The transport and trafficking of metabolites are critical for the correct functioning live cells. However, in situ metabolic imaging studies hampered by lack fluorescent chemical structures that allow direct monitoring small under physiological conditions with high spatial temporal resolution. Herein, we describe SCOTfluors as novel small-sized multi-colored fluorophores real-time tracking essential cells vivo acquisition profiles from human cancer variable origin.

Microglia, the innate immune cells of central nervous system, actively participate in brain development by supporting neuronal maturation and refining synaptic connections. These are emerging as highly metabolically flexible, able to oxidize different energetic substrates meet their energy demand. Lactate is particularly abundant brain, but whether microglia use it a metabolic fuel has been poorly explored. Here we show that can import lactate, this coupled with increased lysosomal...

Abstract Photodynamic therapy is an anti‐cancer treatment that requires illumination of photosensitizers to induce local cell death. Current near‐infrared organic are built from large and non‐modular structures cannot be tuned improve safety minimize off‐target toxicity. This work describes a novel chemical platform generate enzyme‐activatable photosensitizers. We optimized the Se‐bridged hemicyanine scaffold include caging groups biocompatible moieties, generated cathepsin‐triggered for...

The emergence of multidrug resistant (MDR) pathogens and the scarcity new potent antibiotics antifungals are one biggest threats to human health. Antimicrobial photodynamic therapy (aPDT) combines light photosensitizers kill drug-resistant pathogens; however, there limited materials that can effectively ablate different classes infective pathogens. In present work, a class benzodiazole-paired is designed as highly PDT agents with broad-spectrum antimicrobial activity upon illumination...

The State 1 to 2 transition in the photosynthetic membranes of plants and green algae involves functional coupling phosphorylated light‐harvesting complexes photosystem II (LHCII) I (PSI). We present evidence suggesting that Chlamydomonas reinhardtii this may be aided by a hyper‐phosphorylated form LHCII‐like CP29 protein (Lhcbm4). MS analysis showed Thr6, Thr16 Thr32, Ser102 are 2, whereas 1‐exposed cells only phosphorylation Thr6 Thr32 could detected. LHCI–PSI supercomplex isolated from...

Fungal infections caused by Candida species are among the most prevalent in hospitalized patients. However, current methods for detection of fungal cells clinical samples rely on time-consuming assays that hamper rapid and reliable diagnosis. Herein, we describe rational development new Phe-BODIPY amino acids as small fluorogenic building blocks their application to generate fluorescent antimicrobial peptides labelling urine. We have used computational analyse behaviour BODIPY-substituted...

Abstract Photoactivatable molecules enable ablation of malignant cells under the control light, yet current agents can be ineffective at early stages disease when target are similar to healthy surrounding tissues. In this work, we describe a chemical platform based on amino-substituted benzoselenadiazoles build photoactivatable probes that mimic native metabolites as indicators onset and progression. Through series synthetic derivatives, have identified key groups in benzoselenadiazole...

Cytotoxic immune cells, including T lymphocytes (CTLs) and natural killer (NK) are essential components of the host response against tumors. CTLs NK cells secrete granzyme A (GzmA) upon recognition cancer cells; however, there very few tools that can detect physiological levels active GzmA with high spatiotemporal resolution. Herein, we report rational design near-infrared fluorogenic substrates for human mouse GzmA. These activity-based probes display catalytic efficiency selectivity over...

The essential functions that cytokine/immune cell interactions play in tissue homeostasis and during disease have prompted the molecular design of targeted fluorophores to monitor their activity real time. Whereas activatable probes for imaging immune-related enzymes are common, many immunological mediated by binding events between cytokines cognate receptors hard live-cell imaging. A prime example is interleukin-33 (IL-33), a key cytokine innate adaptive immunity, whose interaction with ST2...

Abstract The transport and trafficking of metabolites are critical for the correct functioning live cells. However, in situ metabolic imaging studies hampered by lack fluorescent chemical structures that allow direct monitoring small under physiological conditions with high spatial temporal resolution. Herein, we describe SCOTfluors as novel small‐sized multi‐colored fluorophores real‐time tracking essential cells vivo acquisition profiles from human cancer variable origin.

Bacterial infections remain among the biggest challenges to human health, leading high antibiotic usage, morbidity, hospitalizations, and accounting for approximately 8 million deaths worldwide every year.The overuse of antibiotics paucity antimicrobial innovation has led resistant pathogens that threaten reverse key advances modern medicine.Photodynamic therapeutics can kill bacteria but there are few agents ablate with minimal off-target effects.Methods: We describe...

