A5100418710- Click Chemistry and Applications
- Luminescence and Fluorescent Materials
- Molecular Sensors and Ion Detection
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Chemical Synthesis and Analysis
- Computational Drug Discovery Methods
- Alzheimer's disease research and treatments
- Geotechnical Engineering and Underground Structures
- Neuroscience and Neuropharmacology Research
- Magnetic Bearings and Levitation Dynamics
- Advanced Chemical Sensor Technologies
- RNA Interference and Gene Delivery
- Crystallization and Solubility Studies
- Biotin and Related Studies
- X-ray Diffraction in Crystallography
- Grouting, Rheology, and Soil Mechanics
- Protein Degradation and Inhibitors
- Biochemical Analysis and Sensing Techniques
- Photoreceptor and optogenetics research
- Insect-Plant Interactions and Control
- Structural Behavior of Reinforced Concrete
- Railway Engineering and Dynamics
- Near-Field Optical Microscopy
- Lipid Membrane Structure and Behavior
Korea University
2010-2025
Chungnam National University
2024-2025
Gyeongsang National University
2021-2024
Chonnam National University
2017-2023
Sangmyung University
2023
National Institute of Food and Drug Safety Evaluation
2020-2023
University of the Ryukyus
2022
Pusan National University
2002-2022
Korea Institute of Science and Technology
2011-2021
Government of the Republic of Korea
2015-2021
Abstract Molecular recognition and discrimination of carbohydrates are important because perform essential roles in most living organisms for energy metabolism cell-to-cell communication. Nevertheless, it is difficult to identify or distinguish various carbohydrate molecules owing the lack a significant distinction physical chemical characteristics. Although there has been considerable effort develop sensing platform individual selectively using receptors an ensemble array, their detection...
Abstract The bioorthogonality of tetrazole photoclick chemistry has been reassessed. Upon photolysis a tetrazole, the highly reactive nitrile imine formed undergoes rapid nucleophilic reaction with variety nucleophiles present in biological system, along expected cycloaddition alkenes. alternative use was thus explored: tetrazoles were incorporated into Bodipy and Acedan dyes, providing novel photo‐crosslinkers one‐ two‐photon fluorescence Turn‐ON properties that may be developed...
Abstract Molecular aggregates are receiving tremendous attention, demonstrating immense potential for biomedical applications in vitro and vivo. For instance, the molecular of conventional fluorophores influence electronic excitation states aggregates, causing characteristic photophysical property changes. A fundamental understanding this classical relationship between aggregate structures photophysics has allowed innovative biological applications. The chemical characteristics drug...
The first BODIPY library (BD) was synthesized, and a highly selective glucagon sensor, Glucagon Yellow (BD-105), discovered by fluorescence image-based screening method. BD synthesized via Knoevenagel-type condensation reaction with 160 benzaldehydes the 1,3 dimethyl-BODIPY scaffold. Using compounds, performed against three cell lines including AlphaTC1 BetaTC6 cells which secret insulin, respectively, HeLa as control cells. Out of candidate probes, one compound, Yellow, exhibited staining...
Abstract As a candidate for rapid detection of biomaterials, terahertz (THz) spectroscopy system can be considered with some advantage in non-destructive, label-free, and non-contact manner. Because protein-ligand binding energy is the THz range, especially, most important conformational information molecular interactions captured by electromagnetic wave. Based on time-domain system, nano-metamaterial sensing chips were prepared great enhancing sensitivity. A metamaterial chip was designed...
A cell is the smallest functional unit of life. All forms life rely on cellular processes to maintain normal functions, and changes in function induced by metabolic disturbances, physicochemical damage, infection, or abnormal gene expression may cause disease. To understand basic biology develop therapeutics for diseases, researchers need study live cells. Along with advances fluorescence microscopy vitro culture, live-cell imaging has become an essential tool modern molecular events....
