A5046644019- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Advanced Biosensing Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- Advanced biosensing and bioanalysis techniques
- Receptor Mechanisms and Signaling
- Biochemical and Structural Characterization
- Cancer, Hypoxia, and Metabolism
- Paleontology and Evolutionary Biology
- Developmental Biology and Gene Regulation
- Cardiac Structural Anomalies and Repair
- Cell Adhesion Molecules Research
- Congenital heart defects research
- Antimicrobial Peptides and Activities
- Bone fractures and treatments
- Lipid Membrane Structure and Behavior
- Lipid metabolism and biosynthesis
- Neurobiology and Insect Physiology Research
- Neuroblastoma Research and Treatments
- Tissue Engineering and Regenerative Medicine
- Nanopore and Nanochannel Transport Studies
- Animal testing and alternatives
University of Basel
2016-2023
Novartis Institutes for BioMedical Research
2013-2019
Novartis (Switzerland)
2011-2019
Extracellular vesicles (EVs) are natural nanoparticles that mediate intercellular transfer of RNA and proteins great medical interest; serving as novel biomarkers potential therapeutic agents. However, there is little consensus on the most appropriate method to isolate high-yield high-purity EVs from various biological fluids. Here, we describe a systematic comparison between two protocols for EV purification: ultrafiltration with subsequent liquid chromatography (UF-LC) differential...
Exosomes are nanovesicles released by virtually all cells, which act as intercellular messengers transfer of protein, lipid, and RNA cargo. Their quantitative efficiency, routes cell uptake, subcellular fate within recipient cells remain elusive. We quantitatively characterize exosome saturates with dose time reaches near 100% transduction efficiency at picomolar concentrations. Highly reminiscent pathogenic bacteria viruses, exosomes recruited single vesicles to the body surfing on...
Circulating tumor cells (CTCs) are shed from solid cancers in the form of single or clustered cells, and latter display an extraordinary ability to initiate metastasis. Yet, biological phenomena that trigger shedding CTC clusters a primary cancerous lesion poorly understood. Here, when dynamically labeling breast cancer along progression, we observe majority undergoing hypoxia, while CTCs largely normoxic. Strikingly, find vascular endothelial growth factor (VEGF) targeting leads shrinkage,...
Extracellular vesicles (EV) convey biological information by transmitting macromolecules between cells and tissues are of great promise as pharmaceutical nanocarriers, therapeutic per se. Strategies for customizing the EV surface cargo being developed to enable their tracking, visualization, loading with agents decoration tissue targeting ligands. While much progress has been made in engineering EVs, an exhaustive comparative analysis most commonly exploited EV-associated proteins, well a...
Abstract Extracellular vesicles (EVs) are highly interesting for the design of next-generation therapeutics. However, their preparation methods face challenges in standardization, yield, and reproducibility. Here, we describe a efficient reproducible EV method monodisperse nano plasma membrane (nPMVs), which yields 10 to 100 times more particles per cell hour than conventional methods. nPMVs produced by homogenizing giant following blebbing apoptotic body secretion induced chemical...
Abstract Confocal microscopy is used today on a daily basis in life science labs. This “routine” technique contributes to the progress of scientific projects across many fields by revealing structural details and molecular localization, but researchers need be aware that detection efficiency emission light path performance major influence confocal image quality. By design, large portion signal discarded imaging, leading decreased signal-to-noise ratio (SNR) which turn limits resolution. A...
Adipose tissue, via sympathetic and possibly sensory neurons, communicates with the central nervous system (CNS) to mediate energy homeostasis. In contrast system, morphology, role regulation of in adipose tissue are poorly characterized. Taking advantage recent progress whole-mount three-dimensional imaging, we identified a network calcitonin gene-related protein (CGRP)-positive neurons murine white (WAT). We found that mammalian target rapamycin complex 2 (mTORC2), major component insulin...
Cardiac progenitor cell-derived extracellular vesicles (CPC-EVs) have been successfully applied via different delivery routes for treating post-myocardial infarction injury in several preclinical models. Hence, understanding the vivo fate of CPC-EVs after systemic or local, i.e. myocardial, is utmost importance further therapeutic application cardiac repair. Here, we studied tissue- and cell distribution retention intramyocardial intravenous injection mice by employing EV labeling imaging...
The increasing involvement of academic institutions and biotech companies in drug discovery calls for cost-effective methods to identify new bioactive molecules. Affinity-based on-bead screening combinatorial one-bead one-compound libraries combines a split-mix synthesis design with simple protein binding assay operating directly at the bead matrix. However, one bottleneck scale is unavailability cheap, automated, robust platform that still provides quantitative signal related amount target...
The present protocol describes optical tissue clearing of murine and zebrafish with CUBIC L or HL delipidation reagents RA refractive index matching reagent (developed by Tainaka et al., 2018), adaptations modifications. does not describe ScaleCUBIC-1 ScaleCUBIC-2 the 1st version method (Susaki, Etsuo A. 2014). covers steps solution preparation, fixation, delipidation, matching, optional nuclear staining embedding samples in agarose for image acquisition on light sheet fluorescence...
Abstract Confocal microscopy is used today on a daily basis in life science labs. This “routine” technique contributes to the progress of scientific projects across many fields by revealing structural details and molecular localization, but researchers need be aware that detection efficiency emission light path performance major influence confocal image quality. By design, large portion signal discarded imaging, leading decreased signal-to-noise ratio (SNR) which turn limits resolution. A...
Adipose tissue, via sympathetic and sensory neurons, communicates with the central nervous system (CNS) to mediate energy homeostasis. In contrast system, morphology, role regulation of in adipose tissue is poorly characterized. Taking advantage recent progress whole-mount three-dimensional imaging we identified a neuronal network calcitonin gene-related protein (CGRP)-positive neurons white (WAT). Furthermore, show that mammalian target rapamycin complex 2 (mTORC2), major component insulin...