Abstract Controlling the succession of chemical processes with high specificity in complex systems is advantageous for widespread applications, from biomedical research to drug manufacturing. Despite synthetic advances bioorthogonal and photochemical methodologies, there a need generic approaches that can universally modulate photodynamic reactivity organic photosensitizers. Herein we present strategy fine-tune production singlet oxygen multiple photosensitive scaffolds under activation...

Charting the chemical reaction space around combination of carbonyls, amines, and isocyanoacetates allows description new multicomponent processes leading to a variety unsaturated imidazolone scaffolds. The resulting compounds display chromophore green fluorescent protein core natural product coelenterazine. Despite competitive nature pathways involved, general protocols provide selective access desired chemotypes. Moreover, we describe unprecedented reactivity at C-2 position directly...

Abstract Photodynamic therapy is an anti‐cancer treatment that requires illumination of photosensitizers to induce local cell death. Current near‐infrared organic are built from large and non‐modular structures cannot be tuned improve safety minimize off‐target toxicity. This work describes a novel chemical platform generate enzyme‐activatable photosensitizers. We optimized the Se‐bridged hemicyanine scaffold include caging groups biocompatible moieties, generated cathepsin‐triggered for...

Abstract The development of fluorophores emitting in the near-infrared spectral window has gained increased attention given their suitable features for biological imaging. In this work, we have optimised a general and straightforward synthetic approach to prepare small library near-infrared-emitting C-bridged nitrobenzodiazoles using commercial precursors. benzodiazoles low molecular weight neutral character as important that are not common most dyes. We investigated fluorescence response...

Abstract Pilzinfektionen, die durch Candida‐Arten verursacht werden, gehören zu den häufigsten Infektionen bei Krankenhauspatienten. Die derzeitigen Methoden zum Nachweis von Candida‐Pilzzellen in klinischen Proben beruhen jedoch auf zeitaufwändigen Analysen, eine schnelle und zuverlässige Diagnose erschweren. In diesem Beitrag beschreiben wir rationale Entwicklung neuer Phe‐BODIPY‐Aminosäuren als kleine fluorogene Bausteine ihre Anwendung zur Erzeugung fluoreszierender antimikrobieller...

Charting the chemical reaction space around combination of carbonyls, amines, and isocyanoacetates allows description new multicomponent processes leading to a variety unsaturated imidazolone scaffolds. The resulting compounds display chromophore green fluorescent protein core natural product coelenterazine. Despite competitive nature pathways involved, general protocols provide selective access desired chemotypes. Moreover, we describe unprecedented reactivity at C-2 position directly...

Photoswitchable fluorescent molecules (PSFMs) are positioned as valuable tools for biomolecule localization tracking and super-resolution imaging technologies due to their unique ability reversibly control fluorescence intensity upon light irradiation. Despite the high demand PSFMs that suitable live-cell imaging, no general method has been reported enables reversible on proteins of interest in living cells. Herein, we have established a platform realize switching cells by adapting protein...

Abstract Recent advances in optical bioimaging have prompted the need for minimal chemical reporters that can retain molecular recognition properties and activity profiles of biomolecules. As a result, several methodologies to reduce size fluorescent Raman labels few atoms (e.g., single aryl fluorophores, Raman‐active triple bonds isotopes) embed them into building blocks amino acids, nucleobases, sugars) construct native‐like supramolecular structures been described. The integration small...

Cytotoxic immune cells, including T lymphocytes (CTLs) and natural killer (NK) are essential components of the host response against tumors. CTLs NK cells secrete granzyme A (GzmA) upon recognition cancer cells; however, there very few tools that can detect physiological levels active GzmA with high spatiotemporal resolution. Herein, we report rational design near-infrared fluorogenic substrates for human mouse GzmA. These activity-based probes display catalytic efficiency selectivity over...

Photoactivatable molecules enable ablation of malignant cells under the control light, yet current agents can be ineffective at early stages disease when target are similar to healthy surrounding tissues. In this work, we describe a chemical platform based on amino-substituted benzoselenadiazoles build photosensitizers that mimic native metabolites as indicators onset and progression. Through series synthetic derivatives, have identified key groups in benzoselenadiazole scaffold responsible...

Siderite and shale nodules from the Monongalia Group Upper Pennsylvanian of Doddridge County, WV have produce a flora fauna similar to Mazon Creek, IL Lagerstatte. Hundreds specimens yielded mostly individual pinnules Neuropteris, Pecopteris Alethopteris species. A few dozen Paleocaris Order: (Decapoda) an unidentified horseshoe crab (Order: Xiphosura) some other but presumed insects. This new locality is stratigraphically higher than Creek fauna.