A5048297833- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Advanced Biosensing Techniques and Applications
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- Antibiotics Pharmacokinetics and Efficacy
- Biosensors and Analytical Detection
- Chemical Synthesis and Analysis
- Molecular Sensors and Ion Detection
- High Altitude and Hypoxia
- Sparse and Compressive Sensing Techniques
- Adipose Tissue and Metabolism
- Molecular Biology Techniques and Applications
- RNA regulation and disease
- Metabolomics and Mass Spectrometry Studies
- Pneumonia and Respiratory Infections
- Protein Tyrosine Phosphatases
- Nanoplatforms for cancer theranostics
- Nanopore and Nanochannel Transport Studies
- Quantum Information and Cryptography
Korea Institute of Science and Technology
2015-2024
Korea University of Science and Technology
2015-2024
Bio-Medical Science (South Korea)
2017-2024
Government of the Republic of Korea
2015-2020
Seoul Institute
2018-2020
Biomedical Research Institute
2015-2020
Seoul National University
1999-2020
Mayo Clinic in Florida
2018
WinnMed
2018
Massachusetts Institute of Technology
2015
A generalized platform for introducing a diverse range of biomolecules into living cells in high-throughput could transform how complex cellular processes are probed and analyzed. Here, we demonstrate spatially localized, efficient, universal delivery immortalized primary mammalian using surface-modified vertical silicon nanowires. The method relies on the ability nanowires to penetrate cell’s membrane subsequently release surface-bound molecules directly cytosol, thus allowing highly...
A DNA tetrahedron is employed for efficient delivery of doxorubicin into drug-resistant breast cancer cells. The drug delivered with the nanoconstruct considerably cytotoxic, whereas free virtually non-cytotoxic Thus, tetrahedron, made inherently natural and biocompatible material, can be a good candidate carrier to overcome MDR in
Despite the promising photofunctionalities, phosphorescent probes have been examined only to a limited extent, and molecular features that provide convenient handles for controlling phosphorescence response yet be identified. We synthesized series of zinc sensors based on cyclometalated heteroleptic Ir(III) complex. The sensor construct includes two anionic cyclometalating ligands neutral diimine ligand tethers di(2-picolyl)amine (DPA) receptor. A with range electron densities band gap...
Reversible regulation of enzyme activity by chemical and physical stimuli is often achieved incorporating stimuli-responsive domains in the interest. However, this method suitable for a limited number enzymes with well-defined structural conformational changes. In study, we present to encapsulate DNA cage that could transform its conformation depending on pH, allowing reversible control accessibility surrounding environment. This enabled us regulate various properties enzyme, such as...
A proper intracellular delivery method with target tissue specificity is critical to utilize the full potential of therapeutic molecules including siRNAs while minimizing their side effects. Herein, we prepare four small-sized DNA tetrahedrons (sTds) by self-assembly different sugar backbone-modified oligonucleotides and screened them develop a platform for kidney-targeted cytosolic siRNA. An in vivo biodistribution study revealed kidney-specific accumulation mirror tetrahedron (L-sTd). Low...
The protein corona is a layer formed on the surface of nanoparticles administered in vivo and considerably affects fate nanoparticles. Although it challenging to control adsorption precisely, may be harnessed develop targeted drug delivery system if are decorated with ligand enhanced affinity target tissue- cell-homing proteins. Here, we prepared DNA tetrahedron trivalent cholesterol conjugation (Chol3-Td) that can induce interaction lipoproteins serum, which situ generates...
Abstract Cell penetrating peptides (CPPs) are that can be translocated into cells and used as a carrier platform for the intracellular uptake of cargo molecules. Subject to source CPP sequences their positively charged nature, cytotoxicity immunogenicity conventional CPPs needs optimized expand utility biomedical applications. In addition these safety issues, stability addressed since residues prone interact with biological milieu. As an effort overcome limitations current technology, we...
Abstract In this study, Cas9 system was employed to down-regulate mdr1 gene for overcoming multidrug resistance of cancer cells. Disruption the MDR1 achieved by delivery Cas9-sgRNA plasmid or ribonucleoprotein complex using a conventional transfection agent and protein transduction domain (PTD). Doxorubicin showed considerable cytotoxicity drug-resistant breast cells pre-treated with RNA-guided endonuclease (RGEN) systems, whereas virtually non-toxic untreated The potency drug enhanced in...
DNA nanoconstructs are a potential drug carrier with high biocompatibility. They promising particularly when therapeutic nucleic acids the cargos to be delivered, since both and cargo which can designed, synthesized assembled in seamless feature without using post-synthetic conjugation chemistry. However, unwanted base-paring events between may potentially disturb desired structure of cargo-loaded carrier. To address this concern, we propose nanocarrier composed L-DNA strands having...
Photodynamic therapy (PDT) is a cytotoxic treatment using singlet oxygen produced by photosensitizers. Approved porphyrinoid PDT still suffers from lack of robust production methods and low water solubility. Methylene blue (MB) good candidate for the drug, because dye an effective photosensitizer, can be easily synthesized, already being used in other clinical fields. However, its poor cell/tissue penetration stability against reducible biological conditions should addressed proper delivery...
Quantum state tomography is both a crucial component in the field of quantum information and computation formidable task that requires an incogitable number measurement configurations as system dimension grows. We propose experimentally carry out intuitive adaptive compressive scheme, inspired by traditional compressed-sensing protocol signal recovery, tremendously reduces needed to uniquely reconstruct any given without additional priori assumption whatsoever (such rank information, purity,...
A new glioblastoma (GBM) targeting peptide is developed and successfully demonstrated the delivery of a nano-therapeutic.
Posttranslational modifications of hypoxia‐inducible factor‐1α (HIF‐1α) influence HIF‐mediated transcription, likely by affecting binding to p300/cAMP‐response element‐binding protein (CBP). To systematically analyze the HIF‐1α–p300/CBP interaction, we developed a fluorescence polarization‐based assay, employing fluorescein‐labeled peptides derived from C‐terminal transactivation domain (C‐TAD) HIF‐1α. After optimized for effectively capturing p300/CBP, assay was utilized evaluating direct...
We have confirmed that the NO donor (±)-<i>S</i>-nitroso-<i>N</i>-acetylpenicillamine (SNAP) stabilizes transactive form of hypoxia-inducible factor-1α (HIF-1α), leading to induction HIF-1α target genes such as vascular endothelial growth factor and carbonic anhydrase 9. Activation should require inhibition dual system keeps it inactive. One is ubiquitination, which triggered by hydroxylation HIF-1α-proline subsequent binding E3 ubiquitin ligase, von Hippel Lindau (VHL) protein. The other...
Liver-targeted DNA tetrahedron was employed as the carrier for liver-specific delivery of siRNA. Liver siRNA targeting ApoB1 mRNA, which is involved with hypercholesterolemia, lowered blood lipid level.
Aptamer–drug conjugates (ApDCs) are promising anticancer therapeutics with cancer cell specificity.
Abstract The systemic delivery of oligonucleotide therapeutics to the brain is challenging but highly desirable for treatment diseases undruggable with traditional small‐molecule drugs. In this study, a set DNA nanostructures prepared and screened them develop protein corona‐assisted platform therapeutics. biodistribution analysis intravenously injected reveals that cube‐shaped nanostructure (D‐Cb) can penetrate brain‐blood barrier (BBB) reach tissue. distribution level D‐Cb comparable other...