Abstract The design of the first dual‐purpose activity‐based probe monoamine oxidase B (MAO‐B) is reported. This highly selective towards MAO‐B, even at high MAO‐A expression levels, and could sensitively report endogenous MAO‐B activities by both in situ proteome profiling live‐cell bioimaging. With a built‐in imaging module as part design, was able to accomplish what all previously reported probes failed do thus far: without encountering diffusion problems.
Abstract Target‐identification phenotypic screening has been a powerful approach in drug discovery; however, it is hindered by difficulties identifying the underlying cellular targets. To address this challenge, we have combined of fully functionalized small‐molecule library with competitive affinity‐based proteome profiling to map and functionally characterize targets hits. Using approach, identified ANXA2, PDIA3/4, FLAD1, NOS2 as primary two bioactive molecules that inhibit cancer cell...
A proper intracellular delivery method with target tissue specificity is critical to utilize the full potential of therapeutic molecules including siRNAs while minimizing their side effects. Herein, we prepare four small-sized DNA tetrahedrons (sTds) by self-assembly different sugar backbone-modified oligonucleotides and screened them develop a platform for kidney-targeted cytosolic siRNA. An in vivo biodistribution study revealed kidney-specific accumulation mirror tetrahedron (L-sTd). Low...
The combinatorial approach: A library of fluorescent styryl dyes (320 compounds) was prepared by solid-phase chemistry. were screened for their detection amyloid aggregates, which are associated with diseases such as Alzheimer's, and two the 320 compounds screened, 2C40 2E10, showed promise brain-imaging agents (see microscopy image).
The sweet-test thing! Carbohydrate discrimination is highly challenging as a result of the similarity in their functional groups. A series pH sensor/pH change inducer ensembles has been developed an artificial "tongue" for identification carbohydrates. optimized sensor array with six probe pairs demonstrated full 23 Supporting information this article available on WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z602055_s.pdf or from author. Please note: publisher not responsible...
The cell-by date: fluorescent compound CDy1 selectively stains embryonic stem cells (see scheme). was used to identify fibroblasts undergoing reprogramming induced pluripotent prior the expression of green protein under control Oct4 promoter. Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by authors. Please note: publisher is responsible for content...
Abstract Recent evidence suggests that tau aggregates are not only neurotoxic, but also propagate in neurons acting as a seed for native aggregation. Prion-like transmission is now considered an important pathogenic mechanism driving the progression of pathology brain. However, prion-like species have been clearly characterized. To identify infectious conformers, here we prepared diverse and evaluated effect on inducing intracellular tau-aggregation. Among tested, dimer containing...
Abstract Affinity‐based probes (A f BPs) provide a powerful tool for large‐scale chemoproteomic studies of drug–target interactions. The development high‐quality capable recapitulating genuine engagement, however, could be challenging. “Minimalist” photo‐crosslinkers, which contain an alkyl diazirine group and chemically tractable tag, alleviate such challenges, but few are currently available. Herein, we have developed new diazirine‐containing photo‐crosslinkers with different bioorthogonal...
A better understanding of the cellular and molecular mechanisms involved in reprogramming somatic cells is essential for further improvement induced pluripotent stem (iPS) cell technology. In this study, we enriched actively undergoing at different time points by sorting stained with a cell-selective fluorescent chemical probe CDy1 their global gene expression analysis. Day-to-day comparison differentially expressed genes showed highly dynamic transient expressions during reprogramming,...
Abstract The bioorthogonality of tetrazole photoclick chemistry has been reassessed. Upon photolysis a tetrazole, the highly reactive nitrile imine formed undergoes rapid nucleophilic reaction with variety nucleophiles present in biological system, along expected cycloaddition alkenes. alternative use was thus explored: tetrazoles were incorporated into Bodipy and Acedan dyes, providing novel photo‐crosslinkers one‐ two‐photon fluorescence Turn‐ON properties that may be developed...
As a cell-permeable imaging probe,<bold>BD-tau</bold>selectively labeled pathological tau aggregates in live neuronal cells.