A5087743314- Pancreatic and Hepatic Oncology Research
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Advanced Proteomics Techniques and Applications
- Parallel Computing and Optimization Techniques
- RNA modifications and cancer
- Distributed and Parallel Computing Systems
- Bone health and osteoporosis research
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- RNA Research and Splicing
- MicroRNA in disease regulation
- Pharmaceutical and Antibiotic Environmental Impacts
- RNA and protein synthesis mechanisms
- Hip and Femur Fractures
- Microbial Community Ecology and Physiology
- Virus-based gene therapy research
- Mass Spectrometry Techniques and Applications
- Cancer Research and Treatments
- Ubiquitin and proteasome pathways
- Ferroptosis and cancer prognosis
- Bone health and treatments
- Muscle Physiology and Disorders
- Radiomics and Machine Learning in Medical Imaging
- Metabolomics and Mass Spectrometry Studies
Karolinska Institutet
2016-2025
Science for Life Laboratory
2016-2025
University of Gothenburg
2014-2025
Blekinge Institute of Technology
2023
Karolinska University Hospital
2021
Sahlgrenska University Hospital
2017
Australian Catholic University
2017
Svenska Örtmedicinska Institute
2016
University of Sheffield
2008-2012
University of Tartu
2008-2011
Abstract Cells release nano-sized membrane vesicles that are involved in intercellular communication by transferring biological information between cells. It is generally accepted cells at least three types of extracellular (EVs): apoptotic bodies, microvesicles and exosomes. While a wide range putative functions have been attributed to exosomes, they assumed represent homogenous population EVs. We hypothesized the existence subpopulations exosomes with defined molecular compositions...
Extracellular vesicles (EVs) are natural nanoparticles that mediate intercellular transfer of RNA and proteins great medical interest; serving as novel biomarkers potential therapeutic agents. However, there is little consensus on the most appropriate method to isolate high-yield high-purity EVs from various biological fluids. Here, we describe a systematic comparison between two protocols for EV purification: ultrafiltration with subsequent liquid chromatography (UF-LC) differential...
Mesenchymal stem cells (MSC) are known to facilitate healing of ischemic tissue related diseases through proangiogenic secretory proteins. Recent studies further show that MSC derived exosomes function as paracrine effectors angiogenesis, however, the identity which components exosome proteome responsible for this effect remains elusive. To address we used high-resolution isoelectric focusing coupled liquid chromatography tandem mass spectrometry, an unbiased high throughput proteomics...
Cell-penetrating peptides (CPPs) are able to promote uptake of various cargos, including proteins and plasmids. Advances in recent years imply the be endocytic, where current hurdle for efficient intracellular delivery is material being retained endosomes. In this study we wanted compare ability established CPPs deliver siRNA induce gene silencing luciferase, with a novel designed penetratin analog having endosomolytic properties, using noncovalent strategy. principal, EB1 will, upon...
While small interfering RNAs (siRNAs) have been rapidly appreciated to silence genes, efficient and non-toxic vectors for primary cells systemic in vivo delivery are lacking. Several siRNA-delivery vehicles, including cell-penetrating peptides (CPPs), developed but their utility is often restricted by entrapment following endocytosis. Hence, developing CPPs that promote endosomal escape a prerequisite successful siRNA implementation. We here present novel CPP, PepFect 6 (PF6), comprising the...
Abstract In the preceding decades, molecular characterization has revolutionized breast cancer (BC) research and therapeutic approaches. Presented herein, an unbiased analysis of tumor proteomes, inclusive 9995 proteins quantified across all tumors, for first time recapitulates BC subtypes. Additionally, poor-prognosis basal-like luminal B tumors are further subdivided by immune component infiltration, suggesting current classification is incomplete. Proteome-based networks distinguish...
Quantitative proteomics by mass spectrometry is widely used in biomarker research and basic biology for investigation of phenotype level cellular events. Despite the wide application, methodology statistical analysis differentially expressed proteins has not been unified. Various methods such as t test, linear model mixed effect models are to define changes experiments. However, none these consider specific structure MS-data. Choices between methods, often originally developed other types...
The use of CPPs (cell-penetrating peptides) as delivery vectors for bioactive molecules has been an emerging field since 1994 when the first CPP, penetratin, was discovered. Since then, several CPPs, including widely used Tat (transactivator transcription) peptide, have developed and utilized to translocate a wide range compounds across plasma membrane cells both in vivo vitro. Although emerged possible future candidate drug delivery, little attention given potential toxic side effects that...
