Richard Cunningham
A5041832272- Epigenetics and DNA Methylation
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Neonatal Respiratory Health Research
- Hippo pathway signaling and YAP/TAZ
- Ferroptosis and cancer prognosis
- Biochemical and Molecular Research
- Antimicrobial Peptides and Activities
- Neuroendocrine Tumor Research Advances
- Aquaculture disease management and microbiota
- Architecture and Computational Design
- RNA and protein synthesis mechanisms
- Wnt/β-catenin signaling in development and cancer
- Advanced Drug Delivery Systems
- Boron Compounds in Chemistry
- Advanced Proteomics Techniques and Applications
- Bioinformatics and Genomic Networks
- Quinazolinone synthesis and applications
- Pharmaceutical and Antibiotic Environmental Impacts
- Chemical Synthesis and Analysis
- Ionic liquids properties and applications
- Biopolymer Synthesis and Applications
- Monoclonal and Polyclonal Antibodies Research
- NF-κB Signaling Pathways
University of Edinburgh
2021-2024
Centre for Inflammation Research
2021-2024
MRC Centre for Regenerative Medicine
2021
Queen's Medical Centre
2021
University of Glasgow
2019-2020
Switch
2019-2020
Cancer Research UK Scotland Institute
2019
Queen's University Belfast
2013-2019
Hôpital Xavier Arnozan
2008
Continuing recalcitrance to therapy cements pancreatic cancer (PC) as the most lethal malignancy, which is set become second leading cause of death in our society. The study aim was investigate association between DNA damage response (DDR), replication stress, and novel therapeutic PC develop a biomarker-driven strategy targeting DDR stress PC.
Pancreatic ductal adenocarcinoma (PDAC) can be divided into transcriptomic subtypes with two broad lineages referred to as classical (pancreatic) and squamous. We find that these are driven by distinct metabolic phenotypes. Loss of genes drive endodermal lineage specification, HNF4A GATA6, switch profiles from predominantly squamous, glycogen synthase kinase 3 beta (GSK3β) a key regulator glycolysis. Pharmacological inhibition GSK3β results in selective sensitivity the squamous subtype;...
EGR2 controls alveolar macrophage function in health and disease.
Tobramycin is a potent antimicrobial aminoglycoside and its effective delivery by encapsulation within nanoparticle carriers could increase activity against infections through combination of sustained release enhanced uptake. Effective therapy clinically relevant model bacteria (Pseudomonas aeruginosa) requires sufficient levels therapeutic drug to maintain concentration above the microbial inhibitory (MIC) bacteria. Previous studies have shown that loading drugs in poly(lactic-co-glycolic)...
Bisphosphonates (BPs) are a class of bone resorptive drug with high affinity for the hydroxyapatite structure matrices that used treatment osteoporosis. However, clinical application is limited by common toxicity, BP-related osteonecrosis jaw. There emerging evidence BPs possess anticancer potential, but exploitation these antiproliferative properties their toxicities. We previously reported utility cationic amphipathic fusogenic peptide, RALA, to traffic anionic nucleic acids into various...
A range of nucleosides have been synthesised utilising a solventless approach to Vorbrüggen glycosylations aided by mechanochemistry.
The Hippo signalling pathway is dysregulated across a wide range of cancer types and, although driver mutations that directly affect the core components are rare, handful found within pleural mesothelioma (PM). PM deadly disease lining lung caused by asbestos exposure. By pooling largest-scale clinical datasets publicly available, we here interrogate associations between most prevalent and disruption in patients, while assessing correlations with variety markers. This analysis reveals...
Spiramycin, tylosin, bacitracin and virginiamycin are among a group of antibiotic growth promoters that have been banned in the European Union since 1999 Council. This was due to concerns over development resistant bacteria emerging between humans animals with threat antibiotics no longer being able be used effectively treat human infections. A sensitive fast immunochemical method is presented for determination these four simultaneously poultry tissue. The employs methanol extraction...
Herein, we present a computational and experimental study assessing the solubility of tobramycin<bold>1</bold>in series hydrophilic room temperature ionic liquids (RTIL).
