A5042944165- Prion Diseases and Protein Misfolding
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Advanced biosensing and bioanalysis techniques
- Alzheimer's disease research and treatments
- Synthesis and Biological Evaluation
- Click Chemistry and Applications
- Molecular Junctions and Nanostructures
- Analytical Chemistry and Sensors
- HIV Research and Treatment
- Microwave-Assisted Synthesis and Applications
- Advanced Biosensing Techniques and Applications
- Nanoplatforms for cancer theranostics
- Trace Elements in Health
- Photodynamic Therapy Research Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Catalytic C–H Functionalization Methods
- Cell Adhesion Molecules Research
- Supramolecular Self-Assembly in Materials
- Catalytic Cross-Coupling Reactions
- Multicomponent Synthesis of Heterocycles
- Biotin and Related Studies
- Synthesis and biological activity
- Polymer Surface Interaction Studies
- Research on Leishmaniasis Studies
Universidad Complutense de Madrid
2012-2024
University of Edinburgh
2017-2023
Brewster Place
2020
Engineering and Physical Sciences Research Council
2020
Instituto de Química Orgánica General
2010
Peptide-based electrochemical biosensors typically consist of a short peptide sequence, labelled with redox reporter and modified thiol-containing moiety to allow immobilisation onto gold electrode surface. A spacer is often introduced between the thiol group aim promoting enzyme accessibility as well conferring flexibility probe. Herein we report systematic study effect polyethylene glycol (PEG)-based length on performance such in order gain deeper understanding their role optimise...
The methylene blue-polymyxin conjugate demonstrated high selectivity, sensitivity and phototoxicity against Gram-negative bacteria, including in early biofilm models.
(E)-6-Methyl-4'-amino-2-styrylquinoline (3) is a small molecule with the proper features to potentially diagnose, deliver therapy and monitor response in protein misfolding diseases. These include compound fluorescent emission NIR region its ability interact both Aβ prion fibrils, staining them high selectivity. Styrylquinoline 3 also inhibits self-aggregation vitro replication submicromolar range cellular context. Furthermore, it not toxic able cross blood brain barrier (PAMPA test).
Prion diseases are fatal neurodegenerative and infectious disorders for which effective pharmacological tools not yet available. This unmet challenge the recently proposed interplay between prion Alzheimer's have led to a more urgent demand new antiprion agents. Herein, we report identification of novel bifunctional diketopiperazine (DKP) derivative 1 d, exhibits activity in low micromolar range against replication ScGT1 cells, while showing cytotoxicity. Supported by properly addressed...
Human neutrophil elastase (HNE) is a serine protease, produced by polymorphonuclear neutrophils (PMNs), whose uncontrolled production has been associated with various inflammatory disease states as well tumour proliferation and metastasis. Here we report the development characterisation of an electrochemical peptide-based biosensor, which enables detection clinically relevant levels HNE. The sensing platform was characterised in terms its analytical performance, enzymatic cleavage kinetics...
Proteases are ideal target biomarkers as they have been implicated in many disease states, including steps associated with cancer progression. Electrochemical peptide-based biosensors attracted much interest recent years. However, the significantly large size of electrodes typically used most these platforms has led to performance limitations. These could be addressed by enhancements offered microelectrodes, such rapid response times, improved mass transport, higher signal-to-noise and...
Abstract Focused microwave irradiation of a series halogenated nitrogen heterocycles and different kinds nucleophiles in the presence catalytic amount indium trichloride leads to efficient completely regioselective generation aromatic CC CN bonds. The method is simple, rapid, general inexpensive, can be performed without use dried solvents. Most synthetized compounds are new many cases work‐up required only filtration. Furthermore, this first example Lewis acid as catalyst for...
The vinyl ether benzyloxycarbonyl (VeZ) protecting group is selectively cleaved by treatment with tetrazines via an inverse electron-demand Diels–Alder reaction. This represents a new orthogonal for solid-phase peptide synthesis, Fmoc-Lys(VeZ)-OH as versatile alternative to Fmoc-Lys(Alloc)-OH and Fmoc-Lys(Dde)-OH, demonstrated the synthesis of two biologically relevant cyclic peptides.
Controlled activation of a Pt(<sc>iv</sc>) prodrug into its active Pt(<sc>ii</sc>) form within cellular context, using an electrochemical strategy.
Peptide-based electrochemical biosensors have proven to be powerful sensing strategies for the detection of proteases and offer a highly versatile system, as simple tuning immobilised substrate peptide leads new platform. The most common immobilisation strategy peptides onto an electrode surface is via self-assembled monolayer (SAM) through thiol group that can readily chemically incorporated in target peptide. However, successful application these platforms complex biological samples...
Prion diseases are characterized by the self-assembly of pathogenic misfolded scrapie isoforms (PrPSc) cellular prion protein (PrPC). In an effort to achieve a theranostic profile, symmetrical bifunctional carbazole derivatives were designed as fluorescent rigid analogues GN8, pharmacological chaperone that stabilizes native PrPC conformation and prevents its conversion. A focused library was synthesized via four-step route, representative member confirmed have fluorescence, including band...
Implantable electrochemical sensors that enable the real-time detection of significant biomarkers offer huge potential for enhancement and personalisation therapies; however, biofouling is a key challenge encountered by any implantable system. This particularly an issue immediately after implantation, when foreign body response associated processes are at their most active in passivating object. Here, we present development sensor protection activation strategy against biofouling, based on...
Focused microwave irradiation promoted the very efficient synthesis of 2-styrylquinolines by reaction between quinaldines and benzaldehydes or cinnamaldehydes in presence acetic anhydride.
A group of functionalized fluorene derivatives that are structurally similar to the cellular prion protein ligand N,N′-(methylenedi-4,1-phenylene)bis [2-(1-pyrrolidinyl)acetamide] (GN8) have been synthesized. These compounds show remarkable native fluorescence due ring. The substituents introduced at positions 2 and 7 moiety sufficiently flexible accommodate beta-conformational folding develops in amyloidogenic proteins. Changes these provide evidence transformations aggregation processes...
Abstract By the microwave irradiation of chlorinated N‐heterocycles in presence nucleophiles aromatic C—C or C—N bonds are generated regioselectively.