Cell-penetrating peptides (CPPs) are a growing family of that have opened new avenue in drug delivery, allowing various hydrophilic macromolecules to enter cells. In accordance with most other cationic delivery vectors, CPPs seem rely mostly on endocytosis for internalization. However, due conflicting results the exact endocytic pathways CPP uptake not yet been resolved. Here, we evaluated ability seven CPPs, different chemical properties, convey peptide nucleic acids (PNAs) inside Assays...
Cell-penetrating peptides (CPPs) have attracted increasing attention in the past decade as a result of their high potential to convey various, otherwise impermeable, bioactive agents across cellular plasma membranes. Albeit different CPPs proven potent delivery cargoes, there is generally correlation between efficacy and cytotoxicity for these peptides. Hence, it great importance find new, non-toxic with more widespread properties. We present novel CPP, M918, that efficiently translocates...
Extracellular vesicles (EVs) play a significant role in cell-cell communication numerous physiological processes and pathological conditions, offer promise as novel biomarkers therapeutic agents for genetic diseases. Many recent studies have described different molecular mechanisms that contribute to EV biogenesis release from cells. However, little is known about how external stimuli such cell culture conditions can affect the quantity content of EVs. While N2a neuroblastoma cells cultured...
Abstract Mesenchymal stromal cells (MSCs) have been investigated as a treatment for various inflammatory diseases because of their immunomodulatory and reparative properties. However, many basic questions concerning mechanisms action after systemic infusion remain unanswered. We performed detailed analysis the properties proteomic profile MSCs systemically administered to two patients with severe refractory acute respiratory distress syndrome (ARDS) on compassionate use basis attempted...
Proteogenomics enable the discovery of novel peptides (from unannotated genomic protein-coding loci) and single amino acid variant (derived from single-nucleotide polymorphisms mutations). Increasing reliability these identifications is crucial to ensure their usefulness for genome annotation potential application as neoantigens in cancer immunotherapy. We here present integrated proteogenomics analysis workflow (IPAW), which combines peptide discovery, curation, validation. IPAW includes...
Extracellular vesicles (EVs) mediate cell-to-cell communication by delivering or displaying macromolecules to their recipient cells. While certain broad-spectrum EV effects reflect protein cargo composition, others have been attributed individual EV-loaded molecules such as specific miRNAs. In this work, we investigated the contents of vesicular using small RNA sequencing cells and EVs from HEK293T, RD4, C2C12, Neuro2a C17.2. The majority content in (49-96%) corresponded rRNA-, coding- tRNA...
Malassezia commensal yeasts are associated with a number of skin disorders, such as atopic eczema/dermatitis and dandruff, they also can cause systemic infections. Here we describe the 7.67-Mbp genome sympodialis, species eczema, contrast its repertoire that globosa, well those other closely related fungi. Ninety percent predicted M. sympodialis protein coding genes were experimentally verified by mass spectrometry at level. We identified relatively limited to lipid biosynthesis, both lack...
Although metabolic reprogramming within tumor cells and microenvironment (TME) is well described in breast cancer, little known about how the interplay of immune state cancer metabolism evolves during treatment. Here, we characterize immunometabolic profiles tissue samples longitudinally collected from individuals with before, after neoadjuvant chemotherapy (NAC) using proteomics, genomics histopathology. We show that pre-, on-treatment dynamic changes state, proteins cell gene expression...
Abstract Background Directing splicing using oligonucleotides constitutes a promising therapeutic tool for variety of diseases such as β‐thalassemia, cystic fibrosis, and certain cancers. The rationale is to block aberrant splice sites, thus directing the pre‐mRNA towards desired protein product. One difficulties in this setup poor bioavailability oligonucleotides, most frequently used transfection agents are unsuitable vivo use. Here we present splice‐correcting peptide nucleic acids...
The tumor suppressor p14ARF is widely deregulated in many types of cancers and believed to function as a failsafe mechanism, inhibiting proliferation inducing apoptosis cellular response high oncogene load. We have found that 22-amino-acid-long peptide derived from the N-terminal part p14ARF, denoted ARF(1-22), which has previously been shown mimic cell-penetrating properties. This internalized same extent (CPP) TP10 dose-dependently decreases MCF-7 MDA MB 231 cells. Uptake ARF(1-22)...
Malassezia sympodialis is a dominant commensal fungi in the human skin mycobiome but also associated with common disorders including atopic eczema (AE). M. releases extracellular vesicles, designated MalaEx, which are carriers of small RNAs and allergens, they can induce inflammatory cytokine responses. Here we explored how MalaEx involved host-microbe interactions by comparing protein content that parental yeast cells, investigating cells skin. Cryo-electron tomography revealed...