A5043581887- Immune cells in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Photodynamic Therapy Research Studies
- IL-33, ST2, and ILC Pathways
- Ocular Infections and Treatments
- Parasites and Host Interactions
- Ocular Surface and Contact Lens
- Galectins and Cancer Biology
- Nanoplatforms for cancer theranostics
- Immune Cell Function and Interaction
- Biosensors and Analytical Detection
- Genetics, Bioinformatics, and Biomedical Research
- MicroRNA in disease regulation
- Gut microbiota and health
- Photoacoustic and Ultrasonic Imaging
- Autoimmune and Inflammatory Disorders Research
- Ocular Diseases and Behçet’s Syndrome
- Parasitic Diseases Research and Treatment
- Immune responses and vaccinations
- Antibiotic Resistance in Bacteria
- Corneal Surgery and Treatments
- Biotechnology and Related Fields
- Respiratory viral infections research
- Atomic and Subatomic Physics Research
- COVID-19 Impact on Reproduction
University of Edinburgh
2012-2024
Centre for Inflammation Research
2013-2024
Medical Research Institute
2020
Queen's Medical Centre
2020
Engineering and Physical Sciences Research Council
2020
Centre for Immunity, Infection and Evolution
2012-2017
Institute of Immunology
2013-2016
Roslin Institute
2013
Medical Research Council
2013
Macrophages (MΦs) colonize tissues during inflammation in two distinct ways: recruitment of monocyte precursors and proliferation resident cells. We recently revealed a major role for IL-4 the proliferative expansion MΦs Th2-biased tissue nematode infection. now show that intestinal as well infection is restricted to sites production requires MΦ-intrinsic IL-4R signaling. However, both IL-4Rα–dependent –independent mechanisms contributed MΦ infections. IL-4R–independent was controlled by...
Fat-associated lymphoid clusters (FALC) are inducible structures that support rapid innate-like B-cell immune responses in the serous cavities. Little is known about physiological cues activate FALCs pleural cavity and more generally mechanisms controlling activation FALCs. Here we show, using separate models of nematode infection with Litomosoides sigmodontis Altenaria alternata induced acute lung inflammation, inflammation rapidly activates mediastinal pericardial IL-33 produced by FALC...
The emergence of multidrug resistant (MDR) pathogens and the scarcity new potent antibiotics antifungals are one biggest threats to human health. Antimicrobial photodynamic therapy (aPDT) combines light photosensitizers kill drug-resistant pathogens; however, there limited materials that can effectively ablate different classes infective pathogens. In present work, a class benzodiazole-paired is designed as highly PDT agents with broad-spectrum antimicrobial activity upon illumination...
Ym1 and RELMα are established effector molecules closely synonymous with Th2-type inflammation associated pathology. Here, we show that whilst largely dependent on IL-4Rα signaling during a type 2 response, also have IL-4Rα-independent expression patterns in the lung. Notably, found has opposing effects immunity nematode infection depending whether it is expressed at time of innate or adaptive responses. During lung migratory stage Nippostrongylus brasiliensis, promoted subsequent reparative...
The methylene blue-polymyxin conjugate demonstrated high selectivity, sensitivity and phototoxicity against Gram-negative bacteria, including in early biofilm models.
Rapid reprogramming of the macrophage activation phenotype is considered important in defense against consecutive infection with diverse infectious agents. However, setting persistent, chronic functional importance macrophage-intrinsic adaptation to changing environments vs. recruitment new macrophages remains unclear. Here we show that resident peritoneal expanded by nematode Heligmosomoides polygyrus bakeri altered their response Salmonella enterica ser. Typhimurium vitro and vivo. The...
IL‐33 plays an important role in the initiation of type‐2 immune responses, as well enhancement type 2 effector functions. Engagement receptor on macrophages facilitates polarization to alternative activation state by amplifying IL‐4 and IL‐13 signaling IL‐4Rα. also induce macrophage proliferation but involvement this process has not been rigorously evaluated. As expected, vivo delivery induced IL‐4Rα‐dependent serous cavities. proliferate but, unexpectedly, was independent IL‐4Rα signaling....
The relentless rise in antimicrobial resistance is a major societal challenge and requires, as part of its solution, better understanding bacterial colonization infection. To facilitate this, we developed highly efficient no-wash red optical molecular imaging agent that enables the rapid, selective, specific visualization Gram-positive bacteria through bespoke fiber-based delivery/imaging endoscopic device.We rationally designed no-wash, red, Gram-positive-specific (Merocy-Van) based on...
Microbial keratitis (MK) is a common cause of unilateral visual impairment, blindness, and eye loss in low-income middle-income countries. There an urgent need to develop implement rapid simple point-of-care diagnostics for MK increase the likelihood good outcomes.
Purpose: Rapid and accurate diagnosis of microbial keratitis (MK) could greatly improve patient outcomes. Here, we present the development a rapid, accessible multicolour fluorescence imaging device (FluoroPi) evaluate its performance in combination with fluorescent optical reporters (SmartProbes) to distinguish bacterial Gram status. Furthermore, show feasibility by samples obtained corneal scrape minimally invasive impression membrane (CIM) from ex vivo porcine MK models. Methods: FluoroPi...
Endoscopic point sensing of bacterial infections in the distal lung vivo is challenging. Here fluorescence imaging agents are combined with time-resolved spectroscopy to overcome limitations from tissue by measuring lifetime.
Infections with the hookworm <i>Nippostrongylus brasiliensis</i> cause a rapid phase of pulmonary damage, immune activation, wound healing and then long term progressive emphysematous pathology in mice. The emphysema is visible one month after has left lungs was described but not further investigated 2008. We characterized immunological landscape from infection, to investigate which cell types activation pathways that are necessary for by comparing BALB/c, C57BL/6 mice, IL-4Rα<sup>-/-</sup>...
Infections with the hookworm <i>Nippostrongylus brasiliensis</i> cause a rapid phase of pulmonary damage, immune activation, wound healing and then long term progressive emphysematous pathology in mice. The emphysema is visible one month after has left lungs was described but not further investigated 2008. We characterized immunological landscape from infection, to investigate which cell types activation pathways that are necessary for by comparing BALB/c, C57BL/6 mice, IL-4Rα<sup>-/-</sup>...
Fluorescence imaging and 'smart probes' are an emerging point-of-care platform for microbial detection. This article proposes a proof-of-concept frugal fluorescence system the detection of bacteria such as Pseudomonas aeruginosa, Escherichia coli Staphylococcus aureus. In this work, we investigate capability trans-illuminating to detect using low-cost raspberry pi single board computer high-quality camera. is capable producing high quality transmission images bacterial samples with submicron...