A5101843679- Carcinogens and Genotoxicity Assessment
- Indoor Air Quality and Microbial Exposure
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Heavy Metal Exposure and Toxicity
- Cancer, Hypoxia, and Metabolism
- COVID-19 Clinical Research Studies
- Immune cells in cancer
- Respiratory Support and Mechanisms
- Air Quality and Health Impacts
- Sepsis Diagnosis and Treatment
- Hemoglobin structure and function
- Heavy metals in environment
- Pesticide Exposure and Toxicity
- Immune Response and Inflammation
- Metabolism and Genetic Disorders
- Effects and risks of endocrine disrupting chemicals
- S100 Proteins and Annexins
- Immunotoxicology and immune responses
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Chromium effects and bioremediation
- Mining and Resource Management
- Tailings Management and Properties
- Pediatric health and respiratory diseases
- Chemical Safety and Risk Management
- Glutathione Transferases and Polymorphisms
University of Edinburgh
2017-2024
Centre for Inflammation Research
2017-2023
The Queen's Medical Research Institute
2020-2023
Queen's Medical Centre
2020-2022
Medical Research Council
2019
National Institute of Health Dr. Ricardo Jorge
2007-2018
Universidade do Porto
2011-2013
Nova Medical (United States)
2008
Neutrophils can function and survive in injured infected tissues, where oxygen metabolic substrates are limited. Using radioactive flux assays LC-MS tracing with U-13C glucose, glutamine, pyruvate, we observe that neutrophils require the generation of intracellular glycogen stores by gluconeogenesis glycogenesis for effective survival bacterial killing. These adaptations dynamic, net increases observed following LPS challenge or altitude-induced hypoxia. from patients chronic obstructive...
Rationale: COPD (Chronic Obstructive Pulmonary Disease) is a disease characterized by persistent airway inflammation and disordered macrophage function. The extent to which alterations in bioenergetics contribute impaired antioxidant responses pathogenesis has yet be fully delineated. Objectives: Through the study of alveolar (AM) peripheral monocyte-derived (MDM) macrophages, we sought establish if intrinsic defects core metabolic processes drive dysfunction redox imbalance. Methods: AM MDM...
Abstract Hypoxemia is a defining feature of acute respiratory distress syndrome (ARDS), an often-fatal complication pulmonary or systemic inflammation, yet the resulting tissue hypoxia, and its impact on immune responses, often neglected. In present study, we have shown that ARDS patients were hypoxemic monocytopenic within first 48 h ventilation. Monocytopenia was also observed in mouse models hypoxic lung injury, which hypoxemia drove suppression type I interferon signaling bone marrow....
Mining activities may affect the health of miners and communities living near mining sites, these effects persist even when mine is abandoned. During processes various toxic wastes are produced released into surrounding environment, resulting in contamination air, drinking water, rivers, plants, soils. In a geochemical sampling campaign undertaken Panasqueira Mine area central Portugal, an anomalous distribution several metals arsenic (As) was identified environmental media. Several...
Formaldehyde (FA) is a commonly used chemical in anatomy and pathology laboratories as tissue preservative fixative. Because of its sensitising properties, irritating effects cancer implication, FA accounts probably for the most important chemical-exposure hazard concerning this professional group. Evidence genotoxic carcinogenic properties humans insufficient conflicting, particularly regard to ability inhaled induce toxicity on other cells besides first contact tissues, such buccal nasal...
Widespread use of pesticides in agriculture represents a threat not only to the environment but also human populations exposed them. Many these compounds are capable inducing mutations DNA and lead several diseases including cancer. In present study, cytogenetic damage peripheral lymphocytes from 33 farmers Oporto district (Portugal) was evaluated by means micronuclei (MN), sister chromatid exchange (SCE) chromosomal aberrations (CA). addition, effect polymorphic genes xenobiotic...
Background: Acute respiratory distress syndrome (ARDS) is a severe critical condition with high mortality that currently in focus given it associated caused by coronavirus disease 2019 (COVID-19). Neutrophils play key role the lung injury characteristic of non-COVID-19 ARDS and there also accumulating evidence neutrophil mediated patients who succumb to infection acute 2 (SARS-CoV-2). Methods: We undertook functional proteomic metabolomic survey circulating populations, comparing COVID-19...
<ns3:p><ns3:bold>Background: </ns3:bold>Acute respiratory distress syndrome (ARDS) is a severe critical condition with high mortality that currently in focus given it associated caused by coronavirus disease 2019 (COVID-19). Neutrophils play key role the lung injury characteristic of non-COVID-19 ARDS and there also accumulating evidence neutrophil mediated patients who succumb to infection acute 2 (SARS-CoV-2).</ns3:p><ns3:p> <ns3:bold>Methods: </ns3:bold>We undertook functional proteomic...
Limiting dysfunctional neutrophilic inflammation while preserving effective immunity requires a better understanding of the processes that dictate neutrophil function in tissues. Quantitative mass-spectrometry identified how inflammatory murine neutrophils regulated expression cell surface receptors, signal transduction networks, and metabolic machinery to shape phenotypes response hypoxia. Through tracing labeled amino acids into enzymes, proinflammatory mediators, granule proteins, we...
Abstract Formaldehyde (FA) is a world high-production compound with numerous applications ranging from production of resins to medicines. Due its sensitizing properties, irritating effects and potential cancer hazard FA great environmental health concern. Numerous studies in humans experimental animals demonstrated that inhaled produced toxicity, genotoxicity, at distal sites. IARC, based on sufficient data, reclassified as human carcinogen. The highest level exposure this aldehyde occurs...
Acute respiratory distress syndrome is defined by the presence of systemic hypoxia and consequent on disordered neutrophilic inflammation. Local mechanisms limiting duration magnitude this response remain poorly understood.
Formaldehyde (FA) is a widely used industrial chemical for which exposure associated with nasopharyngeal and sinonasal cancer. Based on sufficient evidence of carcinogenicity from human investigations, supporting studies mechanisms underlying carcinogenesis, experimental in animals, FA status was recently revised reclassified as carcinogen. The highest level to occurs occupational settings. Although several reported ability induce genotoxic responses exposed workers, not all findings were...
Neutrophilic inflammation is central to disease pathogenesis, for example, in chronic obstructive pulmonary disease, yet the mechanisms that retain neutrophils within tissues remain poorly understood. With emerging evidence axon guidance factors can regulate myeloid recruitment and expression of a class 3 semaphorin, SEMA3F, we investigated role SEMA3F inflammatory cell retention inflamed tissues. We observed upregulate response proinflammatory mediators following neutrophil lung. In both...
Styrene is a widely used chemical in the manufacture of synthetic rubber, resins, polyesters, and plastics. The highest levels human exposure to styrene occur during production reinforced plastic products. objective this study was examine occupational multistage approach, order integrate following endpoints: workplace air, mandelic phenylglyoxylic acids (MA + PGA) urine, sister chromatid exchanges (SCE), micronuclei (MN), DNA damage (comet assay), genetic polymorphisms metabolizing enzymes...
Neutrophils are essential in the early innate immune response to pathogens. Harnessing their antimicrobial powers, without driving excessive and damaging inflammatory responses, represents an attractive therapeutic possibility. The neutrophil population is increasingly recognised be more diverse malleable than was previously appreciated. Hypoxic signalling pathways known regulate important behaviours and, as such, potential targets for regulating responses.