Abstract The noninvasive analysis of living cells grown on 3‐dimensional scaffold materials is a key point in tissue engineering. In this work we show the capability Raman spectroscopy for use as method to distinguish at different stages cell cycle and from dead cells. spectral differences between are characterized mainly by variations DNA vibrations 782, 788, 1095 cm −1 . spectrum human lung derived (A549 line) indicates breakdown both phosphodiester bonds bases. most sensitive peak...

We report the first Raman spectra of individual living and dead cells (MLE‐12 line) cultured on bioinert standard poly‐L‐lysine coated fused silica bioactive 45S5 Bioglass ® measured at 785 nm laser excitation. At this excitation wavelength no damage was induced to even after 40 minutes irradiation 115 mW power, as indicated by cell morphology observation trypan blue viability test. show that shorter lasers, 488 514 nm, cannot be used because they induce very low powers (5 mW) short times...

Abstract We investigated the use of Raman microspectroscopy to monitor molecular changes in human lung carcinoma epithelial cells (A549) when cell death was induced by a toxic chemical. treated A549 with 100 μM Triton X‐100 and carried out measurements parallel viability DNA integrity assays at time points 0, 24, 48, 72 hours. found that important biochemical taking place during death, such as degradation proteins, breakdown, formation lipid vesicles, can be detected microspectroscopy. A...

Abstract The use of polymethylmethacrylate (PMMA) cement to reinforce fragile or broken vertebral bodies (vertebroplasty) leads extensive bone stiffening. Fractures in the adjacent vertebrae may be consequence this procedure. PMMA with a reduced Young's modulus more suitable. goal study was produce and characterize stiffness adapted cements. Porous cements were produced by combining various volume fractions an aqueous sodium hyaluronate solution. Porosity, modulus, yield strength,...

It is well accepted, that there communication between osteoblasts and endothelial cells. However, the influence of cells on differentiation bone precursors into mature not yet understood. We therefore studied effect human umbilical vein (HUVEC) marrow stromal cell (BMSC) towards an osteoblastic phenotype by culturing them in two different types HUVEC-BMSC cultures (indirect contact, HUVEC-conditioned medium). Typical markers (45Ca incorporation, ALP activity, gene expression collagen 1...

Tissue engineering techniques for the regeneration of large bone defects require sufficient vascularisation applied constructs to ensure a supply oxygen and nutrients. In our previous work, prevascularised 3D scaffolds have been successfully established by coculture marrow derived stem cells (MSCs) endothelial progenitor (EPCs). We identified stabilising pericytes (PCs) as part newly formed capillary-like structures. present study, we report preliminary data on interactions between MSCs...

Blood supply is a critical issue in most tissue engineering approaches for large defect healing. As vessel ingrowth from surrounding tissues proven to be insufficient, current strategies are focusing on the neo-vascularisation process. In present study, we developed an vitro pre-vascularised construct using 3D polyurethane (PU) scaffolds, based association of human Endothelial Progenitor Cells (EPC, CD34+ and CD133+) with Mesenchymal Stem (MSC). We showed formation luminal tubular structures...

Platelet-rich plasma (PRP) has been used for different applications in human and veterinary medicine. Many studies have shown promising therapeutic effects of PRP; however, there are still many controversies regarding its composition, properties, clinical efficacy. The aim this study was to evaluate the influence platelet concentrations on rheological properties growth factor (GF) release profile PRP-gels. In addition, viability incorporated bone marrow-derived mesenchymal stem cells (MSCs)...

Vascularization is critical for 3D tissue engineered constructs. In large size implants the ingrowth of vessels often fails. The purpose this study was to identify an easily accessible, clinically relevant cell source able promote neovascularization in vivo and establish autologous culture method these cells. MSCs (mesenchymal stem cells) endothelial progenitor containing (EPCC) population were obtained from human bone marrow aspirates. expression endothelial-markers, uptake acetylated low...

(1) In vitro, bone marrow-derived stromal cells (BMSCs) demonstrate inter-donor phenotypic variability, which presents challenges for the development of regenerative therapies. Here, we investigated whether frequency putative BMSC sub-populations within freshly isolated mononuclear cell fraction marrow is phenotypically predictive in vitro derived culture. (2) Vertebral body, iliac crest, and femoral head were acquired from 33 patients (10 female 23 male, age range 14–91). identified...

Both Fourier Transform Infrared (FTIR) and Raman spectroscopy have been applied to the in vitro characterisation of biomaterials, mainly surface reactions leading formation a biologically active hydroxycarbonate apatite (HCA) layer on sample when immersed simulated body fluids (SBF). The HCA indicates degree bioactivity sample, because it leads strong bond between biomaterial living tissue. Reflection measurements using FTIR allow quick, non‐destructive detection for solid powder samples....

Pericyte recruitment is essential for the stability of newly formed vessels.It was also suggested that pericytes represent common ancestor cells giving rise to mesenchymal stem (MSCs) in adult.Here, we systematically investigated and MSCs from different human tissues terms their angiogenic multilineage differentiation potential vitro order assess suitability cell types regeneration vascularised tissues.Magnetic-activated sorting (MACS ® ) used enrich CD34-CD146+ adipose tissue (AT) bone...

Fetal bovine serum (FBS) and fibroblast growth factor (FGF)-2 are key supplements for the culture of stromal vascular fraction (SVF) cells from adipose tissue, both typical monolayer (2D) expansion streamlined generation osteogenic-vasculogenic grafts in 3D perfusion culture. The present study investigates whether factors human platelet lysate (PL) could substitute FBS FGF-2 2D models SVF lipoaspirates. were grown medium supplemented with 10% FBS+FGF-2 or 5% PL. In cultures, PL initially...

The cholecystokinin (CCK) receptor-1 (CCK1R) is a G protein-coupled receptor, which mediates important central and peripheral actions. Our aim was to progress in mapping of the CCK1R binding site by identifying residues that interact with methionine phenylalanine C-terminal moiety CCK because these are crucial for its biological activity, determine whether selective non-peptide agonist, SR-146,131, share common site. Identification putative amino acids achieved dynamics-based docking ligand...