ABSTRACT As the core, tumorigenic downstream effectors of Hippo signalling pathway, YAP/TAZ and TEAD family transcription factors represent attractive targets for drug discovery efforts within cancer research. This is particularly true context pleural mesothelioma, in which there are many recent preclinical developments clinical trials evaluating efficacy inhibitors. The range inhibitors have shown great promise, but comparisons their performances so far limited. Here we develop a high...
Abstract To date, very little is known regarding the earliest cellular and molecular events within initial preneoplastic cell (PNC) niche that might determine outcome of PNC development. Here, we combine in vivo live imaging single transcriptomics to investigate immediate changes occur PNCs responding innate immune cells first 24 hours following tumour initiation. We do so using an inducible transgenic zebrafish model initiation, which oncogenic HRAS conditionally expressed epidermis. found...
Abstract Alveolar macrophages are the most abundant in healthy lung where they play key roles homeostasis and immune surveillance against air-borne pathogens. Tissue-specific differentiation survival of alveolar relies on niche-derived factors, such as colony stimulating factor 2 (CSF-2) transforming growth beta (TGF-β). However, nature downstream molecular pathways that regulate identity function their response to injury remains poorly understood. Here, we identify transcriptional EGR2 is...
Abstract The associated publication reports proteogenomic analysis of non-small cell lung cancer (NSCLC), where we identified molecular subtypes with distinct immune evasion mechanisms and therapeutic targets, validated our classification method in separate clinical cohorts. This protocol describes sections the bioinformatics multi-omics data, namely, data processing for panel sequencing, identification cancer- driver-related proteins proteomics proteogenomics search, machine learning-based...
ABSTRACT Continuing recalcitrance to therapy cements pancreatic cancer (PC) as the most lethal malignancy, which is set become second leading cause of death in our society. We interrogated transcriptome, genome, proteome and functional characteristics 61 novel PC patient-derived cell lines define therapeutic strategies targeting DNA damage response (DDR) replication stress. show that faithfully recapitulate epithelial component tumors including previously described molecular subtypes....
The identification of molecularly defined subgroups Pancreatic ductal Adenocarcinoma (PDAC) has the potential to transform clinical practice. There is now a growing consensus that PDAC can be divided into transcriptomic subtypes with 2 broad linages referred as Classical (Pancreatic) and Squamous. We find these two are driven by distinct metabolic phenotypes. Loss genes drive endodermal linage specification, HNF4A GATA6, switch profiles from predominantly Squamous, GSK3B key regulator...
Abstract The associated publication reports proteogenomic analysis of non-small cell lung cancer, where we identified molecular subtypes with distinct immune evasion mechanisms and therapeutic targets, validated our classification method in separate clinical cohorts. This protocol describes histological, tertiary lymphoid structure (TLS), immunohistochemical evaluation samples. Specifically, immunohistochemistry was performed for PD-L1, CD3, CD8 on tumor microarrays (TMAs) derived from...
Abstract The associated publication reports proteogenomic analysis of non-small cell lung cancer (NSCLC), where we identified molecular subtypes with distinct immune evasion mechanisms and therapeutic targets, validated our classification method in separate clinical cohorts. in-depth proteomic pointed to a potential role STK11 inactivation liver-specific signaling subsequent growth mechanisms. This protocol describes vitro validation the downstream effects STK11-AMPK on HNF1A FGL1 liver two lines.
Abstract The associated publication reports proteogenomic analysis of non-small cell lung cancer, where we identified molecular subtypes with distinct immune evasion mechanisms and therapeutic targets, validated our classification method in separate clinical cohorts. This protocol describes the sample preparation mass spectrometry (MS)-based in-depth rapid proteomic analyses tumor biopsy samples. We deployed single-pot solid-phase-enhanced (SP3). For analysis, used TMT labeling, followed by...
Background: Integrated multi-omic analyses revealed 24% of pancreatic cancer (PC) harbor defects in DNA damage response (DDR) and a subgroup demonstrate upregulation replication stress pathways. DDR defective tumors preferentially respond to damaging agents, clinical responses cell cycle inhibitors are seen undefined subgroups, representing novel therapeutic strategies for PC. The aim this study is define refine segments agents targeting