies on its osteoconductive properties and therefore requires defects with healthy surrounding. Fractionated human adipose tissue, when differentiated into hypertrophic cartilage vitro, was proven reproducibly osteogenic vivo, by recapitulating endochondral ossification (ECO). Both types of substitutes were thus compared an orthotopic,...
Hematopoietic stem and progenitor cells (HSPCs) are frequently located around the bone marrow (BM) vasculature. These so-called perivascular niches regulate HSC function both in health disease, but they have been poorly studied humans due to scarcity of models integrating complete human vascular structures. Herein, we propose stromal fraction (SVF) derived from adipose tissue as a cell source vascularize 3D osteoblastic BM engineered perfusion bioreactors. We show that SVF form...
Bioreactors enabling direct perfusion of cell suspensions or culture media through the pores 3D scaffolds have long been used in tissue engineering to improve seeding efficiency as well uniformity distribution and development. A macro-scale U-shaped bioreactor for under (U-CUP) has previously developed. In that system, geometry chamber results rather uniform flow most scaffold volume, but not peripheral regions. Here, design optimized provide a more homogenous scaffold. Then, this...
Pericytes are mural cells that play an important role in regulation of angiogenesis and endothelial function. Cadherins a superfamily adhesion molecules mediating Ca 2+ -dependent homophilic cell-cell interactions control morphogenesis tissue remodeling. To date, classical N-cadherin is the only cadherin described on pericytes. Here, we demonstrate pericytes also express T-cadherin (H-cadherin, CDH13), atypical glycosyl-phosphatidylinositol (GPI)-anchored member has previously been...
We previously described an immortalized, genetically-engineered human Mesenchymal stromal cell line to generate BMP2-enriched Chondrogenic Matrices (MB-CM), which after devitalization and storage could efficiently induce ectopic bone tissue by endochondral ossification. In order increase the efficiency of MB-CM utilization towards engineering scaled-up structures, here we hypothesized that can retain osteoinductive properties when combined with osteoconductive material. first tested...
Chromatin insulators have been proposed to play an important role in chromosome organization and local regulatory interactions. In Drosophila , one of these is known as Wari. It located immediately downstream the 3' end white transcription unit. Wari has interact with promoter region, thereby facilitating recycling RNA polymerase machinery. We tested this model by deleting insulator at endogenous locus could not detect a significant effect on eye pigmentation.
Abstract The establishment of tissue axes is fundamental during embryonic development. In the Drosophila wing, anterior/posterior (AP) and dorsal/ventral (DV) compartment boundaries provide basic coordinates around which develops. These arise as a result two lineage decisions, acquisition posterior fate by selector gene engrailed ( en) dorsal apterous ap ). While en expression domain set up embryogenesis, only starts early wing Thus, correct pattern with respect to must be tightly...
The establishment of tissue axes is fundamental during embryonic development. In the Drosophila wing, anterior/posterior (AP) and dorsal/ventral (DV) compartment boundaries provide basic coordinates around which develops. These arise as a result two lineage decisions, acquisition posterior fate by selector gene engrailed ( en) dorsal apterous ap ). While en expression domain set up embryogenesis, only starts early wing Thus, correct pattern with respect to must be tightly controlled. Here we...
The establishment of tissue axes is fundamental during embryonic development. In the Drosophila wing, anterior/posterior (AP) and dorsal/ventral (DV) compartment boundaries provide basic coordinates around which develops. These arise as a result two lineage decisions, acquisition posterior fate by selector gene engrailed ( en) dorsal apterous ap ). While en expression domain set up embryogenesis, only starts early wing Thus, correct pattern with respect to must be tightly controlled. Here we...