A5003549501- Immune cells in cancer
- Parasites and Host Interactions
- Immune Response and Inflammation
- Reproductive System and Pregnancy
- Neuroinflammation and Neurodegeneration Mechanisms
- Endometriosis Research and Treatment
- Parasite Biology and Host Interactions
- Parasitic Infections and Diagnostics
- IL-33, ST2, and ILC Pathways
- Liver physiology and pathology
- Student Assessment and Feedback
- Parasitic Diseases Research and Treatment
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Parasitic infections in humans and animals
- Phagocytosis and Immune Regulation
- T-cell and B-cell Immunology
- Reflective Practices in Education
- Neonatal Respiratory Health Research
- Liver Disease and Transplantation
- Research on Leishmaniasis Studies
- Child Development and Digital Technology
- Macrophage Migration Inhibitory Factor
- Chemokine receptors and signaling
- Higher Education Learning Practices
University of Edinburgh
2016-2025
Centre for Inflammation Research
2016-2025
MRC Centre for Regenerative Medicine
2021-2025
Ochsner Medical Center
1997-2023
The Queen's Medical Research Institute
2014-2022
Queen's Medical Centre
2021-2022
Medical Research Council
2013-2018
University of Utah
2014-2016
Georgia Southern University
1995-2015
Centre for Immunity, Infection and Evolution
2005-2014
A defining feature of inflammation is the accumulation innate immune cells in tissue that are thought to be recruited from blood. We reveal a distinct process exists which macrophages undergo rapid situ proliferation order increase population density. This inflammatory mechanism occurred during T helper 2 (T(H)2)-related pathologies under control archetypal T(H)2 cytokine interleukin-4 (IL-4) and was fundamental component because exogenous IL-4 sufficient drive through self-renewal. Thus,...
Abstract Peritoneal macrophages are one of the most studied macrophage populations in body, yet composition, developmental origin and mechanisms governing maintenance this compartment controversial. Here we show resident F4/80 hi GATA6 + long-lived, undergo non-stochastic self-renewal retain cells embryonic for at least 4 months mice. However, Ly6C monocytes constitutively enter peritoneal cavity a CCR2-dependent manner, where they mature into short-lived lo MHCII that act, part, as...
Macrophages (MΦs) colonize tissues during inflammation in two distinct ways: recruitment of monocyte precursors and proliferation resident cells. We recently revealed a major role for IL-4 the proliferative expansion MΦs Th2-biased tissue nematode infection. now show that intestinal as well infection is restricted to sites production requires MΦ-intrinsic IL-4R signaling. However, both IL-4Rα–dependent –independent mechanisms contributed MΦ infections. IL-4R–independent was controlled by...
Abstract The proliferation, differentiation and survival of mononuclear phagocytes depend on signals from the receptor for macrophage colony-stimulating factor, CSF1R. mammalian Csf1r locus contains a highly conserved super-enhancer, fms -intronic regulatory element (FIRE). Here we show that genomic deletion FIRE in mice selectively impacts CSF1R expression tissue development specific tissues. Deletion ablates murine embryonic stem cells. ΔFIRE/ΔFIRE lack macrophages embryo, brain microglia...
Abstract Many school‐aged children have experienced the cruelty of bullying. The impact and effects bullying been described extensively in scholarly literature. Unfortunately, has also made contemporary headlines with recent rash school shootings other forms school‐based violence. current body students brings an additional threat to Due their sophisticated use technology, today's are using delivery systems taunt, tease, threaten classmates. Cyberbullying is newest form bullying, it...
Intestinal macrophages (mφ) form one of the largest populations mφ in body and are vital for maintenance gut homeostasis. They have several unique properties derived from local differentiation classical Ly6Chi monocytes, but factors driving this tissue-specific process not understood. Here we used global transcriptomic analysis to identify a homeostatic signature mature colonic that is acquired as they differentiate mucosa. By comparing analogous monocyte found dermis, TGFβ an indispensable...
The nematode Heligmosomoides polygyrus is an excellent model for intestinal helminth parasitism. Infection in mice persists varying lengths of time different inbred strains, with CBA and C57BL/6 being fully susceptible, BALB/c partially so SJL able to expel worms within 2–3 weeks infection. We find that resistance correlates not only the adaptive Th2 response, including IL‐10 but activation innate lymphoid cell macrophage populations. In addition, titer specificity range serum antibody...
Autophagy is a catabolic process involved in maintaining energy and organelle homeostasis. The relationship between obesity the regulation of autophagy cell type specific. Despite adverse consequences on cardiac structure function, contribution altered response to fatty acid overload incompletely understood. Here, we report suppression autophagosome clearance activation NADPH oxidase (Nox)2 both high fat-fed murine hearts palmitate-treated H9C2 cardiomyocytes (CMs). Defective secondary...
Abstract Inflammation generally leads to recruitment of monocyte-derived macrophages. What regulates the fate these cells and what extent they can assume identity function resident macrophages is unclear. Here, we show that elicited into peritoneal cavity during mild inflammation persist long-term but are retained in an immature transitory state differentiation due presence enduring By contrast, severe results ablation a protracted phase wherein incapable sustaining phenotype, yet ultimately...
Significance Endometriosis is a chronic, incurable inflammatory disorder impacting 190 million women worldwide. Immune cells called macrophages are implicated in promoting endometriosis. Macrophages have different origins and their origin can dictate function. In this study we demonstrate that endometriotic lesion-resident derived from the uterine lining (endometrium), abdominal (peritoneal) cavity, recruited bone-marrow precursors (monocytes). triggers continuous recruitment of monocytes...
Macrophages reside in the body cavities where they maintain serosal homeostasis and provide immune surveillance. Peritoneal macrophages are implicated etiology of pathologies including peritonitis, endometriosis, metastatic cancer; thus, understanding factors that govern their behavior is vital. Using a combination fate mapping techniques, we have investigated impact sex age on murine peritoneal macrophage differentiation, turnover, function. We demonstrate sexually dimorphic replenishment...
Abstract Hypoxemia is a defining feature of acute respiratory distress syndrome (ARDS), an often-fatal complication pulmonary or systemic inflammation, yet the resulting tissue hypoxia, and its impact on immune responses, often neglected. In present study, we have shown that ARDS patients were hypoxemic monocytopenic within first 48 h ventilation. Monocytopenia was also observed in mouse models hypoxic lung injury, which hypoxemia drove suppression type I interferon signaling bone marrow....
Costimulatory cross-talk can occur at multiple cellular levels to potentiate expansion and polarization of Th responses. Although OX40L ligand (OX40L) is thought play a key role in Th2 development, the critical source this molecule has yet be identified. In study, we demonstrate that expression by initiating dendritic cell (DC) fundamental requirement for optimal induction primary memory responses vivo. Analysis kinetics residual response primed OX40L-deficient DC suggested failure stimulate...
Interleukin-4 is a cytokine widely known for its role in CD4 + T cell polarization and ability to alternatively activate macrophage populations. In contrast, the impact of IL-4 on activation function dendritic cells (DCs) poorly understood. We report here that DCs respond both vitro vivo by expression multiple alternative markers with different pattern macrophages. further demonstrate central DC IL-4Rα optimal induction IFNγ responses Th1 Th2 settings, through feedback loop which promotes...